Electrochemical Hydrogel Lithography of Calcium-Alginate Hydrogels for Cell Culture

Ozawa, Fumisato; Ino, Kosuke; Shiku, Hitoshi; Matsue, Tomokazu

doi:10.3390/ma9090744

Cited by 31 publications

(30 citation statements)

References 21 publications

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“…For this purpose, electrodeposition is a useful technique for addressable and individual electrode arrays. Since electrodeposition is also applied for hydrogel formation (Ozawa et al, 2013a,b, 2016, 2017; Ino et al, 2018a,d; Taira et al, 2018, 2019; Li et al, 2019), biomaterials including cells are easily patterned only on the target electrodes.…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Electric and Electrochemical Microfluidic Devices for Cell Analysis

et al. 2019

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Microfluidic devices are widely used for cell analysis, including applications for single-cell analysis, healthcare, environmental monitoring, and organs-on-a-chip that mimic organs in microfluidics. Moreover, to enable high-throughput cell analysis, real-time monitoring, and non-invasive cell assays, electric and electrochemical systems have been incorporated into microfluidic devices. In this mini-review, we summarize recent advances in these systems, with applications from single cells to three-dimensional cultured cells and organs-on-a-chip. First, we summarize microfluidic devices combined with dielectrophoresis, electrophoresis, and electrowetting-on-a-dielectric for cell manipulation. Next, we review electric and electrochemical assays of cells to determine chemical section activity, and oxygen and glucose consumption activity, among other applications. In addition, we discuss recent devices designed for the electric and electrochemical collection of cell components from cells. Finally, we highlight the future directions of research in this field and their application prospects.

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Section: Conclusion and Prospectsmentioning

confidence: 99%

Electric and Electrochemical Microfluidic Devices for Cell Analysis

et al. 2019

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“…For example, an electrochemical device can be utilized to electrodeposit hydrogels. Cells have been successfully cultured in such hydrogels . By combining biofabrication with electrode arrays into a sensing system, a novel cell culture platform with sensors may be constructed.…”

Section: Perspectives and Conclusionmentioning

confidence: 99%

Intracellular Electrochemical Sensing

et al. 2018

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Observing biochemical processes within living cell is imperative for biological and medical research. Fluoresce imaging is widely used for intracellular sensing of cell membranes, nuclei, lysosomes, and pH. Electrochemical assays have been proposed as an alternative to fluorescence‐based assays because of excellent analytical features of electrochemical devices. Notably, thanks to the rapid progress of micro/nanotechnologies and electrochemical techniques, intracellular electrochemical sensing is making rapid progress, leading to a successful detection of intracellular components. Such insight can provide a deep understanding of cellular biological processes and, ultimately, define the human healthy and diseased states. In this review, we present an overview of recent research progress in intracellular electrochemical sensing. We focus on two main topics, electrochemical extraction of cytosolic contents from cells and intracellular electrochemical sensing in situ.

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“…Briefly, anodic reactions create low pH (high H + ) region near the electrode to solubilize CaCO 3 particles that have been suspended in the alginate solution and the soluble Ca 2+ ions trigger the Ca 2+ ‐responsive alginate to undergo gelation through reversible electrostatic crosslinking. Importantly, CaCO 3 can serve as a buffer that maintains near neutral pH of the Ca 2+ ‐alginate hydrogel, which can be used to co‐deposit prokaryotic and eukaryotic cells . In addition to anodic deposition of Ca 2+ ‐alginate, alginate can also be electrodeposited through a mechanism that involves electrostatic crosslinking by Fe 3+ ions generated electrochemically by Fe 2+ oxidation .…”

Section: Electroaddressable Thin Hydrogel Films To Facilitate Signal mentioning

confidence: 99%

“…The alginate electrodeposition mechanism is particularly useful for integrating cellular functions into the electrode coatings. Alginate hydrogels are biocompatible and routinely used as a scaffold for entrapping cells, while electrodeposition provides the mechanism to assemble cells into organized coatings. For example, sequential Ca 2+ ‐alginate codeposition steps generated an electrode coating with the spatially segregated bacterial populations that allows integration of complex cell‐based functionalities .…”

Section: Electroaddressable Thin Hydrogel Films To Facilitate Signal mentioning

confidence: 99%

Connecting Biology to Electronics: Molecular Communication via Redox Modality

Liu

Tschirhart

et al. 2017

Adv Healthcare Materials

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Biology and electronics are both expert at for accessing, analyzing, and responding to information. Biology uses ions, small molecules, and macromolecules to receive, analyze, store, and transmit information, whereas electronic devices receive input in the form of electromagnetic radiation, process the information using electrons, and then transmit output as electromagnetic waves. Generating the capabilities to connect biology-electronic modalities offers exciting opportunities to shape the future of biosensors, point-of-care medicine, and wearable/implantable devices. Redox reactions offer unique opportunities for bio-device communication that spans the molecular modalities of biology and electrical modality of devices. Here, an approach to search for redox information through an interactive electrochemical probing that is analogous to sonar is adopted. The capabilities of this approach to access global chemical information as well as information of specific redox-active chemical entities are illustrated using recent examples. An example of the use of synthetic biology to recognize external molecular information, process this information through intracellular signal transduction pathways, and generate output responses that can be detected by electrical modalities is also provided. Finally, exciting results in the use of redox reactions to actuate biology are provided to illustrate that synthetic biology offers the potential to guide biological response through electrical cues.

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Electrochemical Hydrogel Lithography of Calcium-Alginate Hydrogels for Cell Culture

Cited by 31 publications

References 21 publications

Electric and Electrochemical Microfluidic Devices for Cell Analysis

Electric and Electrochemical Microfluidic Devices for Cell Analysis

Intracellular Electrochemical Sensing

Connecting Biology to Electronics: Molecular Communication via Redox Modality

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