Ultrasensitive non enzymatic multiple immunosensor for tumor markers detection by coupling DNA hybridization chain reaction with intercalated molecules

Guo, Jinjin; Wang, Junchun; Zhang, Junjun; Zhang, Wenjuan; Zhang, Yu-Zhong

doi:10.1016/j.bios.2016.11.048

Cited by 26 publications

(2 citation statements)

References 28 publications

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“…MetB and Fc were used for the detection of alpha-fetoprotein (AFP) and PSA with LODs of 0.25 pg mL –1 and 0.17 pg mL –1 , respectively . In a similar scenario, HCR was coupled with doxorubicin hydrochloride and MetB in the fabrication of a multiple immunosensor used to detect AFP and CEA with LODs of 0.02 pg mL –1 and 0.03 pg mL –1 , respectively . Both of these studies were performed by the same group; however, in the second approach, a different set of molecules were used as signal reporters, which resulted in higher sensitivity.…”

Section: Protein Detectionmentioning

confidence: 99%

Chemical and Biological Sensing Using Hybridization Chain Reaction

2018

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Since the advent of its theoretical discovery more than 30 years ago, DNA nanotechnology has been used in a plethora of diverse applications in both the fundamental and applied sciences. The recent prominence of DNA-based technologies in the scientific community is largely due to the programmable features stored in its nucleobase composition and sequence, which allow it to assemble into highly advanced structures. DNA nanoassemblies are also highly controllable due to the precision of natural and artificial base-pairing, which can be manipulated by pH, temperature, metal ions, and solvent types. This programmability and molecular-level control have allowed scientists to create and utilize DNA nanostructures in one, two, and three dimensions (1D, 2D, and 3D). Initially, these 2D and 3D DNA lattices and shapes attracted a broad scientific audience because they are fundamentally captivating and structurally elegant; however, transforming these conceptual architectural blueprints into functional materials is essential for further advancements in the DNA nanotechnology field. Herein, the chemical and biological sensing applications of a 1D DNA self-assembly process known as hybridization chain reaction (HCR) are reviewed. HCR is a one-dimensional (1D) double stranded (ds) DNA assembly process initiated only in the presence of a specific short ssDNA (initiator) and two kinetically trapped DNA hairpin structures. HCR is considered an enzyme-free isothermal amplification process, which shows substantial promise and offers a wide range of applications for in situ chemical and biological sensing. Due to its modular nature, HCR can be programmed to activate only in the presence of highly specific biological and/or chemical stimuli. HCR can also be combined with different types of molecular reporters and detection approaches for various analytical readouts. While the long dsDNA HCR product may not be as structurally attractive as the 2D and 3D DNA networks, HCR is highly instrumental for applied biological, chemical, and environmental sciences, and has therefore been studied to foster a variety of objectives. In this review, we have focused on nucleic acid, protein, metabolite, and heavy metal ion detection using this 1D DNA nanotechnology via fluorescence, electrochemical, and nanoparticle-based methodologies.

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Section: Protein Detectionmentioning

confidence: 99%

Chemical and Biological Sensing Using Hybridization Chain Reaction

2018

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“…To effectively improve electrochemical biosensing analysis and make it suitable for quantitative analysis of trace biomolecules, signal amplification strategies like nanoparticle amplification, − biological enzyme catalysis, − molecular biotechnology amplification, − and dendrimer method − are commonly used but still have some drawbacks. In recent years, the polymer-based signal amplification strategy has proved to be effective by labeling the polymer material with a large number of detection probes or active sites for subsequent modification.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive miRNA-21 Biosensor Based on Zn(TCPP) PET-RAFT Polymerization Signal Amplification and Multiple Logic Gate Molecular Recognition

Sun

Liu

Kong

et al. 2023

ACS Appl. Mater. Interfaces

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Quantitative measurement of microRNAs (miRNAs) is extremely important in plenty of biomedical applications especially cancer diagnosis but remains a great challenge. In this work, we developed a logic gate recognition biosensing platform based on the “trinity” molecular recognition mode for quantifying miRNAs with a detection limit of 4.48 aM, along with a linear range from 0.1 nM to 10 aM under optimal experimental conditions. In order to obtain excellent detection performance, we adopted a Zn(TCPP) photocatalytic electron transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization signal amplification strategy. The light-induced PET-RAFT has developed green applications of free radical polymerization in the field of biosensors. This is the first report on the preparation of signal amplification biosensors using PET-RAFT for tumor marker detection. With the outstanding detection performance, we can apply the sensor system to the early screening of lung cancer patients.

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Facilitating In Situ Tumor Imaging with a Tetrahedral DNA Framework‐Enhanced Hybridization Chain Reaction Probe

Zhang

Tian

Xiao

et al. 2022

Adv Funct Materials

114

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Rapid and efficient tools for early cancer detection have diagnostic and therapeutic value. Given that the DNA hairpin-based hybridization chain reaction (HCR) is effective in detecting various biological targets, a tetrahedral framework DNA-enhanced (TDN-enhanced) HCR detection system (T-probe system) is introduced for cancer-related targets and its versatility is demonstrated by detecting intracellular target miRNA 21 and cellular membrane target nucleolin. Benefiting from the spatial confinement of the TDN, the T-probe system demonstrates a high detection rate. It increases the reaction efficiency of nude hairpins in vitro while accurately and rapidly recognizing both membrane and intracellular cancer-related targets in living cells. Furthermore, it exhibits superior fluorescence in vivo within 15 s of peripheral-tumor injection and 10 min of tail-vein injection. The T-probe system efficiently and accurately images tumor-related biomarkers in vitro and in vivo, thereby demonstrating great potential for clinical diagnosis and therapeutic applications.

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Ultrasensitive non enzymatic multiple immunosensor for tumor markers detection by coupling DNA hybridization chain reaction with intercalated molecules

Cited by 26 publications

References 28 publications

Chemical and Biological Sensing Using Hybridization Chain Reaction

Chemical and Biological Sensing Using Hybridization Chain Reaction

Ultrasensitive miRNA-21 Biosensor Based on Zn(TCPP) PET-RAFT Polymerization Signal Amplification and Multiple Logic Gate Molecular Recognition

Facilitating In Situ Tumor Imaging with a Tetrahedral DNA Framework‐Enhanced Hybridization Chain Reaction Probe

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