Ultrasensitive miRNA-21 Biosensor Based on Zn(TCPP) PET-RAFT Polymerization Signal Amplification and Multiple Logic Gate Molecular Recognition

Abstract

Quantitative measurement of microRNAs (miRNAs) is extremely important in plenty of biomedical applications especially cancer diagnosis but remains a great challenge. In this work, we developed a logic gate recognition biosensing platform based on the “trinity” molecular recognition mode for quantifying miRNAs with a detection limit of 4.48 aM, along with a linear range from 0.1 nM to 10 aM under optimal experimental conditions. In order to obtain excellent detection performance, we adopted a Zn(TCPP) photocatalytic electron transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization signal amplification strategy. The light-induced PET-RAFT has developed green applications of free radical polymerization in the field of biosensors. This is the first report on the preparation of signal amplification biosensors using PET-RAFT for tumor marker detection. With the outstanding detection performance, we can apply the sensor system to the early screening of lung cancer patients.

Unleashing the Power of PET-RAFT Polymerization: Journey from Porphyrin-Based Photocatalysts to Combinatorial Technologies and Advanced Bioapplications

“…In addition, the utilization of this strategy exhibits outstanding enzyme-like activity and remarkable biocompatibility, making it a promising tool for precise engineering of biomacromolecules in bioapplications. Utilizing a PET-RAFT polymerization signal amplification strategy, a biosensing platform was devised to quantitatively measure miRNAs, achieving recognition through logic gates, resulting in excellent detection performance . By showcasing its potential for early screening of lung cancer patients and detecting disease markers in clinical settings, the biosensor also plays a role in advancing the field of biological computing systems and novel DNA biosensing technologies.…”

“…Quantitative detection of microRNAs is vital in cancer diagnosis and treatment. PET-RAFT polymerization utilizing ZnTCPP as a photocatalyst enabled the fabrication of signal-amplified biosensors for microRNA detection, which can be employed for early screening of lung cancer patients . Furthermore, research has been conducted on the synthesis of zinc-based porphyrin photocatalysts into a hydrogel network, providing the hydrogel with photooxidation–reduction catalytic abilities under blue- or red-light irradiation .…”

Programmable DNA Nanomachine Integrated with Electrochemically Controlled Atom Transfer Radical Polymerization for Antibody Detection at Picomolar Level

“…Recently, the surface-initiated grafting of polymer chains through atom transfer radical polymerization (ATRP) − has become a potent amplification means. In the electrochemically mediated ATRP (eATRP)-based amplified sensing, ATRP initiators (e.g., alkyl halides) are attached to the sites of biorecognition, such as captured oligonucleotide fragments or carboxylate originating from substrate peptide cleavage by proteases. , The potentiostatic reduction of air-stable Cu (II) deactivators into active Cu­(I) activators then initiates the surface-initiated grafting of polymer chains, resulting in the conscription of numerous signal reporters .…”