Exploration of Catalytic Nucleic Acids on Porphyrin Metalation and Peroxidase Activity by in Vitro Selection of Aptamers for <i>N</i>-Methyl Mesoporphyrin IX

Yang, Luyan; Ding, Pi; Luo, Yu; Wang, Jine; Lv, Haiyin; Li, Wenjing; Cao, Yanwei; Pei, Renjun

doi:10.1021/acscombsci.8b00129

Cited by 23 publications

(15 citation statements)

References 42 publications

(67 reference statements)

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“…Thus, the ability of NMM aptamers to accelerate metal insertion (chelatase activity) seems to be positively correlated with their binding affinity for NMM. Nm2 also displayed peroxidase activity in the presence of hemin [13]. Recently, Li et al developed a gold nanoparticle-based SELEX method and identified an aptamer for NMM and ZnPPIX.…”

Section: Discovery Of Nmm As a Gq Ligand And The Development Of Nmm Amentioning

confidence: 99%

N-methyl mesoporphyrin IX as a highly selective light-up probe for G-quadruplex DNA

Yett

Lin

Beseiso

et al. 2019

J. Porphyrins Phthalocyanines

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[Formula: see text]-methyl mesoporphyrin IX (NMM) is a water-soluble, non-symmetric porphyrin with excellent optical properties and unparalleled selectivity for G-quadruplex (GQ) DNA. G-quadruplexes are non-canonical DNA structures formed by guanine-rich sequences. They are implicated in genomic stability, longevity, and cancer. The ability of NMM to selectively recognize GQ structures makes it a valuable scaffold for designing novel GQ binders. In this review, we survey the literature describing the GQ-binding properties of NMM as well as its wide utility in chemistry and biology. We start with the discovery of the GQ-binding properties of NMM and the development of NMM-binding aptamers. We then discuss the optical properties of NMM, focusing on the light-switch effect — high fluorescence of NMM induced upon its binding to GQ DNA. Additionally, we examine the affinity and selectivity of NMM for GQs, as well as its ability to stabilize GQ structures and favor parallel GQ conformations. Furthermore, a portion of the review is dedicated to the applications of NMM-GQ complexes as biosensors for heavy metals, small molecules ([Formula: see text] ATP and pesticides), DNA, and proteins. Finally and importantly, we discuss the utility of NMM as a probe to investigate the roles of GQs in biological processes.

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Section: Discovery Of Nmm As a Gq Ligand And The Development Of Nmm Amentioning

confidence: 99%

N-methyl mesoporphyrin IX as a highly selective light-up probe for G-quadruplex DNA

Yett

Lin

Beseiso

et al. 2019

J. Porphyrins Phthalocyanines

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show abstract

“…After establishment of a DNA oligonucleotide chain library, it is necessary to screen for and enrich oligonucleotide chains that can bind to target molecules with high specificity and affinity. Mature screening techniques include magnetic bead separation[20], high-speed centrifugation, affinity chromatography[21], capillary electrophoresis[22], and nitrocellulose membrane filtration[23]. For example, studies have shown that a target aptamer can be obtained in only four cycles of capillary electrophoresis SELEX[24].…”

Section: Selex Methods For Screening Aptamersmentioning

confidence: 99%

Screening of aptamers and their potential application in targeted diagnosis and therapy of liver cancer

Zhang¹,

Zhong²,

Yang³

et al. 2019

WJG

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Aptamers are a class of single oligonucleotide molecules (DNA or RNA) that are screened from random DNA or RNA oligonucleotide chain libraries by the systemic evolution of ligands by exponential enrichment technology. The selected aptamers are capable of specifically binding to different targeting molecules, which is achieved by the three-dimensional structure of aptamers. Aptamers are similar in function to monoclonal antibodies, and therefore, they are also referred to as "chemical antibodies". Due to their high affinity and specificity and low immunogenicity, aptamers are topics of intense interest in today's biological targeting research especially in tumor research. They not only have high potential for clinical advances in tumor targeting detection but also are highly promising as targeted tumor drug carriers for use in tumor therapy. Various experimental studies have shown that aptamer-based diagnostic and therapeutic methods for liver cancer have great potential for application. This paper summarizes the structure, characteristics, and screening methods of aptamers and reviews the recent research progress on nucleic acid aptamers in the targeted diagnosis and treatment of liver cancer.

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“…Recently, efforts have been made to select non-G4 metalation DNAzymes (Yang et al, 2019), and the effect of metal ions has been examined in more detail. A recent effort obtained a DNA aptamer with a high binding affinity toward MPIX but without a predictable G4 structure.…”

Section: G-quadruplex Containing Dnazymesmentioning

confidence: 99%

Catalytic Nucleic Acids: Biochemistry, Chemical Biology, Biosensors, and Nanotechnology

Liu

2020

iScience

126

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Since the initial discovery of ribozymes in the early 1980s, catalytic nucleic acids have been used in different areas. Compared with protein enzymes, catalytic nucleic acids are programmable in structure, easy to modify, and more stable especially for DNA. We take a historic view to summarize a few main interdisciplinary areas of research on nucleic acid enzymes that may have broader impacts. Early efforts on ribozymes in the 1980s have broken the notion that all enzymes are proteins, supplying new evidence for the RNA world hypothesis. In 1994, the first catalytic DNA (DNAzyme) was reported. Since 2000, the biosensor applications of DNAzymes have emerged and DNAzymes are particularly useful for detecting metal ions, a challenging task for enzymes and antibodies. Combined with nanotechnology, DNAzymes are key building elements for switches allowing dynamic control of materials assembly. The search for new DNAzymes and ribozymes is facilitated by developments in DNA sequencing and computational algorithms, further broadening our fundamental understanding of their biochemistry.

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Exploration of Catalytic Nucleic Acids on Porphyrin Metalation and Peroxidase Activity by in Vitro Selection of Aptamers for N-Methyl Mesoporphyrin IX

Cited by 23 publications

References 42 publications

N-methyl mesoporphyrin IX as a highly selective light-up probe for G-quadruplex DNA

N-methyl mesoporphyrin IX as a highly selective light-up probe for G-quadruplex DNA

Screening of aptamers and their potential application in targeted diagnosis and therapy of liver cancer

Catalytic Nucleic Acids: Biochemistry, Chemical Biology, Biosensors, and Nanotechnology

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