Electrochemical assay of the nitrate and nitrite reductase activities of Rhizobium japonicum

Salome, J. Priscilla; Amutha, R.; Jagannathan, P.; Josiah, J.J.M.; Berchmans, Sheela; Yegnaraman, V.

doi:10.1016/j.bios.2009.04.044

Cited by 11 publications

(8 citation statements)

References 26 publications

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“…The permissible range of nitrite in water is 1 mg/L and the excess of nitrite in water can causes several problems to human such as, blue baby syndrome and shortness of breath202122. Hence, the sensitive and selective detection of nitrite in food, water and environmental samples is more important23. Therefore, several analytical techniques have been developed for the detection of nitrite, such as chemiluminescence, chromatography, spectrometry and capillary electrophoresis242526.…”

mentioning

confidence: 99%

Environmentally friendly synthesis of CeO2 nanoparticles for the catalytic oxidation of benzyl alcohol to benzaldehyde and selective detection of nitrite

Tamizhdurai

Sakthinathan

Chen

et al. 2017

Sci Rep

118

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Cerium oxide nanoparticles (CeO2 NPs) are favorable in nanotechnology based on some remarkable properties. In this study, the crystalline CeO2 NPs are successfully prepared by an efficient microwave combustion (MCM) and conventional route sol-gel (CRSGM) methods. The structural morphology of the as-prepared CeO2 NPs was investigated by various spectroscopic and analytical techniques. Moreover, the XRD pattern confirmed the formation of CeO2 NPs as a face centered cubic structure. The magnetometer studies indicated the low saturation magnetization (23.96 emu/g) of CeO2 NPs for weak paramagnetic and high saturation magnetization (32.13 emu/g) of CeO2 NPs for super paramagnetic. After that, the oxidation effect of benzyl alcohol was investigated which reveals good conversion and selectivity. Besides, the CeO2 NPs modified glassy carbon electrode (GCE) used for the detection of nitrite with linear concentration range (0.02–1200 μM), low limit of detection (0.21 μM) and higher sensitivity (1.7238 μAμM−1 cm−2). However, the CeO2 NPs modified electrode has the fast response, high sensitivity and good selectivity. In addition, the fabricated electrode is applied for the determination of nitrite in various water samples. Eventually, the CeO2 NPs can be regarded as an effective way to enhance the catalytic activity towards the benzyl alcohol and nitrite.

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mentioning

confidence: 99%

Environmentally friendly synthesis of CeO2 nanoparticles for the catalytic oxidation of benzyl alcohol to benzaldehyde and selective detection of nitrite

Tamizhdurai

Sakthinathan

Chen

et al. 2017

Sci Rep

118

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“…Standard calibration curves were prepared with freshly prepared sodium nitrite solution which disproportionates in acid solutions to give NO of the same concentration as described by us earlier. 28 The absence of interference from nitrite has already been verified by us 27 by carrying out the reaction in neutral conditions in the presence of similar concentrations of nitrite where no response was obtained in the presence of ferrocene.…”

Section: Amperometric Measurementsmentioning

confidence: 73%

“…The working potential for amperometry is À0.25 V in the case of the bare GC electrode and À0.22 V in the case of the GC-Au nanocomposite (3-layer) electrode. In our earlier reports 28 the GC electrode was modified by casting a film of Nafion containing ferrocene and this procedure required a higher over-potential of À0.4 V for the amperometric analysis. In the present work, the synergistic influence of the solubilisation of ferrocene and the gold nanocomposite modification can be seen in the lowering of the over-potential required for the NO assay from À0.4 V to À0.22 V. Since the over-potentials are sufficiently negative, the interferences from biologically important molecules like ascorbic acid and uric acid are not present.…”

