3D conducting polymer platforms for electrical control of protein conformation and cellular functions

Wan, Alwin M. D.; Inal, Sahika; Williams, Tiffany V.; Wang, Karin; Leleux, Pierre; Estevez, Luis; Giannelis, Emmanuel P.; Fischbach, Claudia; Malliaras, George G.; Gourdon, Delphine

doi:10.1039/c5tb00390c

Cited by 116 publications

(124 citation statements)

References 59 publications

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“…Integration of the devices on flexible substrates has already been demonstrated 29 and 3D electrode formats are in progress 63,64 . Here we demonstrated the use of the OECT impedance sensor for the extraction of the cell layer resistance and capacitance in real time while the epithelium was stimulated by the biomechanical action of the fluid flow.…”

Section: Discussionmentioning

confidence: 99%

Organic transistor platform with integrated microfluidics for in-line multi-parametric in vitro cell monitoring

et al. 2017

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Future drug discovery and toxicology testing could benefit significantly from more predictive and multi-parametric readouts from in vitro models. Despite the recent advances in the field of microfluidics, and more recently organ-on-a-chip technology, there is still a high demand for real-time monitoring systems that can be readily embedded with microfluidics. In addition, multi-parametric monitoring is essential to improve the predictive quality of the data used to inform clinical studies that follow. Here we present a microfluidic platform integrated with in-line electronic sensors based on the organic electrochemical transistor. Our goals are twofold, first to generate a platform to host cells in a more physiologically relevant environment (using physiologically relevant fluid shear stress (FSS)) and second to show efficient integration of multiple different methods for assessing cell morphology, differentiation, and integrity. These include optical imaging, impedance monitoring, metabolite sensing, and a wound-healing assay. We illustrate the versatility of this multi-parametric monitoring in giving us increased confidence to validate the improved differentiation of cells toward a physiological profile under FSS, thus yielding more accurate data when used to assess the effect of drugs or toxins. Overall, this platform will enable high-content screening for in vitro drug discovery and toxicology testing and bridges the existing gap in the integration of in-line sensors in microfluidic devices.

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Section: Discussionmentioning

confidence: 99%

Organic transistor platform with integrated microfluidics for in-line multi-parametric in vitro cell monitoring

et al. 2017

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“…The active surface area can be even further increased by fabrication of conductive polymer coated 3D scaffolds [68][69][70][71]. This is facilitated by simple fabrication techniques like electrodeposition or vapor phase deposition.…”

Section: Sensorsmentioning

confidence: 99%

Organic Bioelectronic Tools for Biomedical Applications

2015

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Abstract:Organic bioelectronics forms the basis of conductive polymer tools with great potential for application in biomedical science and medicine. It is a rapidly growing field of both academic and industrial interest since conductive polymers bridge the gap between electronics and biology by being electronically and ionically conductive. This feature can be employed in numerous ways by choosing the right polyelectrolyte system and tuning its properties towards the intended application. This review highlights how active organic bioelectronic surfaces can be used to control cell attachment and release as well as to trigger cell signaling by means of electrical, chemical or mechanical actuation. Furthermore, we report on the unique properties of conductive polymers that make them outstanding materials for labeled or label-free biosensors. Techniques for electronically controlled ion transport in organic bioelectronic devices are introduced, and examples are provided to illustrate their use in self-regulated medical devices. Organic bioelectronics have great potential to become a primary platform in future bioelectronics. We therefore introduce current applications that will aid in the development of advanced in vitro systems for biomedical science and of automated systems for applications in neuroscience, cell biology and infection biology. Considering this broad spectrum of applications, organic bioelectronics could lead to timely detection of disease, and facilitate the use of remote and personalized medicine. As such, organic bioelectronics might contribute to efficient healthcare and reduced hospitalization times for patients. OPEN ACCESSElectronics 2015, 4 880

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“…In an effort to address this important unmet medical need research has focused on tissue engineering/regenerative medicine approaches, specifically the use of acellular extracellular matrix (ECM) scaffolds, seeded with myogenic cell sources for subsequent implantation [11][12][13]. Other approaches have employed rationally engineered biomaterial platforms that provide chemical and physical cues to direct cell survival and more accurately mimic healthy ECM microenvironments [14][15][16][17]. Numerous studies also combine biomaterials with isolated muscle cells as well as supporting cells including neurons, endothelial cells, and fibroblasts to augment the repair process [11,[18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, collagenglycosaminoglycan (CG) scaffolds produced via freeze-drying have previously been applied across a wide range of tissue engineering applications including bone [46], cardiac tissue [47], and peripheral nerves [48], and have also been used clinically in the regeneration of skin [49,50]. An additional benefit of this system is that material properties can be easily controlled by altering processing parameters such as freezing and lyophilization rate as well as precursor suspension composition [16,51,52]. As a result, this system is ideal for the facile introduction of conductive polymers into the bulk suspension prior to lyophilization to produce 3D conductive biomaterial composites.…”

Section: Introductionmentioning

confidence: 99%

Aligned and Conductive 3D Collagen Scaffolds for Skeletal Muscle Tissue Engineering

et al. 2020

Preprint

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Skeletal muscle is characterized by its three-dimensional (3D) anisotropic architecture composed of highly aligned, organized, and electrically excitable muscle fibers that enable normal locomotion. Biomaterial-based tissue engineering approaches to repair skeletal muscle injuries are limited due to difficulties in combining 3D structural alignment (to guide cell/matrix organization) and electrical conductivity (to enable electrically excitable myotube assembly and maturation). In this work we successfully produced aligned and electrically conductive 3D collagen scaffolds using a freeze-drying approach. Conductive polypyrrole (PPy) microparticles were synthesized and directly mixed into a suspension of type I collagen and chondroitin sulfate followed by directional lyophilization. Scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and confocal microscopy analyses showed that directional solidification resulted in scaffolds with longitudinally aligned macropores (transverse plane: 155 ± 27 µm, longitudinal plane: 218 ± 49 µm) with homogeneously-distributed PPy content. Chronopotentiometry verified that PPy incorporation resulted in a five-fold increase in conductivity when compared to non-PPy containing collagen scaffolds without detrimentally affecting C2C12 mouse myoblast metabolic activity. Furthermore, the aligned scaffold microstructure provided contact guidance cues that directed myoblast growth and organization. Incorporation of PPy also promoted enhanced myotube formation and maturation as measured by myosin heavy chain (MHC) expression and number of nuclei per myotube. Together these data suggest that aligned and conductive 3D collagen scaffolds could be useful for skeletal muscle tissue engineering.

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3D conducting polymer platforms for electrical control of protein conformation and cellular functions

Cited by 116 publications

References 59 publications

Organic transistor platform with integrated microfluidics for in-line multi-parametric in vitro cell monitoring

Organic transistor platform with integrated microfluidics for in-line multi-parametric in vitro cell monitoring

Organic Bioelectronic Tools for Biomedical Applications

Aligned and Conductive 3D Collagen Scaffolds for Skeletal Muscle Tissue Engineering

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