Electrochemical biosensor for p53 gene based on HRP-mimicking DNAzyme-catalyzed deposition of polyaniline coupled with hybridization chain reaction

Ding, Longhua; Zhang, Lina; Yang, Hongmei; Liu, Haiyun; Ge, Shenguang; Yu, Jinghua

doi:10.1016/j.snb.2018.04.126

Cited by 34 publications

(13 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meanwhile, porphyrin-binding aptamers from in vitro selection have attracted some interest as potential catalysts for similar processes, such as peroxidase activity and metal insertion reaction. , Some investigation of the function of these metallo-DNAzymes in living cells has also been reported. For example, the combination of G4/hemin mimicking DNAzyme and hybridization chain reaction has provided a type of electrochemical biosensor to detect the p53 gene; early reports also revealed that the formation of G4/hemin DNAzymes at some genetic regions might play significant roles in the gene regulation or disease diagnosis …”

Section: Results and Discussionsupporting

confidence: 82%

“…37,38 Some investigation of the function of these metallo-DNAzymes in living cells has also been reported. For example, the combination of G4/hemin mimicking DNAzyme and hybridization chain reaction has provided a type of electrochemical biosensor to detect the p53 gene; 39 early reports also revealed that the formation of G4/hemin DNAzymes at some genetic regions might play significant roles in the gene regulation 40 or disease diagnosis. 41 We assessed the peroxidase activity of aptamer-bound hemin (Figure 6A and 6B) by following the oxidative conversion of ABTS to its highly colored radical cation (ABTS •+ ).…”

Section: Acs Combinatorial Sciencementioning

confidence: 99%

See 1 more Smart Citation

Exploration of Catalytic Nucleic Acids on Porphyrin Metalation and Peroxidase Activity by in Vitro Selection of Aptamers for N-Methyl Mesoporphyrin IX

et al. 2019

View full text Add to dashboard Cite

To develop a novel light-up probe and DNAzyme, we selected aptamers for N-methyl mesoporphyrin IX (NMM), a common fluorogenic analogue of coenzyme hemin, by a modified affinity chromatography-based systematic evolution of ligands by exponential enrichment (SELEX). Two truncated aptamers Nm1 and Nm2 with low micromolar dissociation constants (0.75 and 13.27 μM) were obtained after 11 rounds of selection and the final minimized 39-mer aptamer Nm2.1 showed 24-fold fluorescence enhancement for NMM at saturated concentration. Study of the interactions between aptamers and other porphyrin compounds by circular dichroism (CD) and absorption spectroscopy showed that Nm1 mainly assembled as a stem-loop structure, which exhibited a catalytic activity for the metal insertion reaction of mesoporphyrin IX with 3.3-fold rate enhancement. In contrast, the G-rich Nm2 and Nm2.1 were likely to form G-quadruplexes in the presence of alkali metal cations (K + and Na + ), which displayed excellent peroxidase activity exhibiting 19-fold higher catalytic efficiency than hemin alone. The selected aptamers could therefore be used as novel light-up fluorescent probes and DNAzymes by pairing with porphyrin compounds that have potential to construct sensors for various applications.

show abstract

Section: Results and Discussionsupporting

confidence: 82%

Section: Acs Combinatorial Sciencementioning

confidence: 99%

Exploration of Catalytic Nucleic Acids on Porphyrin Metalation and Peroxidase Activity by in Vitro Selection of Aptamers for N-Methyl Mesoporphyrin IX

et al. 2019

View full text Add to dashboard Cite

show abstract

“…By virtue of various nucleases such as nucleic acid endonuclease, exonuclease, and polymerase, the target DNA amount could be indirectly amplified owing to target cycling or generation of target analogs. Enzyme-free nucleic acid assembly strategies such as catalytic hairpin assembly, hybridization chain reaction, and DNA-fueled target recycling are usually based on the cascade toehold-mediated strand displacement reactions for signal amplification toward target DNA recognition events (Lv et al, 2015 ; Ding et al, 2018 ; Karunanayake Mudiyanselage et al, 2018 ). Although these nucleases or enzyme-free DNA strategies could substantially improve the detection limit of the target DNA, the relatively complex or rigorous sequence design or the use of too much DNA fragments or various nucleases for signal amplification increases the assay cost and also the risk for error readouts.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Electrochemical DNA Biosensor Fabrication by Coupling an Integral Multifunctional Zirconia-Reduced Graphene Oxide-Thionine Nanocomposite and Exonuclease I-Assisted Cleavage

Chen

Liu

et al. 2020

Front. Chem.

