Muscle repair after a transsection injury with development of a gap: an experimental study in rats

Terada, Nobuki; Takayama, Seiji; Yamada, Harumoto; Seki, Tomohiro

doi:10.1080/028443101750523131

Cited by 33 publications

(10 citation statements)

References 7 publications

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“…Self regeneration is limited in these cases because the remaining myofibers are unable to bridge the gap created by the injury. Any attempt in regeneration results in scar tissue formation in the area of injury or the muscle remodels such that the area of injury becomes permanently devoid of tissue [156, 157]. When evaluating muscle healing capacity in rats, Merritt et al created 0.5 cm × 1 cm or 1 cm × 1 cm full thickness defects in the rat gastrocnemius and found that smaller defects healed by 14 days while the larger defects exhibited no functional recovery after 28 days [157].…”

Section: Skeletal Musclementioning

confidence: 99%

“…When evaluating muscle healing capacity in rats, Merritt et al created 0.5 cm × 1 cm or 1 cm × 1 cm full thickness defects in the rat gastrocnemius and found that smaller defects healed by 14 days while the larger defects exhibited no functional recovery after 28 days [157]. Similarly, Terada et al looked at muscle regeneration in rats in which the ends of lacerated muscle fibers were kept either 1 mm or 4 mm apart using silicone tubes [156]. It was found that muscle fibers were able to bridge the 1 mm gaps with well-aligned collagen fibers.…”

Section: Skeletal Musclementioning

confidence: 99%

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Decellularized tissue and cell-derived extracellular matrices as scaffolds for orthopaedic tissue engineering

Cheng

Solorio

Alsberg

2014

Biotechnology Advances

306

283

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The reconstruction of musculoskeletal defects is a constant challenge for orthopaedic surgeons. Musculoskeletal injuries such as fractures, chondral lesions, infections and tumor debulking can often lead to large tissue voids requiring reconstruction with tissue grafts. Autografts are currently the gold standard in orthopaedic tissue reconstruction; however, there is a limit to the amount of tissue that can be harvested before compromising the donor site. Tissue engineering strategies using allogeneic or xenogeneic decellularized bone, cartilage, skeletal muscle, tendon and ligament have emerged as promising potential alternative treatment. The extracellular matrix provides a natural scaffold for cell attachment, proliferation and differentiation. Decellularization of in vitro cell-derived matrices can also enable the generation of autologous constructs from tissue specific cells or progenitor cells. Although decellularized bone tissue is widely used clinically in orthopaedic applications, the exciting potential of decellularized cartilage, skeletal muscle, tendon and ligament cell-derived matrices has only recently begun to be explored for ultimate translation to the orthopaedic clinic.

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Section: Skeletal Musclementioning

confidence: 99%

Section: Skeletal Musclementioning

confidence: 99%

Decellularized tissue and cell-derived extracellular matrices as scaffolds for orthopaedic tissue engineering

Cheng

Solorio

Alsberg

2014

Biotechnology Advances

306

283

View full text Add to dashboard Cite

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“…Termed volumetric muscle loss (VML), the bulk loss of muscle tissue (>20% by mass) overwhelms the capacity for regeneration, leading to the formation of non-contractile scar tissue at the defect site and functional impairment for the patient (Grogan and others 2011; Terada and others 2001). The poor clinical outcome following VML injury combined with a lack of effective treatment options motivates our exploration of VML regeneration.…”

Section: Introductionmentioning

confidence: 99%

Recovery from volumetric muscle loss injury: A comparison between young and aged rats

Kim

Kasukonis

Brown

et al. 2016

Experimental Gerontology

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Termed volumetric muscle loss (VML), the bulk loss of skeletal muscle tissue either through trauma or surgery overwhelms the capacity for repair, leading to the formation of non-contractile scar tissue. The myogenic potential, along with other factors that influence wound repair are known to decline with age. In order to develop effective treatment strategies for VML injuries that are effective across a broad range of patient populations, it is necessary to understand how the response to VML injury is affected by aging. Towards this end, this study was conducted to compare the response of young and aged animal groups to a lower extremity VML injury. Young (3 months, n=12) and aged (18 months, n=8) male Fischer 344 rats underwent surgical VML injury of the tibialis anterior muscle. Three months after VML injury it was found that young TA muscle was on average 16% heavier than aged muscle when no VML injury was performed and 25% heavier when comparing VML treated young and aged animals (p<0.0001, p<0.0001). Peak contractile force for both the young and aged groups was found to decrease significantly following VML injury, producing 65% and 59% of the contralateral limbs’ peak force, respectively (p<0.0001). However, there were no differences found for peak contractile force based on age, suggesting that VML affects muscle’s ability to repair, regardless of age. In this study, we used the ratio of collagen I to MyoD expression as a metric for fibrosis vs. myogenesis. Decreasing fiber cross-sectional area with advancing age (p<0.005) coupled with the ratio of collagen I to MyoD expression, which increased with age, supports the thought that regeneration is impaired in the aged population in favor of fibrosis (p=0.0241). This impairment is also exacerbated by the contribution of VML injury, where a 77-fold increase in the ratio of collagen I to MyoD was observed in the aged group (p<0.0002). The aged animal model described in this study provides a tool for investigators exploring not only the development of VML injury strategies but also the effect of aging on muscle regeneration.

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“…strains, contusions, and lacerations) cells may be injured, but the underlying extracellular matrix (ECM) is largely intact and repair is robust [1, 2]. However, when significant muscle volume is lost (trauma or surgical resection) the physical and chemical cues provided by the ECM are absent and the defect is instead replaced with non-contractile scar tissue [3]. The current standard of care to replace severely damaged or missing muscle is the transfer of autologous muscle flaps.…”

Section: Introductionmentioning

confidence: 99%

Development of a biological scaffold engineered using the extracellular matrix secreted by skeletal muscle cells

Hurd¹,

Bhatti²,

Walker³

et al. 2015

Biomaterials

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The performance of implantable biomaterials derived from decellularized tissue, including encouraging results with skeletal muscle, suggests that the extracellular matrix (ECM) derived from native tissue has promising regenerative potential. Yet, the supply of biomaterials derived from donated tissue will always be limited, which is why the in-vitro fabrication of ECM biomaterials that mimic the properties of tissue is an attractive alternative. Towards this end, our group has utilized a novel method to collect the ECM that skeletal muscle myoblasts secrete and form it into implantable scaffolds. The cell derived ECM contained several matrix constituents, including collagen and fibronectin that were also identified within skeletal muscle samples. The ECM was organized into a porous network that could be formed with the elongated and aligned architecture observed within muscle samples. The ECM material supported the attachment and in-vitro proliferation of cells, suggesting effectiveness for cell transplantation, and was well tolerated by the host when examined in-vivo. The results suggest that the ECM collection approach can be used to produce biomaterials with compositions and structures that are similar to muscle samples, and while the physical properties may not yet match muscle values, the in-vitro and in-vivo results indicate it may be a suitable first generation alternative to tissue derived biomaterials.

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Muscle repair after a transsection injury with development of a gap: an experimental study in rats

Cited by 33 publications

References 7 publications

Decellularized tissue and cell-derived extracellular matrices as scaffolds for orthopaedic tissue engineering

Decellularized tissue and cell-derived extracellular matrices as scaffolds for orthopaedic tissue engineering

Recovery from volumetric muscle loss injury: A comparison between young and aged rats

Development of a biological scaffold engineered using the extracellular matrix secreted by skeletal muscle cells

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