Electrochemistry and chemiluminescence techniques compared in the detection of NADPH oxidase activity in phagocyte cells

Ashkenazi, Avraham; Abu-Rabeah, Khalil; Marks, Robert S.

doi:10.1016/j.talanta.2008.09.035

Cited by 10 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The NADPH-oxidase complex utilizes electrons to produce superoxide radicals from the oxygen molecule [46]. NADPH oxidase may be measured through chemiluminescence [107] or electrochemistry [108] methods, among others. Approaches to quantitate oxygen consumption, extracellular release of O 2 •− or H 2 O 2 , and intracellular O 2 •− production provide reliable assessment of NADPH oxidase activity in a given population of cells [21].…”

Section: Biomarkersmentioning

confidence: 99%

Oxidative Stress-Related Biomarkers in Essential Hypertension and Ischemia-Reperfusion Myocardial Damage

et al. 2013

View full text Add to dashboard Cite

Cardiovascular diseases are a leading cause of mortality and morbidity worldwide, with hypertension being a major risk factor. Numerous studies support the contribution of reactive oxygen and nitrogen species in the pathogenesis of hypertension, as well as other pathologies associated with ischemia/reperfusion. However, the validation of oxidative stress-related biomarkers in these settings is still lacking and novel association of these biomarkers and other biomarkers such as endothelial progenitor cells, endothelial microparticles, and ischemia modified albumin, is just emerging. Oxidative stress has been suggested as a pathogenic factor and therapeutic target in early stages of essential hypertension. Systolic and diastolic blood pressure correlated positively with plasma F2-isoprostane levels and negatively with total antioxidant capacity of plasma in hypertensive and normotensive patients. Cardiac surgery with extracorporeal circulation causes an ischemia/reperfusion event associated with increased lipid peroxidation and protein carbonylation, two biomarkers associated with oxidative damage of cardiac tissue. An enhancement of the antioxidant defense system should contribute to ameliorating functional and structural abnormalities derived from this metabolic impairment. However, data have to be validated with the analysis of the appropriate oxidative stress and/or nitrosative stress biomarkers.

show abstract

Section: Biomarkersmentioning

confidence: 99%

Oxidative Stress-Related Biomarkers in Essential Hypertension and Ischemia-Reperfusion Myocardial Damage

et al. 2013

View full text Add to dashboard Cite

show abstract

“…This method used UV irradiation of quinones to generate reactive oxygen species that were accompanied with the photolytical generation of 3,6-dihydroxyphthalic acid which was detected by luminol. Ashkenazi compared the performances of isoluminol CL and electrochemistry to detect NADPH oxidase activity in human neutrophils and neutrophil-like PLB 985 cells (345). The electrochemical technique was as sensitive as CL and both were able to detect the reactive oxygen species.…”

Section: Chemiluminescencementioning

confidence: 99%

Molecular Fluorescence, Phosphorescence, and Chemiluminescence Spectrometry

et al. 2010

View full text Add to dashboard Cite

“…Unavoidable tissue hypoxia during the operation with subsequent reperfusion increases the production of inflammatory cytokines and ROS [21]. Bugajski et al .…”

Section: Discussionmentioning

confidence: 99%

Comparison of the clinical application of reactive oxygen species and inflammatory markers in patients with endocarditis

Ostrowski¹,

Marcinkiewicz²,

Nowak³

et al. 2012

aoms

View full text Add to dashboard Cite

IntroductionInfective endocarditis (IE) is still connected with high operative mortality. Inflammatory markers are commonly used in monitoring patient clinical condition. Respiratory burst and reactive oxygen species (ROS) are the main way of pathogen elimination. Specificity of this process in the aspect of bacterial infection is the key for correlation assessment between ROS and inflammatory markers in patients with IE. In the study, assessment of ROS as a clinical indicator in IE was conducted.Material and methodsDuring 2007/2008 in the Cardiosurgical Clinic of the Medical University in Lodz there were 20 patients operated on for IE. The examined population consisted of 13 men and 7 women, aged from 23 to 74 years. Inflammatory markers – leukocytosis (WBC), C-reactive protein (CRP), procalcitonin (PCT) and erythrocyte sedimentation rate (ESR) – were assessed preoperatively, on the 3rd, 7th, 12th and 21st day. Simultaneously, with the second venous blood sample chemiluminescence (luminal enhanced whole blood chemiluminescence) was carried out and used to assess ROS production. The results were analyzed statistically.ResultsPositive correlation between ESR, CRP and ROS in the preoperative period was confirmed. An increase in ROS and a statistically significant increase in inflammatory markers on the 3rd day were observed. The ROS normalized on the 12th day. Marked individual variability was specific for the inflammatory markers. Despite the significant decrease, not all of them achieved a normal level at the last control point.ConclusionsAssessment of ROS seems to be a universal parameter with possible application in patients with IE.

show abstract

Electrochemistry and chemiluminescence techniques compared in the detection of NADPH oxidase activity in phagocyte cells

Cited by 10 publications

References 20 publications

Oxidative Stress-Related Biomarkers in Essential Hypertension and Ischemia-Reperfusion Myocardial Damage

Oxidative Stress-Related Biomarkers in Essential Hypertension and Ischemia-Reperfusion Myocardial Damage

Molecular Fluorescence, Phosphorescence, and Chemiluminescence Spectrometry

Comparison of the clinical application of reactive oxygen species and inflammatory markers in patients with endocarditis

Contact Info

Product

Resources

About