Electrical pulse-induced electrochemical biosensor for hepatitis E virus detection

Chowdhury, Ankan Dutta; Takemura, Kenshin; Li, Tiancheng; Suzuki, Tetsuro; Park, Enoch Y.

doi:10.1038/s41467-019-11644-5

Cited by 160 publications

(121 citation statements)

References 69 publications

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“…Furthermore, IgG was also detected using EIS method in the same environment and the results resembles the DPV detecting result as shown in Figure S4 of the Supporting Information. 52…”

Section: Resultsmentioning

confidence: 99%

A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection

Barman

Zahed

Sharifuzzaman

et al. 2020

Adv Funct Materials

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Current immunosensors have an insufficient number of binding sites for the recognition of biomolecules, which leads to false positive or negative results. In this research, a facile, cost‐effective, disposable, and highly selective electrochemical immunosensing platform is developed based on cationic polyelectrolyte polyallylamine (PAAMI) anchored laser‐ablated graphene (LAG). Here, for the first time, PAAMI is introduced to stabilize LAG flakes, while retaining the intrinsic thermal and electronic properties of the substrate by noncovalent π–π interaction and electrostatic physical absorption. The sensing platform offers a suitable number of anchoring sites for the immobilized antibodies by providing NH2 functional groups. The proper grafting of PAAMI is confirmed through X‐ray photoelectron spectroscopy and Raman spectroscopy. The immunosensing platform is applied to detect immunoglobulin (IgG) biomarkers as a proof of concept. Under optimized conditions, the sensing platform exhibits a linear range of 0.012–15 and 15–352 ng mL−1 with a limit of detection of 6 pg mL−1 for IgG detection with high selectivity. Based on the analysis, the developed immunosensing platform can be used for point‐of‐care detection of IgG in clinical diagnostic centers. Furthermore, the developed strategy is well suited for the detection of other cancer biomarkers after immobilizing the relevant antibodies.

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“…Furthermore, IgG was also detected using EIS method in the same environment and the results resembles the DPV detecting result as shown in Figure S4 of the Supporting Information. 52…”

Section: Resultsmentioning

confidence: 99%

A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection

Barman

Zahed

Sharifuzzaman

et al. 2020

Adv Funct Materials

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“…Acute hepatitis E (AHE) is caused by hepatitis E virus (HEV) infection, which is endemic in many developing countries because of poor sanitation 1 . The virus is predominantly transmitted by the faecal‐oral route 2 . In some developing countries, HEV infection causes the majority of cases of acute viral hepatitis.…”

Section: Introductionmentioning

confidence: 99%

Altered faecal microbiota on the expression of Th cells responses in the exacerbation of patients with hepatitis E infection

Huang

Ling

et al. 2020

Journal of Viral Hepatitis

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Fulminant hepatitis E may lead to acute liver failure (ALF). Perturbations of intestinal microbiota are related to severe liver disease. To study the correlations between faecal microbiota and the occurrence and exacerbation of hepatitis E virus (HEV) infection, we characterized 24 faecal samples from 12 patients with acute hepatitis E (AHE) and 12 patients with HEV-ALF using high-throughput sequencing. We found both the alpha and beta diversity indices showed no significant differences between the AHE and HEV-ALF groups. Several predominant taxa were significantly different between the AHE and HEV-ALF groups. Most notably, the HEV-ALF group had increased levels of Gammaproteobacteria, Proteobacteria, Xanthomonadceae and Stenotrophomonas, but reduced levels of Firmicutes, Streptococcus, Subdoligranulum and Lactobacillus, compared with the AHE group. The levels of Lactobacillaceae and Gammaproteobacteria could be used to distinguish patients with HEV-ALF from those with AHE. In addition, the level of Th lymphocytes was significantly lower in the HEV-ALF group than in the AHE group. The relative abundances of Lactobacillaceae and Gammaproteobacteria were positively correlated with Th lymphocytes, serum international normalized ratio (INR) and hepatic encephalopathy severity. Moreover, surviving patients had higher levels of Lactobacillus mucosae than deceased patients. Our study demonstrated that the presence of altered faecal microbiota is associated with exacerbation of HEV infection; this finding may be useful for exploring the interactions among faecal microbiota, immune responses, mechanisms of infection and progression in patients with HEV, as well as for the development of novel diagnostic and therapeutic strategies.

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“…This label-free antibody sensor provided a broad linear range of CEA concentration from 0.5 to 1000 ng mL −1 with an LOD of 0.01 ng mL −1 . In 2019, another impedimetric immunosensor exploring the use of these nanomaterials, i.e., N,S-GQDs and Au-PANI nanowires, was constructed by Chowdhury and colleagues for quantifying hepatitis E virus (HEV) [333]. Herein, the WE was implanted with Au-PANI nanowires and N,S-GQDs by a self-assembly and an interfacial polymerization strategy.…”

Section: Impedimetric Gqd Sensorsmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

179

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Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow concentrations of relevant biomarkers, pathogens, toxins and pharmaceuticals in biological matrices. Considering these pathophysiologies, various research works have become necessary to fabricate biosensors for their early diagnosis and treatment, using nanomaterials like quantum dots (QDs). These nanomaterials effectively ameliorate the sensor performance with respect to their reproducibility, selectivity as well as sensitivity. In particular, graphene quantum dots (GQDs), which are ideally graphene fragments of nanometer size, constitute discrete features such as acting as attractive fluorophores and excellent electro-catalysts owing to their photo-stability, water-solubility, biocompatibility, non-toxicity and lucrativeness that make them favorable candidates for a wide range of novel biomedical applications. Herein, we reviewed about 300 biomedical studies reported over the last five years which entail the state of art as well as some pioneering ideas with respect to the prominent role of GQDs, especially in the development of optical, electrochemical and photoelectrochemical biosensors. Additionally, we outline the ideal properties of GQDs, their eclectic methods of synthesis, and the general principle behind several biosensing techniques.

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Electrical pulse-induced electrochemical biosensor for hepatitis E virus detection

Cited by 160 publications

References 69 publications

A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection

A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection

Altered faecal microbiota on the expression of Th cells responses in the exacerbation of patients with hepatitis E infection

Applications of Graphene Quantum Dots in Biomedical Sensors

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