Electrochemical detection of two tumor markers based on functionalized polypyrrole microspheres as immunoprobes

Zhao, Junqing; Guo, Zilin; Wang, Junchun; Zhang, Yuzhong

doi:10.1039/c6ra01773h

Cited by 13 publications

(1 citation statement)

References 28 publications

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“…Polypyrrole (PPy), as one of attractive conducting polymers, is extensively applied to the design of efficient sensing elements due to its unique properties, such as low cost, easy preparation, tunable conductivity, and biocompatibility [24,25]. Polypyrrole microsphere 5 (PPyMS) obtained through simple oxidative polymerization possessed larger surface area and abundant active sites, indicating redox probes were easy to be immobilized on the surface of PPyMS, and PPyMS had become one of an ideal nanomaterial as immunoprobes [26,27].…”

Section: Many Analytical Methods Have Been Developed For Mc-lr Detectmentioning

confidence: 99%

Ultrasensitive electrochemical microcystin-LR immunosensor using gold nanoparticle functional polypyrrole microsphere catalyzed silver deposition for signal amplification

Zhang

Xiong

Chen

2017

Sensors and Actuators B: Chemical

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This work designed an ultrasensitive electrochemical immunosensor for microcystin-LR (MC-LR) detection using gold nanoparticle functional polypyrrole microsphere (AuNP/PPyMS) as a novel signal tag to induce silver enhancement for signal amplification. The dispersed polypyrrole microsphere (PPyMS) synthesized with a chemical oxidative polymerization was used to load AuNP for labeling signal antibodies, which enhanced the detection signal due to large surface area. The proposed MC-LR immunosensor was constructed by coating a polyethylene glycol film on a carbon nanotubes modified electrode and then immobilizing MC-LR antigen on the film. With a competitive immunoassay format, coated MC-LR competed for MC-LR antibody with added target MC-LR to form antibody-antigen immunocomplex. Subsequently, the immunocomplex reacted with AuNP/PPyMS labeled signal antibodies. The MC-LR immunoreaction monitoring was achieved by the electrochemical stripping signal of the deposited silver catalyzed by AuNP/PPyMS. AuNP/PPyMS-mediated silver enhancement along with carbon nanotubes-promoted electron transfer led to high detection sensitivity of MC-LR. Under optimal conditions, the MC-LR immunoassay had a wide linear range of 0.25 ng L-1 to 50 μg L-1 , low detection limit of 0.1 ng L-1 , and good precision, reproducibility and stability. The designed amplification strategy has potential application in the ultrasensitive electrochemical detection of other algae toxins.

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Section: Many Analytical Methods Have Been Developed For Mc-lr Detectmentioning

confidence: 99%

Ultrasensitive electrochemical microcystin-LR immunosensor using gold nanoparticle functional polypyrrole microsphere catalyzed silver deposition for signal amplification

Zhang

Xiong

Chen

2017

Sensors and Actuators B: Chemical

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A Fe3O4@Au-basedpseudo-homogeneous electrochemical immunosensor for AFP measurement using AFP antibody-GNPs-HRP as detection probe

Yuan

Xue

et al. 2017

Analytical Biochemistry

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Ultrasensitive non enzymatic multiple immunosensor for tumor markers detection by coupling DNA hybridization chain reaction with intercalated molecules

Guo

Wang

Zhang

et al. 2017

Biosensors and Bioelectronics

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Electrochemical detection of two tumor markers based on functionalized polypyrrole microspheres as immunoprobes

Abstract: (A) represents the preparation of the labels and (B) represents the immunosensor preparation and detection principle.

Cited by 13 publications

References 28 publications

Ultrasensitive electrochemical microcystin-LR immunosensor using gold nanoparticle functional polypyrrole microsphere catalyzed silver deposition for signal amplification

Ultrasensitive electrochemical microcystin-LR immunosensor using gold nanoparticle functional polypyrrole microsphere catalyzed silver deposition for signal amplification

A Fe3O4@Au-basedpseudo-homogeneous electrochemical immunosensor for AFP measurement using AFP antibody-GNPs-HRP as detection probe

Ultrasensitive non enzymatic multiple immunosensor for tumor markers detection by coupling DNA hybridization chain reaction with intercalated molecules

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