Electrochemical study of dopamine and noradrenaline at the water/1,6-dichlorohexane interface

Ribeiro, Danilo Monteiro; Miranda, Inês M.; Silva, A. Fernando; Pereira, Carlos M.

doi:10.1039/c0cp00751j

Cited by 29 publications

(31 citation statements)

References 24 publications

(24 reference statements)

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“…In recent years there have been many reports on the behaviour and detection of biomolecules at the ITIES. The detection of a range of biomolecules including amino acids [19], heparin [20,21], protamine [22,23], haemoglobin [24,25], lysozyme [26,27], insulin [28], dopamine [29][30][31], noradrenaline [31] and DNA [32] have been reported at the ITIES.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical behaviour of myoglobin at an array of microscopic liquid–liquid interfaces

O'Sullivan

Arrigan

2012

Electrochimica Acta

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Electrochemistry at liquid-liquid interfaces, or at interfaces between two immiscible electrolyte solutions (ITIES), provides a basis for the non-redox detection of biological molecules, based on iontransfer or adsorption processes. The electroactivity of myoglobin at an array of micron-sized liquidorganogel interfaces was investigated. The µITIES array was patterned with a silicon membrane consisting of an array of eight pores with radii of ~12.8 µm and a pore to pore separation of ~400 µm.Using cyclic voltammetry at the ITIES, the protein was shown to adsorb at the interface and facilitate the transfer of the organic phase electrolyte anions to the aqueous side of the interface. The electrochemical current response was linear with concentration in the range of 1 -6 µM, with corresponding surface coverage of 10 -50 pmol cm -2 . The reverse peak currents was found to be proportional to the voltammetric scan rate, indicating a desorption process. The detection of the protein was only possibly when the pH of the aqueous phase solution was below the pI of the protein. The steady-state simple ion transfer behaviour of tetraethylammonium cation was decreased on the forward sweep, providing a qualitative indication of the presence of adsorbed protein at the interface.Increasing the ionic strength of the aqueous phase resulted in enhanced peak currents, possibly due to aggregation of protein precipitates in the aqueous solution. UV/Vis absorbance spectroscopy was used 1 ISE Member.2 | P a g e to investigate the effects of various aqueous electrolyte solutions on the structure of the protein, and it was shown that at low pH the protein is at least partially denatured. These results provide the basis for label-free detection of myoglobin at the ITIES.

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Section: Introductionmentioning

confidence: 99%

Electrochemical behaviour of myoglobin at an array of microscopic liquid–liquid interfaces

O'Sullivan

Arrigan

2012

Electrochimica Acta

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“…Its also possible to conclude that the transfer of the NAH + from the aqueous to the organic phase is less favorable when compared to the transfer of DAH + across the interface, since it occurs at more positive potentials. This evidence is related with the more hydrophilic character of NAH + due to the presence of an additional ÀOH group in the molecular structure in relation to DAH + , forming less stable complex with DB18C6 [54]. The simple ion transfer of NAH + and DAH + occurs very near to the positive end of the potential window (data not shown) which is of reduced utility for analytical application.…”

Section: Resultsmentioning

confidence: 70%

“…This ligand is known to form stable complexes with catecholamines by sequestering the protonated amine group within the crown structure [54]. The electrochemical cell used in the quantification studies of the catecholamines is schematically represented in Scheme 1.…”

Section: Resultsmentioning

confidence: 99%

“…[54]): two Ag/AgCl wires were used as reference electrodes (prepared by electrochemical oxidation of an Ag wire in NaCl 1 M solution) and two Pt wires were used as counter electrodes, one in each phase.…”

Section: Methodsmentioning

confidence: 99%

“…This solvent has also the advantage of allowing to obtain a larger potential window when comparing to the use DCE or NB [51]. The interest in this solvent was recently noticeable in the literature [52][53][54].…”

Section: Introductionmentioning

confidence: 97%

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Electrochemical Sensing of Catecholamines at the Water/ 1,6‐Dichlorohexane Interface

2013

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In this work, the analytical use of facilitated ion transfer of catecholamines by dibenzo-18-crown-6 was investigated at the water/1,6-dichlorohexane interface using electrochemical methods (cyclic and square wave voltammetry). The experimental conditions for the analytical determination of noradrenaline and dopamine were established and detection limits of 1.7 and 0.35 mM were obtained, respectively. The effect of excess ascorbic acid on the transfer and detection of the two catecholamines was investigated. The selectivity coefficients obtained for dopamine and noradrenaline in the presence of ascorbic acid were 3.5 10 À4 and 2.5 10 À3 , respectively.

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Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces

2017

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The formation of cytochrome c oligomers was induced at liquid−gel and liquid−liquid interfaces via electroadsorption. At an optimum interfacial potential (Eads=0.975 V), the protein was accumulated at these soft interfaces. It was found that as the concentration of adsorbed protein increased, a single voltammetric peak evolved into double and triple peaks (tads=300 s). Analysis of the protein that accumulated at the interfaces by polyacrylamide gel electrophoresis indicated the presence of oligomeric species, corresponding to dimers (ca. 27 kD), trimers (ca. 35 kD), and even larger species (>250 kD) after prolonged electroadsorption (tads=2 h) at macro‐scale soft interfaces. Accordingly, it was possible to electrochemically induce oligomerisation at these soft interfaces, which can be tuned through experimental factors such as interfacial potential difference, electroadsorption time, and bulk solution concentration. These results suggest the use of electrochemistry at soft interfaces as a strategy for the investigation of protein oligomerisation and its inhibition.

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Electrochemical study of dopamine and noradrenaline at the water/1,6-dichlorohexane interface

Cited by 29 publications

References 24 publications

Electrochemical behaviour of myoglobin at an array of microscopic liquid–liquid interfaces

Electrochemical behaviour of myoglobin at an array of microscopic liquid–liquid interfaces

Electrochemical Sensing of Catecholamines at the Water/ 1,6‐Dichlorohexane Interface

Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces

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