Electrically Regulated Cellular Morphological and Cytoskeletal Changes on an Optically Transparent Electrode

Yaoita, Masashi; Aizawa, Masuo; Ikariyama, Yoshihito

doi:10.1159/000163506

Cited by 16 publications

(12 citation statements)

References 13 publications

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“…Such deleterious effect should be attributed to the conductive nature of metallic implant, which interacts negatively with biological environment . Several in vitro studies had already demonstrated that titanium or chrome‐cobalt submitted to electrical currents presented a cell–electrode interactive effects leading to harmful consequences on cell proliferation, gene expression, protein production, morphology, and cytoskeletal organization resulting often in cell death . Ehrensberger et al indicated that under electrical stimulation within the range of −1000 to −600 mV, the viability of cell cultured on cpTi (commercially pure titanium) markedly decreased .…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of four common orthopedic biomaterials following neuroelectromyostimulation: Anin‐vivostudy

et al. 2017

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Despite the worldwide high prevalence of total joint arthroplasty (TJA), life expectancy of prosthesis remains limited by mechanical and chemical constraint which promote wear debris production, surrounding tissues damage and finally prosthesis loosening. Such results could be amplified by neuro-myoelectrostimulation (NMES; widely used to reduce neuromuscular deficits observed following TJA surgery). It was previously described in an in vivo experiment that interactions between NMES and Ti6Al4V implant are deleterious for both implant and surrounding muscles. The purpose of the present study was to compare the biocompatibility of four common orthopedic biomaterials, two metallic (Ti6Al4V, CrCo) and two nonmetallic (PEEK, Al O ) alloys, fixed on rat tibial crest in which the surrounding muscles were electrostimulated. Muscle cell death rate was not found significantly increased, with or without electrical stimulation for nonmetallic implants. Contrary to Ti6Al4V alloy, the CrCo implant did not induce destruction of the surrounding muscle. However, cell viability decreased for both metallic alloys when NMES was applied but within a greater significant extent for Ti6Al4V implant. Otherwise, when NMES was applied, implant-to-bone adhesion significantly decreased for Ti6Al4V while no significant difference was found for PEEK, Al O , and CrCo. Statistical analyses reveal also a lesser adhesion strength for Ti6Al4V compared with CrCo when NMES was applied. Selecting the most suitable material in term of biocompatibility remains a major concern and non-metallic materials seems to be more appropriated in regard to electrical currents used for post TJA care. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1156-1164, 2018.

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Section: Discussionmentioning

confidence: 99%

Biocompatibility of four common orthopedic biomaterials following neuroelectromyostimulation: Anin‐vivostudy

et al. 2017

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“…When submitted to electrical currents, such compounds are exposed to corrosion reactions, leading to implant surface passive layer abrasion . Numerous studies have demonstrated that titanium or chrome–cobalt submitted to electrical currents in vitro could have cell–electrode interactive effects leading to harmful consequences on cell viability, proliferation, gene expression, protein production, morphology, and cytoskeletal organization, which could result in cell death …”

Section: Introductionmentioning

confidence: 99%

Titanium implant impairment and surrounding muscle cell death following neuro‐myoelectrostimulation: An in vivo study

et al. 2014

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Electrical currents have deleterious effects on biomedical metallic implants. However, following arthroplasty, neuro-myoelectrostimulation (NMES) is often used in patient rehabilitation. Such a rehabilitation technique could compromise patient recovery through deleterious effects on metallic alloys and biological tissues. The purpose of our study was to assess the effects of NMES on a Ti6Al4V implant placed in a rat tibial crest and the surrounding muscle tissues. This in vivo study allowed to bring to the fore the prosthesis behavior under mechanical and electromagnetic loads induced by NEMS stimulation. After 3 weeks, implant-to-bone adhesion significantly decreased in stimulated animals compared with nonstimulated animals. Surface mapping indicated titanium implant degradation after NMES. Furthermore, NMES alone did not induce muscle damage contrary to that found in implanted animals. The muscle damage rate was significantly higher in implanted and stimulated animals compared with implanted-only animals. It seems obvious that rehabilitation programs using the NMES technique could induce early deterioration of biomaterial employed for surgical implants. Clinicians should reconsider the use of NMES as a rehabilitation technique for patients with titanium prostheses.

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“…We think that one of the causes of this result is the difference in activity between broblasts and myoblasts. These days electric or magnetic stimulation is used to induce cellular activity, and this may become the effective method for many tissues, not only muscle tissue (4,9).…”

Section: Discussionmentioning

confidence: 99%

Muscle repair after a transsection injury with development of a gap: an experimental study in rats

Terada

Takayama

Yamada³

et al. 2001

Scandinavian Journal of Plastic and Reconstructive Surgery and

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Lacerated muscle needs fusion with muscle fibres to regain good function, but it often lose its elasticity and the repair seems poor. We think that the scar healing is caused by the development of a gap. In this study in 20 rats we made silicone tube models to keep a constant distance between the muscle ends, and examined the repair inside the silicone tube. In the short gap (1 mm), aligned collagen fibres and muscle fibres bridged both ends. However, when the gap was long (4 mm) they could not bridge both ends and collagen fibres covered the cut ends of muscle. Muscle contains a large fibrous component and will regenerate simultaneously after transsection injury. This result suggests that fibrous repair influences the muscle repair and muscle regeneration will be disturbed as the gap widens.

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Electrically Regulated Cellular Morphological and Cytoskeletal Changes on an Optically Transparent Electrode

Cited by 16 publications

References 13 publications

Biocompatibility of four common orthopedic biomaterials following neuroelectromyostimulation: Anin‐vivostudy

Biocompatibility of four common orthopedic biomaterials following neuroelectromyostimulation: Anin‐vivostudy

Titanium implant impairment and surrounding muscle cell death following neuro‐myoelectrostimulation: An in vivo study

Muscle repair after a transsection injury with development of a gap: an experimental study in rats

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