A Sulfhydryl-Reactive Ruthenium (II) Complex and Its Conjugation to Protein G as a Universal Reagent for Fluorescent Immunoassays

lin, Jing; Chen, Po Chung; Goud, Thirumani Venkatshwar; Huang, Bor Rong; Lin, Tzu Chau; Biellmann, Jean‐François; Chen, Chien Sheng

doi:10.1371/journal.pone.0036086

“…Although it is outside of the scope of this review on peptide-driven luminescent metal complexes, it is important to note that there are also several reports on metal polypyridyl complexes conjugated to proteins. For example, Ru(II) complexes have been conjugated to protein G [ 192 ], cytochrome c [ 193 ] and human serum albumin (HSA) protein [ 194 ] for various applications. Chakrabortty et al presented a protein-Ru(II) hybrid with photosensitising properties which targets the mitochondria [ 194 ].…”

Section: Rationale For Peptide Conjugation To Transition Row Metal Co...mentioning

confidence: 99%

Metal Peptide Conjugates in Cell and Tissue Imaging and Biosensing

Gkika

¹

,

Cullinane

²

,

Keyes

³

2022

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Metal complex luminophores have seen dramatic expansion in application as imaging probes over the past decade. This has been enabled by growing understanding of methods to promote their cell permeation and intracellular targeting. Amongst the successful approaches that have been applied in this regard is peptide-facilitated delivery. Cell-permeating or signal peptides can be readily conjugated to metal complex luminophores and have shown excellent response in carrying such cargo through the cell membrane. In this article, we describe the rationale behind applying metal complexes as probes and sensors in cell imaging and outline the advantages to be gained by applying peptides as the carrier for complex luminophores. We describe some of the progress that has been made in applying peptides in metal complex peptide-driven conjugates as a strategy for cell permeation and targeting of transition metal luminophores. Finally, we provide key examples of their application and outline areas for future progress.

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“…4 Structure of Re(I) complex conjugated to a nuclear localisation signalling (NLS) peptide or a bombesin (BBN) derivative peptide sequence used to improve uptake of the complex by cancer cells overexpressing the gastrin-releasing peptide receptor (GRPR) [152] Although it is outside of the scope of this review on peptide-driven luminescent metal complexes, it is important to note that there are also several reports on metal polypyridyl complexes conjugated to proteins. For example, Ru(II) complexes have been conjugated to protein G [192], cytochrome c [193] and human serum albumin (HSA) protein [194] for various applications. Chakrabortty et al presented a protein-Ru(II) hybrid with photosensitising properties which targets the mitochondria [194].…”

Section: Receptor-targeting Peptidesmentioning

confidence: 99%

Metal peptide conjugates in cell and tissue imaging and biosensing

Gkika

¹

,

Cullinane²,

Keyes³

2022

Topics in Current Chemistry Collections

1

0

View full text Add to dashboard Cite

Metal complex luminophores have seen dramatic expansion in application as imaging probes over the past decade. This has been enabled by growing understanding of methods to promote their cell permeation and intracellular targeting. Amongst the successful approaches that have been applied in this regard is peptide-facilitated delivery. Cell-permeating or signal peptides can be readily conjugated to metal complex luminophores and have shown excellent response in carrying such cargo through the cell membrane. In this article, we describe the rationale behind applying metal complexes as probes and sensors in cell imaging and outline the advantages to be gained by applying peptides as the carrier for complex luminophores. We describe some of the progress that has been made in applying peptides in metal complex peptide-driven conjugates as a strategy for cell permeation and targeting of transition metal luminophores. Finally, we provide key examples of their application and outline areas for future progress.

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“…Covalent tagging of metal complexes has been studied for cell lineage tracing in embryonic cells, to understand the dynamic activities of living cells, probes of phagocytosis and immunoassay and immunofluorescence [ 8 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. The labelling of the proteins has been carried out either by using isothiocyanate complexes for albumins or maleimide complexes for conjugation with cysteine based biomolecules [ 24 ]. Aldehyde-based multiple reactive sites in the ligands of cyclometalated Ir(III) complexes have been used for the crosslinking of primary amines of two proteins [ 10 ].…”

Section: Introductionmentioning

confidence: 99%