Electrochemical sensor for the sensitive determination of norfloxacin in human urine and pharmaceuticals

Goyal, Rajendra N.; Rana, Anoop Raj Singh; Chasta, Himanshu

doi:10.1016/j.bioelechem.2011.08.006

Cited by 62 publications

(22 citation statements)

References 33 publications

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“…In the last several years, norfloxacin has been ranked as the second-most-prescribed fluoroquinolone antibacterial just next to levofloxacin in China, and it has been widely used in both human and veterinary medicines for the treatment of gonococcal urethritis, respiratory and skin infections (Hernández-Arteseros et al 2002;Chen et al 2006;Goyal et al 2012). The broad use of norfloxacin in animals may result in residues in animal-derived foods and subsequently leads to a public health hazard.…”

Section: Introductionmentioning

confidence: 99%

Determination of Norfloxacin in Food by Capillary Electrophoresis Immunoassay with Laser-Induced Fluorescence Detector

et al. 2014

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A simple and sensitive capillary electrophoresis immunoassay method with laser-induced fluorescence detector was developed for the determination of norfloxacin in food samples. The assay was based on the competitive immunoreaction between fluorescein isothiocyanate-labeled norfloxacin tracer and free norfloxacin with a limited amount of antinorfloxacin antibody. In this study, the experimental parameters affecting the norfloxacin determination were systematically optimized including incubation time, buffer pH and concentration, and separation voltage. Under the optimal conditions, norfloxacin could be accurately quantified within 3 min using Na 2 B 4 O 7 /NaH 2 PO 4 buffer (30 mmol/L, pH 8.0) for background electrolyte and 25 kV for the separation voltage. The linear range of the method was 0.08-50 μg/L with a detection limit of 0.005 μg/L. The relative standard deviations for migration time and peak area of the immunocomplex were 0.17 % (intraday) and 3.46 % (intraday), respectively. The proposed method has been further applied to the determination of norfloxacin in milk, chicken, pork, and fish samples, exhibiting satisfactory recovery and sensitivity. These results indicated that the proposed method might serve as an efficient tool for the selective determination of norfloxacin in food samples.

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Section: Introductionmentioning

confidence: 99%

Determination of Norfloxacin in Food by Capillary Electrophoresis Immunoassay with Laser-Induced Fluorescence Detector

et al. 2014

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“…It is one of the third‐generation members of quinolone antibiotics, fluorinated in position 6 and bearing a piperazinyl moiety in position 7, and is widely applied in medicine. NFLX shows effectual in vitro activity against infections caused by Gram‐positive and Gram‐negative bacteria and Mycoplasma spp., such as pulmonary, urinary and digestive infections . However, fluoroquinolones accumulate in the environment and pose a threat to the ecosystem and human health, since they are widely used in clinical practice .…”

Section: Introductionmentioning

confidence: 99%

“…A number of analytical methods have been developed for the assay of NFLX, such as spectrofluorometry, electrochemical analysis, chemiluminescence, high‐performance liquid chromatography (HPLC), UV–visible spectrophotometry and titrimetry . However, several of these published spectrophotometric and HPLC procedures have disadvantages; for example, they require a heating or extraction step, are time‐consuming, involve expensive reagents and tedious sample pretreatment, show a narrow range of linear response and also need special reagents . Therefore it is necessary to develop a rapid, simple and low‐cost method for the efficient determination of NFLX.…”

Section: Introductionmentioning

confidence: 99%

“…18 However, several of these published spectrophotometric and HPLC procedures have disadvantages; for example, they require a heating or extraction step, are time-consuming, involve expensive reagents and tedious sample pretreatment, show a narrow range of linear response and also need special reagents. 1 Therefore it is necessary to develop a rapid, simple and low-cost method for the efficient determination of NFLX.…”

Section: Introductionmentioning

confidence: 99%

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Determination of norfloxacin in food by an enhanced spectrofluorimetric method

et al. 2016

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“…The spectrophotometric technique is the most widely used in pharmaceutical analysis . Other analytical methods have been used such as spectrofluorimetry , HPLC , electrochemical analysis , luminescence , and CE .…”

Section: Introductionmentioning

confidence: 99%

Multiobjective optimization strategy based on desirability functions used for the microemulsion liquid chromatographic separation and quantification of norfloxacin and tinidazole in plasma and formulations

Abou-Taleb

El‐Wasseef

El-Sherbiny

et al. 2015

J of Separation Science

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The aim of the present study was to optimize a microemulsion liquid chromatography method for the simultaneous determination of norfloxacin and tinidazole binary mixture using a chemometric protocol. Optimization experiments were conducted through a process of screening and optimization. A 2(7-4) fractional factorial design was used as screening design. While the location of optimum conditions was established by applying Derringer's desirability function. The optimal mobile phase composition was predicted to be: 3.5% w/v SDS, 10.03% v/v 1-propanol, 0.5% v/v 1-octanol, and 0.3% triethylamine in 0.02 M phosphoric acid at pH 6.5. The mobile phase was delivered isocratically at a flow rate of 1 mL/min with UV detection at 290 nm. Tinidazole and norfloxacin were eluted with retention times of 1.8 and 5.8 min, respectively. The calibration plots displayed good linear relationships in the concentration ranges of 0.5-50 and 0.75-75 μg/mL for norfloxacin and tinidazole, respectively. The method was successfully applied for determination of both drugs in pharmaceutical dosage forms and real human plasma. Where the accuracy was proved by the low values of % error and high values of recovery, also the relative standard deviation for the results did not exceed 1.5%, proving the precision of the method.

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Electrochemical sensor for the sensitive determination of norfloxacin in human urine and pharmaceuticals

Cited by 62 publications

References 33 publications

Determination of Norfloxacin in Food by Capillary Electrophoresis Immunoassay with Laser-Induced Fluorescence Detector

Determination of Norfloxacin in Food by Capillary Electrophoresis Immunoassay with Laser-Induced Fluorescence Detector

Determination of norfloxacin in food by an enhanced spectrofluorimetric method

Multiobjective optimization strategy based on desirability functions used for the microemulsion liquid chromatographic separation and quantification of norfloxacin and tinidazole in plasma and formulations

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