Unveiling the Intrinsic Catalytic Activities of Single‐Gold‐Nanoparticle‐Based Enzyme Mimetics

Hafez, Mahmoud Elsayed; Ma, Hui; Ma, Wei; Long, Yi‐Tao

doi:10.1002/ange.201901384

Cited by 32 publications

(19 citation statements)

References 59 publications

(128 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[11] GNPs possess intrinsic oxidase properties because hydroxide peroxide (H 2 O 2 ) can be generated with the proper physiological components, [12] but the peroxidase properties of GNPs synthesized with phenols (pGNPs) have not been observed from particles with a large size (>20 nm) because of the limited active sites. [13,14] Since ROS decomposed from H 2 O 2 by peroxidase is more lethal to bacteria, it is desirable to produce peroxidase-like properties on pGNPs to promote antibacterial efficiency. It has been observed that the active catechol moiety in phenols provides binding sites for ions such as Fe and the Fenton reaction produces ROS in the presence of H 2 O 2 .…”

mentioning

confidence: 99%

Versatile Phenol‐Incorporated Nanoframes for In Situ Antibacterial Activity Based on Oxidative and Physical Damages

Liu

Mehrjou

et al. 2022

Adv Funct Materials

View full text Add to dashboard Cite

Gold nanoparticles (GNPs) with oxidase and peroxidase properties are great candidates for antibiotic-mimicking materials due to reactive oxygen species (ROS) production. However, the bioenzymic properties are not long-lasting due to the short lifespan of ROS and have only been observed from GNPs with a size of less than 20 nm, thus making the synthesis laborious and inefficient. Herein, GNPs with controllable size and effective ROS utilization are synthesized by an environmentally green process using natural phenols extracted from plants as the reducing and capping reagent. Functional metallic ions are chelated by taking advantage of the coordinating properties of phenols to form the versatile nanoframe (pGNP-Fe) that can self-assemble onto bacteria due to the inherent attraction rendered by phenols, and the physical pressure causes bacterial membrane damage. During internalization in bacteria, the cascade process resulting from the enzyme-like properties generates cytotoxic reactive ROS via oxidization, and the Fenton reaction enhances the antibacterial efficiency. This dual physical/chemical antibacterial process obviates the need for external antibiotics and antibacterial agents, which may otherwise pose toxicity in vivo. The fabrication strategy and materials properties described here provide insights into the design of antibioticmimicking materials based on enzymatic and physical effects.

show abstract

mentioning

confidence: 99%

Versatile Phenol‐Incorporated Nanoframes for In Situ Antibacterial Activity Based on Oxidative and Physical Damages

Liu

Mehrjou

et al. 2022

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…Although the metallic GOx-like NPs yielded >99% gluconic acid, these materials suffered from high adsorption of reaction products resulting in their oxidation and inactivation [212]. The most extensively studied nanooxidases used for selective oxidation of glucose to gluconic acid are AuNPs [4,210,[213][214][215][216][217][218][219][220][221][222] (see Table 8). Gold NPs are much more resistant to O 2 compared with Pt and PdNPs and their reaction products have a lower affinity to adsorption onto the Au surface, in addition to being more active and selective under mild conditions [212].…”

Section: Nanooxidasesmentioning

confidence: 99%

“…They are named "tandem NZs" (nanomaterials with tandem enzyme-like characteristics). Ma and coauthors [222] investigated catalytic properties of single AuNPs and Ag-Au hybrid NPs as possible GOx and PO mimetics. The electrochemical experiments demonstrated that a high turnover of NZs was obtained from individual catalytic elements compared to results from ensemble-averaged measurements as a classic approach.…”

Section: Nanooxidasesmentioning

confidence: 99%

“…Although according to BRENDA data this parameter is also very variable for natural GOs-from 0.005 to 2300 s −1 [231], it is obvious that improving the catalytic efficiency of synthetic NZs is a very important challenge. Nevertheless, the most effective nanooxidases (including those presented in Table 9) can be a good basis for the creation of non-enzymatic sensors for glucose analysis [72,[222][223][224][225][226][227]. Many sensors for glucose detection are based on its electrochemical oxidation directly on a nanocatalyst-covered electrode have been reported (Table 9).…”

