Electrical conductivity test for evaluating physiological quality in snap bean (Phaseolus vulgaris L.) seeds

Abstract

Snap bean is a variety of common bean (Phaseolus vulgaris L.) that is cultivated and consumed as a vegetable worldwide. In order to optimize the crop's production, germination tests are used to ensure that seeds are high in quality. However, because these tests possess inherent limitations, vigor tests, such as the electrical conductivity test, may be used instead to assess seed quality. The goal of the present study was to develop a standardized methodology for measuring electrical conductivity in snap bean seed that could reliably assess the physiological quality of seed batches. Groups of seeds (n = 50) from each of seven snap bean genotypes (UEL 1, UEL 2, HAV 21, HAV 22, HAV 34, HAV 35, and HAV 42) were immersed in distilled water (75 or 150 mL) for various immersion periods (2, 4, 8, 12, 16, 20, or 24 h), using a completely randomized 7 × 2 × 7 factorial design, with eight replicates per treatment. After immersion, the electrical conductivity of seeds from each treatment group was measured in μS cm-1 using a conductivity meter. The group means were subjected to ANOVA and the Scott-Knott test (p ≤ 0.05). The optimal conditions for the electrical conductivity test in snap bean seeds were a 16-h immersion in 75 mL of water. Under these conditions, genotypes could be clearly differentiated based on their seed vigor, optimizing the measurement of seed quality.

Advancing horizons in vegetable cultivation: a journey from ageold practices to high-tech greenhouse cultivation—a review

“…Additional methodologies, like electrical conductivity tests, offer unique insights into seed vigor by quantifying electrolyte leakage providing a proxy for membrane integrity and overall seed health ( Ortiz et al., 2018 ). Pathogen detection has also seen significant advancements with techniques, such as PCR coupled with nanomaterial-based sensors, enabling rapid, accurate identification of seed-borne pathogens ensuring the sowing of healthy, disease-free seeds ( Kumar et al., 2021 ).…”

Mitigasi Cekaman Salinitas pada Fase Perkecambahan Kedelai melalui Invigorasi dengan Ekstrak Kulit Manggis dan Ekstrak Kunyit

“…Bobot kering kecambah didapat setelah kecambah dioven pada suhu 100 0 C, hingga bobotna konstan. Data daya hantar listrik diperoleh dengan cara merendam benih selama 24 jam, lalu air rendamannya diukur dengan Conductivity Meter (Ortiz et al, 2018).…”

“…Keluarnya elektrolit dari dalam sel mengindikasikan tingkat kerusakan atau disorganisasi membran sel, sekaligus juga mencerminkan kondisi kualitas fisiologis dan tingkat vigor benih yang bersangkutan. Hal ini sejalan dengan pendapat Ortiz et al (2018) yang menyatakan bahwa uji daya hantar listrik berhubungan langsung dengan vigor benih dan integritas membran sel benih. Cekaman salinitas memicu produksi ROS secara berlebihan yang melebihi sistem pertahanan antioksidan (Ahmad et al 2019).…”

Electrical Conductivity and Quality of Different Umbel Order Carrot Seeds

“…Vigor tests are used in conjunction to germination tests, to support decisions, increase agility and effi ciency of quality control (Bittencourt et al;. Amongst the fastest tests studied, the electrical conductivity is related with the initial events of the deterioration sequence in seeds (Bewley & Black, 1994;Ortiz, et al, 2018). The results are obtained through indirect evaluation of the structural degree of the cell membranes by means of determining the quantity of leached ions in the imbibing solution (Vieira & Krzyzanowski, 1999;Marcos Filho, 2015).…”