View full text Add to dashboard Cite

In this work, a simple but sensitive electrochemical DNA biosensor for nucleic acid detection was developed by taking advantage of exonuclease (Exo) I-assisted cleavage for background reduction and zirconia-reduced graphene oxide-thionine (ZrO 2-rGO-Thi) nanocomposite for integral DNA recognition, signal amplification, and reporting. The ZrO 2-rGO nanocomposite was obtained by a one-step hydrothermal synthesis method. Then, thionine was adsorbed onto the rGO surface, via π-π stacking, as an excellent electrochemical probe. The biosensor fabrication is very simple, with probe DNA immobilization and hybridization recognition with the target nucleic acid. Then, the ZrO 2-rGO-Thi nanocomposite was captured onto an electrode via the multicoordinative interaction of ZrO 2 with the phosphate group on the DNA skeleton. The adsorbed abundant thionine molecules onto the ZrO 2-rGO nanocomposite facilitated an amplified electrochemical response related with the target DNA. Since upon the interaction of the ZrO 2-rGO-Thi nanocomposite with the probe DNA an immobilized electrode may also occur, an Exo I-assisted cleavage was combined to remove the unhybridized probe DNA for background reduction. With the current proposed strategy, the target DNA related with P53 gene could be sensitively assayed, with a wide linear detection range from 100 fM to 10 nM and an attractive low detection limit of 24 fM. Also, the developed DNA biosensor could differentiate the mismatched targets from complementary target DNA. Therefore, it offers a simple but effective biosensor fabrication strategy and is anticipated to show potential for applications in bioanalysis and medical diagnosis.

show abstract

“…PANI has been considered as the second most-used conductive polymer in biomedical fields because of the beneficial features such as different structural forms, inexpensiveness, high stability in various environmental conditions, and the capability to electrically shift between resistive and conductive states by applying doping/dedoping procedure. PANI is suitable in electrochromic and supercapacitor, [68] biosensor, [69] NPs, [70] and drug delivery applications. [71] PANI is a homopolymer containing benzenoid or quinonoid or both of them in its structure with various ratio.…”

Section: Properties Structure and Applicationsmentioning

confidence: 99%

Conductive Biomaterials as Substrates for Neural Stem Cells Differentiation towards Neuronal Lineage Cells

Farokhi

Mottaghitalab

Saeb

et al. 2020

Macromolecular Bioscience

View full text Add to dashboard Cite

The injuries and defects in the central nervous system are the causes of disability and death of an affected person. As of now, there are no clinically available methods to enhance neural structural regeneration and functional recovery of nerve injuries. Recently, some experimental studies claimed that the injuries in brain can be repaired by progenitor or neural stem cells located in the neurogenic sites of adult mammalian brain. Various attempts have been made to construct biomimetic physiological microenvironment for neural stem cells to control their ultimate fate. Conductive materials have been considered as one the best choices for nerve regeneration due to the capacity to mimic the microenvironment of stem cells and regulate the alignment, growth, and differentiation of neural stem cells. The review highlights the use of conductive biomaterials, e.g., polypyrrole, polyaniline, poly(3,4‐ethylenedioxythiophene), multi‐walled carbon nanotubes, single‐wall carbon nanotubes, graphene, and graphite oxide, for controlling the neural stem cells activities in terms of proliferation and neuronal differentiation. The effects of conductive biomaterials in axon elongation and synapse formation for optimal repair of central nervous system injuries are also discussed.

show abstract

Electrochemical biosensor for p53 gene based on HRP-mimicking DNAzyme-catalyzed deposition of polyaniline coupled with hybridization chain reaction

Cited by 34 publications

References 49 publications

Exploration of Catalytic Nucleic Acids on Porphyrin Metalation and Peroxidase Activity by in Vitro Selection of Aptamers for N-Methyl Mesoporphyrin IX

Exploration of Catalytic Nucleic Acids on Porphyrin Metalation and Peroxidase Activity by in Vitro Selection of Aptamers for N-Methyl Mesoporphyrin IX

Ultrasensitive Electrochemical DNA Biosensor Fabrication by Coupling an Integral Multifunctional Zirconia-Reduced Graphene Oxide-Thionine Nanocomposite and Exonuclease I-Assisted Cleavage

Conductive Biomaterials as Substrates for Neural Stem Cells Differentiation towards Neuronal Lineage Cells

Contact Info

Product

Resources

About