Electrical Control of Protein Conformation

Wan, Alwin M. D.; Schur, Rebecca; Ober, Christopher K.; Fischbach, Claudia; Gourdon, Delphine; Malliaras, George G.

doi:10.1002/adma.201200436

Cited by 67 publications

(102 citation statements)

References 26 publications

(26 reference statements)

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“…A gradient in the surface potential was established by applying ±1.0 V across the electrode while the electrode was incubated for 1 h in cell culture medium containing Fn (Figure 6c and 6d). [190] …”

Section: Electroactive Nanomaterials For Electrode-tissue Interfacesmentioning

confidence: 99%

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A Review of Organic and Inorganic Biomaterials for Neural Interfaces

et al. 2014

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Recent advances in nanotechnology have generated wide interest in applying nanomaterials for neural prostheses. An ideal neural interface should create seamless integration into the nervous system and performs reliably for long periods of time. As a result, many nanoscale materials not originally developed for neural interfaces become attractive candidates to detect neural signals and stimulate neurons. In this comprehensive review, an overview of state-of-the-art microelectrode technologies provided first, with focus on the material properties of these microdevices. The advancements in electro active nanomaterials are then reviewed, including conducting polymers, carbon nanotubes, graphene, silicon nanowires, and hybrid organic-inorganic nanomaterials, for neural recording, stimulation, and growth. Finally, technical and scientific challenges are discussed regarding biocompatibility, mechanical mismatch, and electrical properties faced by these nanomaterials for the development of long-lasting functional neural interfaces.

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Section: Electroactive Nanomaterials For Electrode-tissue Interfacesmentioning

confidence: 99%

“…c,d) Reproduced with permission. [190] Copyright 2012, Wiley-VCH. e) SEM images at 15,000×magnification of NGF entrapped in PEDOT films compared with control films produced without NGF modification of the electrolyte.…”

Section: Figurementioning

confidence: 99%

A Review of Organic and Inorganic Biomaterials for Neural Interfaces

et al. 2014

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“…6 Moreover, the cyto-compatibility of PEDOT:PSS has enabled its use in various in vivo and in vitro platforms. 7,8 In particular, previous reports showed that 2D thin films of PEDOT:tosylate, a similar conducting polymer, could electrochemically control protein conformation, 9 and cell secretions. 10 In recent years, several different strategies have been employed to develop porous PEDOT-based materials for applications in sensing, power supply, capacitance, and electrical storage.…”

Section: Introductionmentioning

confidence: 99%

3D conducting polymer platforms for electrical control of protein conformation and cellular functions

et al. 2015

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We report the fabrication of three dimensional (3D) macroporous scaffolds made from poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) via an ice-templating method. The scaffolds offer tunable pore size and morphology, and are electrochemically active. When a potential is applied to the scaffolds, reversible changes take place in their electrical doping state, which in turn enables precise control over the conformation of adsorbed proteins (e.g., fibronectin). Additionally, the scaffolds support the growth of mouse fibroblasts (3T3-L1) for 7 days, and are able to electrically control cell adhesion and pro-angiogenic capability. These 3D matrix-mimicking platforms offer precise control of protein conformation and major cell functions, over large volumes and long cell culture times. As such, they represent a new tool for biological research with many potential applications in bioelectronics, tissue engineering, and regenerative medicine.

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“…For example, 3T3-L1 fibroblast-adipose cells deposited on a PEDOT:tosylate polymer that had a potential bias of -1V to +1V distributed with a preference for cell adhesion toward the positive bias end. It was found that the amount of protein adsorption decreased 18,19 but had a greater propensity to adopt a more unfolded conformation 20 along the gradient toward the positive bias end of increased cell adhesion. A similar effect was observed with neural stem cells on a PEDOT:tosylate electrode with a 2-fold increase in cell adhesion on the oxidized polymer even though the protein adsorption was lower compared to the reduced polymer 21 .…”

Section: Introductionmentioning

confidence: 99%

“…surface potential and conductivity) 29,30 , that have been shown to display nanoscale lateral variation are expected to amplify variations in the conformation and adhesion of individual proteins across the polymer surface. Whilst the above studies have focused on conformation 17,20,21 , the FN-polymer interfacial forces are also interest as they play an important role in force-dependent signal transduction processes such as cellular forces exerted on FN through cell receptors and intracellular proteins to regulate cell function.…”

Section: Introductionmentioning

confidence: 99%

Quantifying fibronectin adhesion with nanoscale spatial resolution on glycosaminoglycan doped polypyrrole using Atomic Force Microscopy

Gelmi

Higgins

Wallace

2013

Biochimica et Biophysica Acta (BBA) - General Subjects

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The interaction of ECM proteins is critical in determining the performance of materials used in biomedical applications such as tissue regeneration, implantable bionics and biosensing. Methods: To improve our understanding of ECM protein-conducting polymer interactions, we have used Atomic Force Microscopy (AFM) to elucidate the interactions of fibronectin (FN) on polypyrrole (PPy) doped with different glycosaminoglycans. Results: We were able to classify four main types of FN interactions, including those related to 1) non-specific adhesion, 2) protein unfolding and subsequent unbinding from the surface, 3) desorption and 4) interactions with no adhesion. FN adhesion on PPy/hyaluronic acid showed a significantly lower density of surface adhesion with the adhesion restricted to nodule structures, as opposed to their peripheries, of the polymer morphology. In contrast, PPy/chondroitin sulfate showed a significantly higher density of surface adhesion to the point where the distribution of adhesion effectively masked the topography. Through conductive AFM imaging, we found that the conductive regions correlated with regions of FN adhesion. Conclusions: Given that the conductivity requires doping of the polymer, these findings suggest that FN adhesion is mediated by interactions with chondroitin sulfate and hyaluronic acid at the polymer surface and may be indicative of specific interactions due to contributions from electrostatic attraction between the FN and sulfate/anionic groups of the dopants. General significance: This study demonstrates the ability of AFM to resolve the protein-conducting polymer interactions at the molecular and nanoscale level, which will be important for interfacing these polymer materials with biological systems. This article is part of a Special Issue entitled Organic Bioelectronics -Novel Applications in Biomedicine.

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Electrical Control of Protein Conformation

Cited by 67 publications

References 26 publications

A Review of Organic and Inorganic Biomaterials for Neural Interfaces

A Review of Organic and Inorganic Biomaterials for Neural Interfaces

3D conducting polymer platforms for electrical control of protein conformation and cellular functions

Quantifying fibronectin adhesion with nanoscale spatial resolution on glycosaminoglycan doped polypyrrole using Atomic Force Microscopy

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