Electrochemical biosensors based on multienzyme systems: Main groups, advantages and limitations – A review

Кучеренко, І. С.; Солдаткін, О. О.; Dzyadevych, S. V.; Солдаткин, А. П.

doi:10.1016/j.aca.2020.03.034

Cited by 79 publications

(49 citation statements)

References 137 publications

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“…Enzymes often significantly increase the rate of reaction and the kinetic parameters can be readily investigated with simple EATs [1,60]. However, these enzymes are often very sensitive to temperature, pH of the solution, and humidity [61]. That is why it is important to maintain optimal conditions while preparing and using this type of sensors.…”

Section: Ecbs Modified With Biocatalystsmentioning

confidence: 99%

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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With the rise in public health awareness, research on point-of-care testing (POCT) has significantly advanced. Electrochemical biosensors (ECBs) are one of the most promising candidates for the future of POCT due to their quick and accurate response, ease of operation, and cost effectiveness. This review focuses on the use of metal nanoparticles (MNPs) for fabricating ECBs that has a potential to be used for POCT. The field has expanded remarkably from its initial enzymatic and immunosensor-based setups. This review provides a concise categorization of the ECBs to allow for a better understanding of the development process. The influence of structural aspects of MNPs in biocompatibility and effective sensor design has been explored. The advances in MNP-based ECBs for the detection of some of the most prominent cancer biomarkers (carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), Herceptin-2 (HER2), etc.) and small biomolecules (glucose, dopamine, hydrogen peroxide, etc.) have been discussed in detail. Additionally, the novel coronavirus (2019-nCoV) ECBs have been briefly discussed. Beyond that, the limitations and challenges that ECBs face in clinical applications are examined and possible pathways for overcoming these limitations are discussed.

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Section: Ecbs Modified With Biocatalystsmentioning

confidence: 99%

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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“…According to the working principle, sensors can be classified as amperometric, potentiometric, conductometric, or impedimetric, among others. [147,148] Guo et al utilized hairpin DNA immobilized on the surface of a gold electrode for sensing EV miR-122. [149] When EV miR-122 was in close proximity to the electrode, it could unzip hairpin DNA and hybridize, forming naked DNA with a short, exposed, unpaired terminal sequence.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Extracellular Vesicles for the Diagnosis of Cancers

Xue

Fan

et al. 2021

Small Structures

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Extracellular vesicles (EVs) are ultrasmall structures secreted by various types of cells and carry biomarkers such as DNA, RNA, and the protein of the cells that secreted them. Cancer cell-derived EVs can be detected at the very early stage of cancers, and hence the use of EVs for cancer diagnosis has aroused significant interest. This review summarizes the representative strategies of using EVs as biomarkers to detect different types of cancers, evaluates their advantages and challenges, and discusses their development prospects for cancer early diagnosis.

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“…Electrochemical biosensors benefit from several advantages, such as low cost, ease of use, portability, and simplicity of construction. These advantages make electrochemical biosensors great options for the development of analytical devices in different fields [3,4]. Some of the limitations for electrochemical biosensors are limited shelf life, narrow or limited temperature range for operation, and sometimes high sensitivity of detection results in false-positive results [5].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensor for Markers of Neurological Esterase Inhibition

2021

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A novel, integrated experimental and modeling framework was applied to an inhibition-based bi-enzyme (IBE) electrochemical biosensor to detect acetylcholinesterase (AChE) inhibitors that may trigger neurological diseases. The biosensor was fabricated by co-immobilizing AChE and tyrosinase (Tyr) on the gold working electrode of a screen-printed electrode (SPE) array. The reaction chemistry included a redox-recycle amplification mechanism to improve the biosensor’s current output and sensitivity. A mechanistic mathematical model of the biosensor was used to simulate key diffusion and reaction steps, including diffusion of AChE’s reactant (phenylacetate) and inhibitor, the reaction kinetics of the two enzymes, and electrochemical reaction kinetics at the SPE’s working electrode. The model was validated by showing that it could reproduce a steady-state biosensor current as a function of the inhibitor (PMSF) concentration and unsteady-state dynamics of the biosensor current following the addition of a reactant (phenylacetate) and inhibitor phenylmethylsulfonylfluoride). The model’s utility for characterizing and optimizing biosensor performance was then demonstrated. It was used to calculate the sensitivity of the biosensor’s current output and the redox-recycle amplification factor as a function of experimental variables. It was used to calculate dimensionless Damkohler numbers and current-control coefficients that indicated the degree to which individual diffusion and reaction steps limited the biosensor’s output current. Finally, the model’s utility in designing IBE biosensors and operating conditions that achieve specific performance criteria was discussed.

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Electrochemical biosensors based on multienzyme systems: Main groups, advantages and limitations – A review

Cited by 79 publications

References 137 publications

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

Extracellular Vesicles for the Diagnosis of Cancers

Electrochemical Biosensor for Markers of Neurological Esterase Inhibition

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