Electrochemical Detection of Dopamine Using 3D Porous Graphene Oxide/Gold Nanoparticle Composites

Choo, Sung-Sik; Kang, Ee-Seul; Song, Inmyung; Lee, Dong-Hyun; Choi, Jeong‐Woo; Kim, Tae-Hyung

doi:10.20944/preprints201704.0036.v1

2017

DOI: 10.20944/preprints201704.0036.v1

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Electrochemical Detection of Dopamine Using 3D Porous Graphene Oxide/Gold Nanoparticle Composites

Sung-Sik Choo

Ee-Seul Kang

Inmyung Song

et al.

Abstract: Abstract:The detection of dopamine in a highly sensitive and selective manner is crucial for the early diagnosis of a number of neurological diseases/disorders. Here, a report on a new platform for the electrochemical detection of dopamine with a considerable accuracy that comprises a 3D porous graphene oxide (pGO)/gold nanoparticle (GNP)/pGO composite-modified indium tin oxide (ITO) is presented. The pGO was first synthesized and purified by ultrasonication and centrifugation, and it was then further function… Show more

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Cited by 12 publications

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“…Therefore, diverse approaches for DA detection involving sensor geometry based on PPY have been reported. The most representative methods are electrochemical sensing techniques, which are feasible due to the electroactivity of DA molecules 40,41 . FET-type DA sensors have been suggested; however, these sensors have suffered from a relatively slow response and low selectivity because the sensors were not exclusively specific for DA molecules.…”

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confidence: 99%

High-Performance Conducting Polymer Nanotube-based Liquid-Ion Gated Field-Effect Transistor Aptasensor for Dopamine Exocytosis

Park

Seo

Kim

et al. 2020

Sci Rep

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In this study, ultrasensitive and precise detection of a representative brain hormone, dopamine (DA), was demonstrated using functional conducting polymer nanotubes modified with aptamers. A high-performance aptasensor was composed of interdigitated microelectrodes (IMEs), carboxylated polypyrrole nanotubes (CPNTs) and DA-specific aptamers. The biosensors were constructed by sequential conjugation of CPNTs and aptamer molecules on the IMEs, and the substrate was integrated into a liquid-ion gating system surrounded by pH 7.4 buffer as an electrolyte. To confirm DA exocytosis based on aptasensors, DA sensitivity and selectivity were monitored using liquid-ion gated field-effect transistors (FETs). The minimum detection level (MDL; 100 pM) of the aptasensors was determined, and their MDL was optimized by controlling the diameter of the CPNTs owing to their different capacities for aptamer introduction. The MDL of CPNT aptasensors is sufficient for discriminating between healthy and unhealthy individuals because the total DA concentration in the blood of normal person is generally determined to be ca. 0.5 to 6.2 ng/mL (3.9 to 40.5 nM) by high-performance liquid chromatography (HPLC) (this information was obtained from a guidebook "Evidence-Based Medicine 2018 SCL " which was published by Seoul Clinical Laboratory). The CPNTs with the smaller diameters (CPNT2: ca. 120 nm) showed 100 times higher sensitivity and selectivity than the wider CPNTs (CPNT1: ca. 200 nm). Moreover, the aptasensors based on CPNTs had excellent DA discrimination in the presence of various neurotransmitters. Based on the excellent sensing properties of these aptasensors, the DA levels of exogeneous DA samples that were prepared from PC12 cells by a DA release assay were successfully measured by DA kits, and the aptasensor sensing properties were compared to those of standard DA reagents. Finally, the real-time response values to the various exogeneous DA release levels were similar to those of a standard DA aptasensor. Therefore, CPNT-based aptasensors provide efficient and rapid DA screening for neuron-mediated genetic diseases such as Parkinson's disease.Materials. Pyrrole (Py, 98%) and pyrrole-3-carboxylic acid (Py-COOH, 95%) were purchased from the Aldrich and Acros Organics. A surfactant, sodium bis(2-ethylhexyl)-sulfosuccinate (AOT, 98%) and hexane (98%) were also purchased from the Aldrich. The conjugation reagent, 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)−4methyl-morpholinium chloride (DMT-MM, 96%) was purchased from Aldrich. The aptamer was purchased from Bioneer. Inc. Fabrication of carboxylated polypyrrole nanotubes (CPNTs).CPNTs with different diameters (ca. 120 and 200 nm) were prepared by benchmarks in our previous works 51 . A surfactant, AOT (15 mmol), was dissolved in hexane (40 mL), and the solution was stirred for 30 minutes to reach equilibrium. Subsequently, aqueous FeCl 3 solution (7 M, 1 mL) was introduced into the AOT/hexane solution to generate reverse-cylindrical micelles containing iron cations 55 . Finally, a pyrrole (Py)...

