Label-Free and Ultrasensitive Electrochemiluminescent Immunosensor Based on Novel Luminophores of Ce₂Sn₂O₇ Nanocubes

Abstract

In this research article, a novel and simple label-free electrochemiluminescence (ECL) immunosensor using cerium stannite (Ce2Sn2O7) nanocubes as brand-new ECL emitters has been suggested for the first time. Ce2Sn2O7 nanocubes prepared by a simple hydrothermal method displayed bright ECL emission, promising biocompatibility, low noxiousness, and perfect stability. On comparison of ECL and photoluminescence (PL) spectra, a surface-state mechanism was proposed to be involved in the ECL emission. After aminofunctionalization with 3-aminopropyltriethoxysilane (APTES), Ce2Sn2O7 could be decorated with gold nanoparticles through Au–NH2 covalent linkage, which yielded Au@Ce2Sn2O7 nanocomposites and further enhanced the ECL emission. To confirm the proposed immunosensor feasibility, carcinoembryonic antigen (CEA) was employed as an exemplary analyte. Based on the abovementioned points, our fabricated immunosensor improved the ECL performance to CEA concentrations in a linear range of 0.001–70 ng/mL with a low limit of detection of 0.53 pg/mL (S/N = 3). With outstanding stability, reproducibility, and specificity, this method is expected to be an innovative one for sensitive analyses of CEA and other biomarkers in real samples.

Graphite-like Carbon Nitride Nanotube for Electrochemiluminescence Featuring High Efficiency, High Stability, and Ultrasensitive Ion Detection Capability

“…Similar results were also reported elsewhere and discussed in the same mechanism. , For the possible surface state ECL emission mechanism, although we cannot exclude the possibility at present, it is believed that it is rarely possible in this case . Because, in general, this mechanism typically corresponds to a more significant spectral red shift (i.e., ECL vs FL), which was not observed in this work. , …”

“…42 Because, in general, this mechanism typically corresponds to a more significant spectral red shift (i.e., ECL vs FL), which was not observed in this work. 43,44 A possible K 2 S 2 O 8 -involved ECL mechanism is schematically shown in Figure 4 and the following equations (eqs 1−6). In detail, when a cathodic potential was applied, the S 2 O 8 2− was first reduced to generate strongly oxidizing SO 4…”

Recent Progress in Electrochemical Immunosensors

“…After sequential antibody and antigen treatment, the ECL measurement showed a 0.03 pg/mL LOD, with a 0.0001-100 ng/mL linear range. Khan et al fabricated a labelfree ECL immunosensor using Ce 2 Sn 2 O 7 -AuNP nanocubes for the detection of CEA [106]. For the fabrication of the immunosensor, nanocubes and capture antibodies were sequentially treated.…”

Effects of graphene nanosheets decorated by cerium stannate on the enhancement of flame retardancy and mechanical performances of flexible polyurethane foam composites

“…The broad diffraction peak at 2θ = 25.6° corresponds to the (002) diffraction peak of GNS, indicating that GNS was successfully prepared 37 . A set of 29.14°, 33.77°, 48.51°, 57.59° and 60.41° centered on the 2θ angle, they can be respectively attributed to the (222), (400), (440) (622) and (444) peaks of the pyrochlore structure Ce 2 Sn 2 O 7 with the space group Fd‐3m 38 . Figure 1 reveals the similar profile and diffraction peaks with the CSO pattern, and that the GNS diffraction peak is vanished, indicating the successful immobilization of CSO onto the surface of GNS layers.…”