Section: Nanooxidasesmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis, Catalytic Properties and Application in Biosensorics of Nanozymes and Electronanocatalysts: A Review

Stasyuk

Smutok

Demkiv

et al. 2020

Sensors

View full text Add to dashboard Cite

The current review is devoted to nanozymes, i.e., nanostructured artificial enzymes which mimic the catalytic properties of natural enzymes. Use of the term “nanozyme” in the literature as indicating an enzyme is not always justified. For example, it is used inappropriately for nanomaterials bound with electrodes that possess catalytic activity only when applying an electric potential. If the enzyme-like activity of such a material is not proven in solution (without applying the potential), such a catalyst should be named an “electronanocatalyst”, not a nanozyme. This paper presents a review of the classification of the nanozymes, their advantages vs. natural enzymes, and potential practical applications. Special attention is paid to nanozyme synthesis methods (hydrothermal and solvothermal, chemical reduction, sol-gel method, co-precipitation, polymerization/polycondensation, electrochemical deposition). The catalytic performance of nanozymes is characterized, a critical point of view on catalytic parameters of nanozymes described in scientific papers is presented and typical mistakes are analyzed. The central part of the review relates to characterization of nanozymes which mimic natural enzymes with analytical importance (“nanoperoxidase”, “nanooxidases”, “nanolaccase”) and their use in the construction of electro-chemical (bio)sensors (“nanosensors”).

show abstract

“…The key issue is the signal-to-noise ratio determined by the background current in circuit or interference from chemical species. Many efforts have been made to resolve the current at lower ranges, such as ultramicroelectrodes 19,20 and surface-enhanced Raman spectroscopy 21,22 ; however, the detection limit is normally restricted in the picoampere to nanoampere range 13,15 . It is highly desirable to develop a new electrochemical detection strategy avoiding above interferences.…”

Section: Introductionmentioning

confidence: 99%

Fermi level-tuned optics of graphene for attocoulomb-scale quantification of electron transfer at single gold nanoparticles

et al. 2019

View full text Add to dashboard Cite

Measurement of electron transfer at single-molecule level is normally restricted by the detection limit of faraday current, currently in a picoampere to nanoampere range. Here we demonstrate a unique graphene-based electrochemical microscopy technique to make an advance in the detection limit. The optical signal of electron transfer arises from the Fermi level-tuned Rayleigh scattering of graphene, which is further enhanced by immobilized gold nanostars. Owing to the specific response to surface charged carriers, graphene-based electrochemical microscopy enables an attoampere-scale detection limit of faraday current at multiple individual gold nanoelectrodes simultaneously. Using the graphene-based electrochemical microscopy, we show the capability to quantitatively measure the attocoulomb-scale electron transfer in cytochrome c adsorbed at a single nanoelectrode. We anticipate the graphene-based electrochemical microscopy to be a potential electrochemical tool for in situ study of biological electron transfer process in organelles, for example the mitochondrial electron transfer, in consideration of the anti-interference ability to chemicals and organisms.

show abstract

Unveiling the Intrinsic Catalytic Activities of Single‐Gold‐Nanoparticle‐Based Enzyme Mimetics

Cited by 32 publications

References 59 publications

Versatile Phenol‐Incorporated Nanoframes for In Situ Antibacterial Activity Based on Oxidative and Physical Damages

Versatile Phenol‐Incorporated Nanoframes for In Situ Antibacterial Activity Based on Oxidative and Physical Damages

Synthesis, Catalytic Properties and Application in Biosensorics of Nanozymes and Electronanocatalysts: A Review

Fermi level-tuned optics of graphene for attocoulomb-scale quantification of electron transfer at single gold nanoparticles

Contact Info

Product

Resources

About