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mentioning

confidence: 99%

High-Performance Conducting Polymer Nanotube-based Liquid-Ion Gated Field-Effect Transistor Aptasensor for Dopamine Exocytosis

Park

Seo

Kim

et al. 2020

Sci Rep

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Cyclophosphazene Intrinsically Derived Heteroatom (S, N, P, O)-Doped Carbon Nanoplates for Ultrasensitive Monitoring of Dopamine from Chicken Samples

et al. 2022

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A novel, metal-free electrode based on heteroatom (S, N, P, O)-doped carbon nanoplates (SNPO-CPL) modifying lead pencil graphite (LPG) has been synthesized by carbonizing a unique heteroatom (S, N, P, O)-containing novel polymer, poly(cyclcotriphosphazene-co-2,5-dioxy-1,4-dithiane) (PCD), for precise screening of dopamine (DA). The designed electrode, SNPO-CPL-800, with optimized percentage of S, N, P, O doping through the sp2-carbon chain, and a large number of surface defects (thus leading to a maximum exposition number of catalytic active sites) led to fast molecular diffusion through the micro-porous structure and facilitated strong binding interaction with the targeted molecules in the interactive signaling transducer at the electrode–electrolyte interface. The designed SNPO-CPL-800 electrode exhibited a sensitive and selective response towards DA monitoring, with a limit of detection (LOD) of 0.01 nM. We also monitored DA levels in commercially available chicken samples using the SNPO-CPL-800 electrode even in the presence of interfering species, thus proving the effectiveness of the designed electrode for the precise monitoring of DA in real samples. This research shows there is a strong potential for opening new windows for ultrasensitive DA monitoring with metal-free electrodes.

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From Enzymatic Dopamine Biosensors to OECT Biosensors of Dopamine

Ravariu

2023

Biosensors

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Neurotransmitters are an important category of substances used inside the nervous system, whose detection with biosensors has been seriously addressed in the last decades. Dopamine, a neurotransmitter from the catecholamine family, was recently discovered to have implications for cardiac arrest or muscle contractions. In addition to having many other neuro-psychiatric implications, dopamine can be detected in blood, urine, and sweat. This review highlights the importance of biosensors as influential tools for dopamine recognition. The first part of this article is related to an introduction to biosensors for neurotransmitters, with a focus on dopamine. The regular methods in their detection are expensive and require high expertise personnel. A major direction of evolution of these biosensors has expanded with the integration of active biological materials suitable for molecular recognition near electronic devices. Secondly, for dopamine in particular, the miniaturized biosensors offer excellent sensitivity and specificity and offer cheaper detection than conventional spectrometry, while their linear detection ranges from the last years fall exactly on the clinical intervals. Thirdly, the applications of novel nanomaterials and biomaterials to these biosensors are discussed. Older generations, metabolism-based or enzymatic biosensors, could not detect concentrations below the micro-molar range. But new generations of biosensors combine aptamer receptors and organic electrochemical transistors, OECTs, as transducers. They have pushed the detection limit to the pico-molar and even femto-molar ranges, which fully correspond to the usual ranges of clinical detection of human dopamine in body humors that cover 0.1 ÷ 10 nM. In addition, if ten years ago the use of natural dopamine receptors on cell membranes seemed impossible for biosensors, the actual technology allows co-integrate transistors and vesicles with natural receptors of dopamine, like G protein-coupled receptors. The technology is still complicated, but the uni-molecular detection selectivity is promising.

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Electrochemical Detection of Dopamine Using 3D Porous Graphene Oxide/Gold Nanoparticle Composites

Cited by 12 publications

References 9 publications

High-Performance Conducting Polymer Nanotube-based Liquid-Ion Gated Field-Effect Transistor Aptasensor for Dopamine Exocytosis

High-Performance Conducting Polymer Nanotube-based Liquid-Ion Gated Field-Effect Transistor Aptasensor for Dopamine Exocytosis

Cyclophosphazene Intrinsically Derived Heteroatom (S, N, P, O)-Doped Carbon Nanoplates for Ultrasensitive Monitoring of Dopamine from Chicken Samples

From Enzymatic Dopamine Biosensors to OECT Biosensors of Dopamine

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