Electrochemistry of horse heart cytochrome c

Eddowes, M.J.; Hill, H. Allen O.

doi:10.1021/ja00510a003

Cited by 314 publications

(144 citation statements)

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“…Two stock solutions were prepared as follows: value is in good agreement with literature data [36][37][38][39][40] . From ∆Ep we have determined the value of the standard heterogeneous rate constant for the electron transfer, kº, which amounts to 6.60×10 -4 cm s -1 .…”

Section: Preparation Of Electrodesmentioning

confidence: 62%

Modulation of the Silica Sol–Gel Composition for the Promotion of Direct Electron Transfer to Encapsulated Cytochrome c

et al. 2014

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The direct electron transfer between indium-tin oxide electrodes (ITO) and cytochrome c encapsulated in different sol-gel silica networks was studied. Cyt c@silica modified electrodes were synthesized by a two-step encapsulation method mixing a phosphate buffer solution with dissolved cytochrome c and a silica sol prepared by the alcohol free sol-gel route. These modified electrodes were characterized by cyclic voltammetry, UV-visible spectroscopy and in situ UV-visible spectroelectrochemistry.The electrochemical response of encapsulated protein is influenced by the terminal groups of the silica pores. Cyt c does not present electrochemical response in conventional silica (hydroxyl terminated) or phenyl terminated silica. Direct electron transfer to encapsulated cytochrome c and ITO electrodes only takes place when the protein is encapsulated in methyl modified silica networks.

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Section: Preparation Of Electrodesmentioning

confidence: 62%

Modulation of the Silica Sol–Gel Composition for the Promotion of Direct Electron Transfer to Encapsulated Cytochrome c

et al. 2014

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“…10 According to the literature, the half-wave potential observed at 0.05 V for 0.05 V s -1 is consistent with the native form of the protein. 31 Also, the observation of such potential suggests that there is no change in the protein conformation in consequence of the SAM-Cyt c molecular recognition process. 32,33 Based on the Nicholson's method, 34 the dependence of the difference between the anodic and cathodic peak potentials ( E p ) on the scan rate allows one to estimate the kinetic parameter, .…”

Section: Electroactivity Of the Hpyt Sammentioning

confidence: 98%

5-(4-pyridinyl)-1,3,4-oxadiazole-2-thiol on gold: SAM Formation and electroactivity

Paulo

Silva

Pinheiro

et al. 2008

J. Braz. Chem. Soc.

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O composto 5-(4-pyridinyl)-1,3,4-oxadiazole-2-thiol (Hpyt) adsorve espontaneamente sobre ouro formando SAMs ("Self-Assembled Monolayers") que, de acordo com os resultados eletroquímicos e de STM ("Scanning Tunneling Microscopy"), contêm poros através dos quais as moléculas dos complexos [Fe(CN) 6 ] 4-, a dependência da corrente faradáica com o pH da solução eletrolítica permitiu o cálculo do pKa da molécula de Hpyt adsorvida sobre ouro (4,2). Os parâmetros termodinâmicos, H ads and G ads , para o processo de adsorção desta molécula foram estimados em -20,01 e -39,39 kJ mol -1 , respectivamente, utilizando-se o modelo de Langmuir. O processo redox da metaloproteína citocromo c foi estudado utilizando-se a SAM de Hpyt. A constante de velocidade heterogênea de transferência de elétrons foi calculada em 2,29 10 -3 cm s -1 .5-(4-pyridinyl)-1,3,4-oxadiazole-2-thiol (Hpyt) spontaneously adsorbs on gold forming SAMs (self-assembled monolayers) that, based on STM (Scanning Tunneling Microscopy) and electrochemical data, contain pinholes through which [Fe(CN) 6 ] 4-and [Ru(NH 3 ) 6 ] 3+ probe molecules access the underlying gold electrode. For the former molecule, the dependence of the faradaic current on the electrolyte solution pH value allowed the evaluation of the surface pKa as 4.2. The thermodynamic parameters H ads and G ads for the Hpyt adsorption process could be described by the Langmuir model and were calculated as -20.01 and -39.39 kJ mol -1 , respectively. Electrodic redox reaction of cytochrome c metalloprotein was accessed by using the Hpyt SAM with a heterogeneous electron transfer rate constant of 2.29 10 -3 cm s -1 .

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“…The cytochrome c case is noteworthy because at NEEs, well-resolved CVs are obtained in diluted solutions of the protein both with and without promoters such as 4,4Ј-bipyridyl typically used for promoting cytochrome c electrochemistry (99)(100)(101). These promoters are generally required to avoid adsorption/denaturation (83,102,103) of cytochrome c on the Au surface.…”

Section: Electroanalysis With Neesmentioning

confidence: 99%

Template Deposition of Metals

Ugo

Moretto

2007

Handbook of Electrochemistry

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Template synthesis is a relatively simple and easy procedure which has made the fabrication of rather sophisticated nanomaterials accessible to almost any laboratory. Template synthesis requires access to instrumentation capable of metal sputtering and electrochemical deposition. The characterization of the fabricated nanostructures can be done using instrumental techniques including spectrophotometry, voltammetry, optical microscopy, atomic force microscopy, and electronic microscopies (scanning electron microscopy (SEM) and transmission electron microscopy (TEM)).The method is based on the simple but effective idea that the pores of a host material can be used as a template to direct the growth of new materials. Historically, template synthesis was introduced by Possin (1) and refined by Williams and Giordano (2) who prepared different metallic nanowires with widths as small as 10 nm within the pores of etched nuclear damaged tracks in mica. It was further developed by Martin's group (3-5) and followed by others (6) with the number of examples and applications (7) continually increasing. The nanoporous membranes usually employed as templates are alumina or track-etched polymeric membranes which are widely used as ultrafiltration membranes. Recently, metal nanostructures have also been obtained using the pores created by self-assembly in block copolymer structures under the influence of electric fields and high temperatures (8, 9).The first part of this section focuses on the main characteristics and fabrication techniques used for obtaining templating membranes and depositing metal nanostructures by suitable electroless and electrochemical procedures. Methods such as sol-gel (10-12) or chemical vapor deposition (10, 13), which have been used primarily for the template deposition of carbon, oxides, or semiconducting-based materials, will not be considered here in detail. The second part of the section focuses on the electrochemical properties of the fabricated nanomaterials with emphasis on the characteristics and applications of nanoelectrode ensembles (NEEs). Templating membranes 16.2.2

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Electrochemistry of horse heart cytochrome c

Cited by 314 publications

References 0 publications

Modulation of the Silica Sol–Gel Composition for the Promotion of Direct Electron Transfer to Encapsulated Cytochrome c

Modulation of the Silica Sol–Gel Composition for the Promotion of Direct Electron Transfer to Encapsulated Cytochrome c

5-(4-pyridinyl)-1,3,4-oxadiazole-2-thiol on gold: SAM Formation and electroactivity

Template Deposition of Metals

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