Electrochemiluminescent immunosensor for prostate specific antigen based upon luminol functionalized platinum nanoparticles loaded on graphene

Khan, Malik Saddam; Zhu, Wenjuan; Ali, Asghar; Ahmad, Shah Masood; Li, Xiaojian; Yang, Lei; Wang, Yaoguang; Wang, Huan; Wei, Qin

doi:10.1016/j.ab.2018.11.010

Cited by 43 publications

(14 citation statements)

References 48 publications

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“…Electrochemiluminescence (ECL) is a light-emission phenomenon generated at an electrode through highly energetic electron-transfer reactions and implies the resulting combination of electrochemistry and chemiluminescence . Thanks to its specific benefits of a wide dynamic range, fast response, and low background signals, ECL technology has become a growing and significant analytical tool applied broadly in the territory of analytical chemistry, such as food safety analysis, environmental monitoring, , and bioassays. , As most immunoassays reported include time-wasting or a hard labeling procedure, the immunoassay accompanying a label-free construction has attracted widespread attention due to its favorable benefits such as high sensitivity, low cost, and being easy to use. − Till date, several ECL label-free immunosensors have been fabricated to detect miscellaneous biomarkers. , …”

Section: Introductionmentioning

confidence: 99%

Label-Free and Ultrasensitive Electrochemiluminescent Immunosensor Based on Novel Luminophores of Ce₂Sn₂O₇ Nanocubes

2021

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In this research article, a novel and simple label-free electrochemiluminescence (ECL) immunosensor using cerium stannite (Ce2Sn2O7) nanocubes as brand-new ECL emitters has been suggested for the first time. Ce2Sn2O7 nanocubes prepared by a simple hydrothermal method displayed bright ECL emission, promising biocompatibility, low noxiousness, and perfect stability. On comparison of ECL and photoluminescence (PL) spectra, a surface-state mechanism was proposed to be involved in the ECL emission. After aminofunctionalization with 3-aminopropyltriethoxysilane (APTES), Ce2Sn2O7 could be decorated with gold nanoparticles through Au–NH2 covalent linkage, which yielded Au@Ce2Sn2O7 nanocomposites and further enhanced the ECL emission. To confirm the proposed immunosensor feasibility, carcinoembryonic antigen (CEA) was employed as an exemplary analyte. Based on the abovementioned points, our fabricated immunosensor improved the ECL performance to CEA concentrations in a linear range of 0.001–70 ng/mL with a low limit of detection of 0.53 pg/mL (S/N = 3). With outstanding stability, reproducibility, and specificity, this method is expected to be an innovative one for sensitive analyses of CEA and other biomarkers in real samples.

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Section: Introductionmentioning

confidence: 99%

Label-Free and Ultrasensitive Electrochemiluminescent Immunosensor Based on Novel Luminophores of Ce₂Sn₂O₇ Nanocubes

2021

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“…To validate the results, first, a calibration curve was made with a standard solution to then detect the concentration of PSA in human serum. As reported by the authors, the ECL immunosensor is highly accurate, selective, and highly sensitive to PSA, so it would be useful in clinical trials [ 17 ].…”

Section: Platinum Nanoparticles Functionalizationmentioning

confidence: 99%

“…They can also potentiate radiotherapy as they accumulate specifically in the tumor site. Platinum nanoparticles have also been used in the diagnosis of diseases [ 16 , 17 , 18 ]. Therefore, this review focuses on recent reports on the functionalization of platinum nanoparticles with bioactive molecules such as antibodies, biocompatible polymers, peptides, and biomolecules, among others, and their biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Functionalized Platinum Nanoparticles with Biomedical Applications

Rosa

Diaz

Villalobos-Gutiérrez

et al. 2022

IJMS

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Functionalized platinum nanoparticles have been of considerable interest in recent research due to their properties and applications, among which they stand out as therapeutic agents. The functionalization of the surfaces of nanoparticles can overcome the limits of medicine by increasing selectivity and thereby reducing the side effects of conventional drugs. With the constant development of nanotechnology in the biomedical field, functionalized platinum nanoparticles have been used to diagnose and treat diseases such as cancer and infections caused by pathogens. This review reports on physical, chemical, and biological methods of obtaining platinum nanoparticles and the advantages and disadvantages of their synthesis. Additionally, applications in the biomedical field that can be utilized once the surfaces of nanoparticles have been functionalized with different bioactive molecules are discussed, among which antibodies, biodegradable polymers, and biomolecules stand out.

