Electrogenerated chemiluminescence of ruthenium (II) bipyridyl complex directly immobilized on glassy carbon electrodes

Abstract

Ru(bpy) 3 Cl 2 was used to modify the glass carbon electrodes (GCE) by oxidation and co-deposition on the electrode surface. The modified electrodes were characterized by atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). About 2.2 9 10 -9 mol Ru(bpy) 3 2? was immobilized on the GCE surface (/ = 4 mm). The modified GC electrodes showed stable electrochemiluminescence with tripropylamine (TPrA) as the co-reactant with a linear range from 10 to 500 lM (R 2 = 0.999). Among the 10 amino acids tested, the modified electrode system showed selective response to arginine and lysine, indicating that the molecular structure played an important role as co-reactant. This simple and sensitive electrode modifying method when combined with flow-injection or liquid chromatography systems has the potential for amino acid analyses.

A solid-state electrochemiluminescence composite modified electrode based on Ru(bpy)32+/PAHNSA: Characterization and pharmaceutical applications

“…These methods can be classified into general approaches including electrostatic adsorption by cationic exchange polymer such as Nafion [6,7], self-assembled monolayer (SAM) [8,9], doping into nanomaterials [10,11], sol-gel [12] and composite films [13,14]. A direct deposition of the luminescent molecule on the carbon electrodes were also adapted [15,16] which believed to enhance the rate of electron transfer kinetics and avoid problems related to the lack of permeability, conductivity, and uniformity of the host membrane.…”

A Sulfhydryl-Reactive Ruthenium (II) Complex and Its Conjugation to Protein G as a Universal Reagent for Fluorescent Immunoassays

“…Ru(II) polypyridine complex is one of the promising chemiluminescent reagents for biosensing due to high chemical stability and reversible reduction/oxidation reactivity [12] , [13] . The electrogenerated chemiluminescence (ECL) using the ruthenium complexes have been widely developed for biosensor construction [5] , [14] , [15] .…”