Section: Amperometric Analysis Of No Using Gc-au Nanocomposite Electrodementioning

confidence: 99%

“…[22][23][24][25][26] Recently we have developed a new protocol for NO sensing based on the ferrocene mediated reduction of NO 27 and we have shown recently how the protocol can be extended for the determination of nitrate and nitrite reductase activities of Rhizobium japonicum. 28 In the protocol developed by us earlier the electrocatalyst ferrocene was modified on a glassy carbon (GC) or Au substrate as a film along with Nafion. In this work we have developed an amperometric method with solubilized ferrocene.…”

Section: Introductionmentioning

confidence: 99%

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Synergistic effect of hydroxypropyl-β-cyclodextrin encapsulated soluble ferrocene and the gold nanocomposite modified glassy carbon electrode for the estimation of NO in biological systems

Varatharajan

Kamaraj

Berchmans

et al. 2010

Analyst

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An electrochemical assay for sensing NO in biological systems is described in this paper. The ferrocene mediated reduction of NO, facilitated by the gold nanocomposite modified glassy carbon electrode is followed by an amperometric procedure. The analytical protocol involves the modification of a glassy carbon electrode by an overlayer of Au nanocomposites prepared through galvanic reduction. Additional overlayers can be built on the surface by repetition of the procedure. The modification leads to the decrease of the over-potential required for the analysis and results in a non-biofouling surface. Since the procedure is based on the electrochemical reduction of NO, the potential interferences from species like dopamine, ascorbic acid, etc., are overcome. The sensitivity, detection limit and response time achieved through this protocol for the modified electrode containing three Au overlayers are 0.03 nA/nM, 25.75 nM and <5 s. Analysis of NO has been carried out in real samples like liver extract, peripheral blood mononuclear cells (PBMCs) and miconazole nitrate ointment and the values obtained are comparable with that obtained by Griess analysis.

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“…Maximum allowable concentrations of 0.5 mg·L −1 for sulfide and 3 mg·L −1 for nitrite are recommended by the World Health Organization [ 9 ]. Various analytical methods have been developed for the detection of individual nitrite and simultaneous nitrite and nitrate anions [ 10 , 11 ]. Also, electrochemical methods with very easy and fast operation have been developed in direct relation with the electrode material and the electrochemical techniques [ 3 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Voltammetric/Amperometric Determination of Sulfide and Nitrite in Water at BDD Electrode

Baciu

Ardelean

Pop

et al. 2015

Sensors

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This work reported new voltammetric/amperometric-based protocols using a commercial boron-doped diamond (BDD) electrode for simple and fast simultaneous detection of sulfide and nitrite from water. Square-wave voltammetry operated under the optimized working conditions of 0.01 V step potential, 0.5 V modulation amplitude and 10 Hz frequency allowed achieving the best electroanalytical parameters for the simultaneous detection of nitrite and sulfide. For practical in-field detection applications, the multiple-pulsed amperometry technique was operated under optimized conditions, i.e., −0.5 V/SCE for a duration of 0.3 s as conditioning step, +0.85 V/SCE for a duration of 3 s that assure the sulfide oxidation and +1.25 V/SCE for a duration of 0.3 s, where the nitrite oxidation occurred, which allowed the simultaneously detection of sulfide and nitrite without interference between them. Good accuracy was found for this protocol in comparison with standardized methods for each anion. Also, no interference effect was found for the cation and anion species, which are common in the water matrix.

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Electrochemical assay of the nitrate and nitrite reductase activities of Rhizobium japonicum

Cited by 11 publications

References 26 publications

Environmentally friendly synthesis of CeO2 nanoparticles for the catalytic oxidation of benzyl alcohol to benzaldehyde and selective detection of nitrite

Environmentally friendly synthesis of CeO2 nanoparticles for the catalytic oxidation of benzyl alcohol to benzaldehyde and selective detection of nitrite

Synergistic effect of hydroxypropyl-β-cyclodextrin encapsulated soluble ferrocene and the gold nanocomposite modified glassy carbon electrode for the estimation of NO in biological systems

Simultaneous Voltammetric/Amperometric Determination of Sulfide and Nitrite in Water at BDD Electrode

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