Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

Aamri, Maliana El; Yammouri, Ghita; Mohammadi, Hasna; Amine, Aziz; Korri-Youssoufi, Hafsa

doi:10.3390/bios10110186

Cited by 78 publications

(37 citation statements)

References 193 publications

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“…Due to their specificity and flexibility, hybridization-based probes have also been designed based on the Watson–Crick base-pairing rules to detect complementary targets, such as DNA, mRNA, miRNA, and non-coding RNA [ 49 , 50 ]. For example, linear antisense probes labeled with a fluorophore can be used to detect their complementary DNA or RNA sequences demonstrating their capability in intracellular RNA detection and imaging [ 50 ].…”

Section: Native Nucleic Acidsmentioning

confidence: 99%

Nucleic Acids and Their Analogues for Biomedical Applications

Wang

Chu

et al. 2022

Biosensors

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Nucleic acids are emerging as powerful and functional biomaterials due to their molecular recognition ability, programmability, and ease of synthesis and chemical modification. Various types of nucleic acids have been used as gene regulation tools or therapeutic agents for the treatment of human diseases with genetic disorders. Nucleic acids can also be used to develop sensing platforms for detecting ions, small molecules, proteins, and cells. Their performance can be improved through integration with other organic or inorganic nanomaterials. To further enhance their biological properties, various chemically modified nucleic acid analogues can be generated by modifying their phosphodiester backbone, sugar moiety, nucleobase, or combined sites. Alternatively, using nucleic acids as building blocks for self-assembly of highly ordered nanostructures would enhance their biological stability and cellular uptake efficiency. In this review, we will focus on the development and biomedical applications of structural and functional natural nucleic acids, as well as the chemically modified nucleic acid analogues over the past ten years. The recent progress in the development of functional nanomaterials based on self-assembled DNA-based platforms for gene regulation, biosensing, drug delivery, and therapy will also be presented. We will then summarize with a discussion on the advanced development of nucleic acid research, highlight some of the challenges faced and propose suggestions for further improvement.

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Section: Native Nucleic Acidsmentioning

confidence: 99%

Nucleic Acids and Their Analogues for Biomedical Applications

Wang

Chu

et al. 2022

Biosensors

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“…In electrochemical biosensors, redox mediators are often intercalated between two oligonucleotide strands as signal indicators. The redox mediators could be intercalated via direct and indirect method, where the former relies on the interaction of electroactive species with double-stranded oligonucleotide and the latter involves the creation of recognition sites in oligonucleotide for intercalation [48]. Methylene blue (MB) is a popular electroactive indicator used as DNA intercalating agent as it could interacts with both single-stranded and double-stranded oligonucleotide with different binding modes.…”

Section: Intercalation Of Redox Mediatormentioning

confidence: 99%

Recent Progress in Nanomaterials Modified Electrochemical Biosensors for the Detection of MicroRNA

Low

Chai

et al. 2021

Micromachines

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MicroRNAs (miRNAs) are important non-coding, single-stranded RNAs possessing crucial regulating roles in human body. Therefore, miRNAs have received extensive attention from various disciplines as the aberrant expression of miRNAs are tightly related to different types of diseases. Furthermore, the exceptional stability of miRNAs has presented them as biomarker with high specificity and sensitivity. However, small size, high sequence similarity, low abundance of miRNAs impose difficulty in their detection. Hence, it is of utmost importance to develop accurate and sensitive method for miRNA biosensing. Electrochemical biosensors have been demonstrated as promising solution for miRNA detection as they are highly sensitive, facile, and low-cost with ease of miniaturization. The incorporation of nanomaterials to electrochemical biosensor offers excellent prospects for converting biological recognition events to electronic signal for the development of biosensing platform with desired sensing properties due to their unique properties. This review introduces the signal amplification strategies employed in miRNA electrochemical biosensor and presents the feasibility of different strategies. The recent advances in nanomaterial-based electrochemical biosensor for the detection of miRNA were also discussed and summarized based on different types of miRNAs, opening new approaches in biological analysis and early disease diagnosis. Lastly, the challenges and future prospects are discussed.

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“…Electrochemical biosensors have attracted considerable attention in recent years because of their high sensitivity, simple operation and rapid responsive time [130]. The biointerface and signal reporter are two critical factors for electrochemical biosensors.…”

Section: Electrochemical Cytosensorsmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

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The accurate analysis of circulating tumor cells (CTCs) holds great promise in early diagnosis and prognosis of cancers. However, the extremely low abundance of CTCs in peripheral blood samples limits the practical utility of the traditional methods for CTCs detection. Thus, novel and powerful strategies have been proposed for sensitive detection of CTCs. In particular, nanomaterials with exceptional physical and chemical properties have been used to fabricate cytosensors for amplifying the signal and enhancing the sensitivity. In this review, we summarize the recent development of nanomaterials-based optical and electrochemical analytical techniques for CTCs detection, including fluorescence, colorimetry, surface-enhanced Raman scattering, chemiluminescence, electrochemistry, electrochemiluminescence, photoelectrochemistry and so on.

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Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

Cited by 78 publications

References 193 publications

Nucleic Acids and Their Analogues for Biomedical Applications

Nucleic Acids and Their Analogues for Biomedical Applications

Recent Progress in Nanomaterials Modified Electrochemical Biosensors for the Detection of MicroRNA

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

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