Electron-capture gas chromatography of plasma sulphonylureas after extractive methylation

Hartvig, Per; Fagerlund, Christina; Gyllenhaal, Olle

doi:10.1016/s0378-4347(00)81264-5

Cited by 36 publications

(7 citation statements)

References 15 publications

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“…3 It produces action by blocking potassium channels in the beta cells of islet of langerhans. 4 Existing literature reveals that analytical methods including Gas Chromatography (GC) 5 and High Performance Liquid Chromatographic (HPLC) have been developed for determination Glipizide in human serum 6 as well as in plasma or urine. 7e9 GC method requires derivatization process to form a thermally stable product and thus time consuming.…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of RP-HPLC-UV method for the determination of Glipizide in human plasma

Atif

Khalid

Kit

et al. 2013

Journal of Young Pharmacists

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A simple, sensitive and selective HPLC method with UV detection for determination of Glipizide in human plasma was developed. Liquid-liquid extraction method was used to extract the drug from the plasma samples. Chromatographic separation of Glipizide was achieved using C18 column (ZORBAX ODS 4.6 × 150 mm). The mobile phase was comprised of 0.01 M potassium dihydrogen phosphate and acetonitrile (65:35, v/v) adjusted to pH 4.25 with glacial acetic acid. The analysis was run at a flow rate of 1.5 mL/min with an injection volume was 20 μL. The detector was operated at 275 nm. The calibration curve was linear over a concentration range of 50-1600 ng/mL. Intra-day and inter-day precision and accuracy values were below 15%. The limit of quantification was 50 ng/mL and the mean recovery was above 98%. Freeze-thaw, short-term, long-term and post-preparative stability studies showed that Glipizide in plasma sample was stable. The method may be successfully applied to analyze the Glipizide concentration in plasma samples for bioavailability and bioequivalence studies.

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Section: Introductionmentioning

confidence: 99%

“…7e9 GC method requires derivatization process to form a thermally stable product and thus time consuming. 5 Besides this, GC method lacks specificity as structurally similar sulphonylureas may form identical derivatives. The HPLC methods reported in the literature 6,7 have retention time (RT) of 25 and 10.5 min, respectively.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of RP-HPLC-UV method for the determination of Glipizide in human plasma

Atif

Khalid

Kit

et al. 2013

Journal of Young Pharmacists

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“…The use of dimethyl sulfate is described by Kleber et al (1977), Prescott and Redman (1972), Sabih (1970), Sabih and Sabih (1976), and Simons et al (1972). An extractive methylation involving methyl iodide in methylene chloride is given in the paper by Hartvig et al (1980).…”

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confidence: 99%

Analysis of the herbicide chlorsulfuron in soil by liquid chromatography

Zahnow¹

1982

J. Agric. Food Chem.

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“…Several assay techniques have been described for the determination of glybenclamide in biological fluids, such as spectrophotometry, fluorescence spectroscopy, gas chromatography (GC) [5,6], and high-performance liquid chromatography (HPLC) [1,7]. Atmospheric pressure chemical ionization (APCI) has been used as an interface for liquid chromatography/mass spectrometry (LC/MS) [8,9,10,11].…”

Section: Introductionmentioning

confidence: 99%

LC-APCI-MS-MS methodology for determination of glybenclamide in human plasma

Moura

Nucci

Rath

et al. 2004

Analytical and Bioanalytical Chemistry

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A liquid chromatographic/atmospheric pressure chemical ionization tandem mass spectrometric method (LC-APCI-MS-MS) for the determination of glybenclamide in human plasma is described. Glypizide, an analogue of glybenclamide, was used as internal standard. The analyte was extracted from plasma with diethyl ether/dichloromethane (70:30 v/v). The chromatography uses C18 and 0.01 mol L(-1) acetic acid/acetonitrile (20:80 v/v) as stationary and mobile phase, respectively. Quantitation was preformed by using multiple reaction monitoring (MRM) of the precursor ion (m/z 494.2-->368.8) and the related product ion (m/z 446.0-->347.3) using the internal standard method. The analytical curve was linear in the range 1-300 ng mL(-1), and for a 400-microL sample of human plasma, the limit of determination of the method was 1 ng mL(-1). The coefficients of variation of the method for intra-assay (within-run precision) and inter-assay (between-run precision) were less than 10%. The method was shown to be suitable for pharmacokinetic studies.

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Electron-capture gas chromatography of plasma sulphonylureas after extractive methylation

Cited by 36 publications

References 15 publications

Development and validation of RP-HPLC-UV method for the determination of Glipizide in human plasma

Development and validation of RP-HPLC-UV method for the determination of Glipizide in human plasma

Analysis of the herbicide chlorsulfuron in soil by liquid chromatography

LC-APCI-MS-MS methodology for determination of glybenclamide in human plasma

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