Electrokinetic Mobilities Characterization and Rapid Detection of Microorganisms in Glass Microchannels

Garza-García, Lucía D.; Pérez-González, Víctor H.; Perez-Sanchez, Oscar A.; Lapizco‐Encinas, Blanca H.

doi:10.1002/ceat.201000298

Cited by 12 publications

(6 citation statements)

References 26 publications

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“…Alignment and concentration have been demonstrated for metallic objects like nano wires [6] and gold nanoparticles [7] as well as for DNA molecules and DNA nanostructures (''DNA origami'') [8][9][10]. Both viruses [11,12] and proteins have been locally enriched [13][14][15]. In most cases the optimal experimental conditions appear to have been found empirically.…”

Section: Introductionmentioning

confidence: 94%

Fluid streaming above interdigitated electrodes in dielectrophoresis experiments

2011

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For the investigation of alternating current electrokinetic effects, a system is presented that allows for the simultaneous observation of fluid flow above and around microelectrodes in all three directions in space. Beside the usual microscopical view from top, lateral observation through the same objective is made possible by two small mirrors that are placed next to the electrodes. Fluid flow and movement of fluorescent nanoparticles above interdigitated electrodes are monitored by fluorescence microscopy and digital imaging and are further analysed by image processing. Field frequencies are varied from 10 Hz to 1 GHz at up to 10V(rms) . Electrical conductivity of the fluid is monitored in situ in the actual measuring chamber.

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Section: Introductionmentioning

confidence: 94%

Fluid streaming above interdigitated electrodes in dielectrophoresis experiments

2011

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“…This point can be also extrapolated to microchip‐CE development that it is also expected to keep technologically growing as well as its applications in food analysis. Despite the predominant use of electrochemical detection with microchip CE, novel applications of microchip CE devices and fluorecence detection can be also foreseen in the nondistant future in food analysis . The use of MS is becoming a more frequent alternative detection in CE and it is expected to be also more generally used in food science.…”

Section: Foodomics and Other Future Trends Of Ce In Food Analysismentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2013

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In this work, the analysis of foods and food components using capillary electromigration methods is reviewed. The present work presents and discusses the main CE applications performed in Food Science and Technology including the new field of Foodomics, reviewing recent results on food quality and safety, nutritional value, storage, bioactivity, as well as applications of CE for monitoring food interactions and food processing. The CE analysis of a large variety of food-related molecules with different chemical properties, including amino acids, peptides, proteins, phenolic compounds, carbohydrates, DNA fragments, vitamins, toxins, pesticides, additives, and other minor compounds is described. The use of microchips, CE-MS, and chiral-CE in food analysis is also discussed as well as other current and foreseen trends in this area of research. Following the previous review by Castro-Puyana et al. (Electrophoresis, 2012, 33, 147–167), the current review covers the papers that were published from February 2011 to February 2013.

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“…. These flat channels are commonly used for PIV measurements . Figure shows the total displacement obtained when AC signals starting at ±800 V were applied at the frequencies of 1 Hz (Fig.…”

Section: Resultsmentioning

confidence: 99%

Dynamic microparticle manipulation with an electroosmotic flow gradient in low‐frequency alternating current dielectrophoresis

et al. 2013

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In this study, the potential of low-frequency AC insulator-based DEP (iDEP) was explored for the separation of polystyrene microparticles and yeast cells. An EOF gradient was generated by employing an asymmetrical, 20 Hz AC electrical signal in an iDEP device consisting of a microchannel with diamond-shaped insulating posts. Two types of samples were analyzed, the first sample contained three types of polystyrene particles with different diameters (0.5, 1.0, and 2.0 μm) and the second sample contained two types of polystyrene particles (1.0 and 2 μm) and yeast cells (6.3 μm). This particular scheme uses a tapered AC signal that allows for all particles to be trapped and concentrated at the insulating post array, as the signal becomes asymmetrical (more positive), particles are selectively released. The smallest particles in each sample were released first, since they require greater dielectrophoretic forces to remain trapped. The largest particles in each sample were released last, when the applied signal became cyclical. A dielectropherogram, which is analogous to a chromatogram, was obtained for each sample, demonstrating successful separation of the particles by showing "peaks" of the released particles. These separations were achieved at lower applied potentials than those reported in previous studies that used solely direct current electrical voltages. Additionally, mathematical modeling with COMSOL Multiphysics was carried out to estimate the magnitude of the dielectrophoretic and EOF forces acting on the particles considering the low-frequency, asymmetrical AC signal used in the experiments. The results demonstrated the potential of low-frequency AC-iDEP systems for handling and separating complex mixtures of microparticles and biological cells.

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Electrokinetic Mobilities Characterization and Rapid Detection of Microorganisms in Glass Microchannels

Cited by 12 publications

References 26 publications

Fluid streaming above interdigitated electrodes in dielectrophoresis experiments

Fluid streaming above interdigitated electrodes in dielectrophoresis experiments

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Dynamic microparticle manipulation with an electroosmotic flow gradient in low‐frequency alternating current dielectrophoresis

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