Electrochemical tuning of alcohol oxidase and dehydrogenase catalysis via biosensing towards butanol-1 detection in fermentation media

Electrochemical operational principles and analytical performance of Pd-based amperometric nanobiosensors

“…Hence, the usage of electroplated Pd-NPs as an electrocatalyst in nanobiosensor design can support the efficient sensing of hydrogen peroxide released during specific enzymatic reaction in the presence of a bioanalyte (see section 3.2.2). at a constant applied potential of −0.08 V. 24 Our results indicate a strong competition in the range of 1 µM-5 mM between the oxygen reduction reaction (ORR) and hydrogen peroxide detection on Pd-NPs, Fig. 1.…”

“…1, see the inset starting from 100 µM) with an increase of loaded analyte concentration (caused by an increase of released hydrogen peroxide as a product of enzymatic reaction), a continuous decrease of the negative current from the hybrid AOx/Nafion/Pd-NPs amperometric biosensors was recorded at least up to the level of 10 mM. 24 To sum it up, the observed strong competition between the ORR and peroxide sensing on Pd-NPs will dramatically affect the electrochemical response and analytical merit of the Pd-NPs-based nanobiosensors with encapsulated oxidases (see section 3.2.2).…”

“…23 In contrast, electrochemical tuning of the enzymatic activity of Pd-NPs-based biosensors towards alcohol detection in fermentation media under very low applied potentials (from −0.02 to −0.32 V) has recently been reported. 24 Hence, to avoid any misinterpretation of the obtained electrochemical results supplied by the one-step produced Pd-NPs-based nanobiosensors, a detailed study on the processes affecting their responses as well as the development of general working principles and operational guidelines are highly necessary.…”

Macroscopically Oriented Magnetic Core‐regularized Nanomaterials for Glucose Biosensors Assisted by Self‐sacrificial Label

“…Moreover, the shell generated by the sacrificing method is PB analogs. PB analogs with high catalysis ability, called "artificial peroxidase" [12], were used to immobilize oxidases [13].However, due to the rapid crystallization rate of PB, there are too many nuclei and rapid crystallization, and regular PB nanostructures cannot be obtained, which limits its application. Therefore, by integrating the unique advantages of the sacrificial method with the regularization of magnetic material nanostructures, it still needs to exploit new formation of magnetic nanomaterial modification for various application.…”

Ceria-Based Nanozymes in Point-of-Care Diagnosis: An Emerging Futuristic Approach for Biosensing

“…Natural enzymes are crucially important building blocks in POC biosensor development, because they can catalyze a series of chemical or biochemical reactions, generating colorimetric or electrochemical signals when exposed to specific analytes. For instance, glucose oxidase, alcohol oxidase, , lactate oxidase, , lactate dehydrogenase, cholesterol oxidase, urate oxidase, horseradish peroxidase (HRP), cytochrome c reductase are those natural enzymes which have been utilized in many biosensing applications. However, some intrinsic shortcomings of natural enzymes still have limitations on their practical applications, including issues related to cost, operational stability, and recycling.…”