Gold nanoparticles (AuNPs) have been demonstrated to serve as effective nanomaterial-based enzyme mimetics (nanozymes) for an umber of enzymatic reactions under mild conditions.T he intrinsic glucose oxidase and peroxidase activities of single AuNPs and Ag-Au nanohybrids, respectively,w ere investigated by single NP collision electrochemical measurements.Asignificantly high turnover number of nanozymes was obtained from individual catalytic events compared with the results from the classical, ensembleaveraged measurements.The unusual enhancement of catalytic activity of single nanozymes is believed to originate from the high accessible surface area of monodispersed NPs and the high activities of carbon-supported NPs during single-particle collision at acarbon ultramicroelectrode.This work introduces an ew method for the precise characterization of the intrinsic catalytic activities of nanozymes,g iving further insights to the design of high-efficiency nanomaterial catalysts.