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“…Due to low oxidation potential, strong ECL efficiency, low cost and nontoxicity, luminol has been widely applied in different analytical application. In recent years, the approaches to form solid‐state ECL sensors, by immobilizing the ECL reagent on the surface of electrode, get more attention because of several advantages that solid‐state ECL have over solution‐state ECL, such as less consumption of ECL reagent, better signal‐to‐noise ratio, and simplified experimental design . Luminol as an aniline derivative can be electrochemically polymerized through a linkage of the amino group with the para carbon atom of another molecule .…”

Section: Introductionmentioning

confidence: 99%

An Ultrasensitive ECL Sensor Based on Conducting Polymer/Electrochemically Reduced Graphene Oxide for Non‐Enzymatic Detection in Biological Samples

et al. 2020

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In this paper, we will introduce an enzyme‐free solid‐state electrochemiluminescent (ECL) sensor for ultrasensitive detection of Hydrogen peroxide (normalH2normalO2 ), as a significant molecule in many fundamental biological processes. It was fabricated based on the electro immobilization of poly (Luminol/Aniline) (PLA) nanocomposite on the surface of electrochemically reduced Graphene oxide (ERGO)/CeO2 /GCE modified electrode. We applied electrochemical technique as a controllable, rapid and simple method for the electro copolymerization of Luminol and Aniline and also the electrochemical reduction of graphene oxide (GO). The oxygen functional groups in ERGO were mostly eliminated. The properties of the ERGO/CeO2 /PLA modified electrode were also studied by FE‐SEM, Cyclic voltammetry (CV) and Electrochemical impedance spectroscopy (EIS) methods. Under the optimal conditions, this proposed ECL sensor exhibits high sensitivity and wide linear dynamic range for normalH2normalO2 determination from 0.8 pmolnormalL-1 to 8 mmolnormalL-1 with the low detection limit of 0.36 p molnormalL-1 (S/N=3) and RSD 1.89 %. The results demonstrate that this enzyme‐free solid‐state ECL sensor offers a promising candidate for quantification of normalH2normalO2 concentration in biological fluids such as human urineIntroduction

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Electrochemiluminescent immunosensor for prostate specific antigen based upon luminol functionalized platinum nanoparticles loaded on graphene

Cited by 43 publications

References 48 publications

Label-Free and Ultrasensitive Electrochemiluminescent Immunosensor Based on Novel Luminophores of Ce₂Sn₂O₇ Nanocubes

Label-Free and Ultrasensitive Electrochemiluminescent Immunosensor Based on Novel Luminophores of Ce₂Sn₂O₇ Nanocubes

Functionalized Platinum Nanoparticles with Biomedical Applications

An Ultrasensitive ECL Sensor Based on Conducting Polymer/Electrochemically Reduced Graphene Oxide for Non‐Enzymatic Detection in Biological Samples

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Electrochemiluminescent immunosensor for prostate specific antigen based upon luminol functionalized platinum nanoparticles loaded on graphene

Cited by 43 publications

References 48 publications

Label-Free and Ultrasensitive Electrochemiluminescent Immunosensor Based on Novel Luminophores of Ce2Sn2O7 Nanocubes

Label-Free and Ultrasensitive Electrochemiluminescent Immunosensor Based on Novel Luminophores of Ce2Sn2O7 Nanocubes

Functionalized Platinum Nanoparticles with Biomedical Applications

An Ultrasensitive ECL Sensor Based on Conducting Polymer/Electrochemically Reduced Graphene Oxide for Non‐Enzymatic Detection in Biological Samples

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Label-Free and Ultrasensitive Electrochemiluminescent Immunosensor Based on Novel Luminophores of Ce₂Sn₂O₇ Nanocubes

Label-Free and Ultrasensitive Electrochemiluminescent Immunosensor Based on Novel Luminophores of Ce₂Sn₂O₇ Nanocubes