Electric Cigarettes’s Effect to the Mda Levels in Blood of Wistar Rat

Rambung, Etha

doi:10.32883/hcj.v5i1.636

Cited by 3 publications

(3 citation statements)

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“…Both conventional cigarette smoke and electronic cigarettes were known to cause an increase in reactive oxygen species (ROS), subsequently leading to oxidative stress development (Omolaoye, El Shahawy, Skosana, Boillat, Loney, & Du Plessis, 2022;Rambung, 2020). Increased reactive oxygen species (ROS) caused cellular, tissue, or organ damage due to lactate dehydrogenase activity, increased oxidative stress markers (lipid peroxidation, protein carbonylation), and reduced antioxidant enzyme activity (Tooy, Tendean, & Satiawati, 2016;Vivarelli, Canistro, Cirillo, Cardenia, Rodriguez-Estrada, & Paolini, 2019).…”

Section: Discussionmentioning

confidence: 99%

Differences in diameter and thickness of the seminiferous tubules of male wistar rats (rattus norvegicus) after being exposed to e-cigarette smoke and conventional cigarettes

Iring,

Rambung,

Winarso

2023

Internat.Jrnl.

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Background: Electric cigarettes and conventional cigarettes have a detrimental effect on their users. Cigarettes contain ingredients that reduce the quality of male reproduction. Purpose: To determine the differences in diameter and thickness of the seminiferous tubules of male Wistar rats (rattus norvegicus) exposed to electric cigarettes compared to conventional cigarettes. Method: This research is a true experimental research in the laboratory using a post test-only control group design. The treatment group consisted of group K- as the negative control group, group K+ which was included in the smoking chamber but not exposed to cigarette smoke, group P1 which was included in the smoking chamber and exposed to electric cigarette smoke, group P2 which was included in the smoking chamber and exposed to conventional cigarette smoke. The treatment was carried out for 30 days and then testicular samples were taken to calculate diameter and thickness of the seminiferous tubules. Data were analyzed with SPSS using descriptive statistics and One Way ANOVA and Kruskall Wall is tests. Results: There was a decrease in the diameter and thickness of the seminiferous tubules in the treatment group which was significantly different from the control group. The results of the LSD post hoc test for differences in seminiferous tubule diameter between groups P1 and P2 were p=0.72 > 0.05. The results of the Mann Whitney test showed that the difference in seminiferous tubule thickness between groups P1 and P2 was p=0.06 > 0.05.Conclusion: Exposure to electric and conventional cigarettes caused changes in the diameter and thickness of the seminiferous tubules from normal conditions. There was no significant difference in the impact of exposure to electric and conventional cigarettes, maybe because the damage they produced was not different.

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Section: Discussionmentioning

confidence: 99%

Differences in diameter and thickness of the seminiferous tubules of male wistar rats (rattus norvegicus) after being exposed to e-cigarette smoke and conventional cigarettes

Iring,

Rambung,

Winarso

2023

Internat.Jrnl.

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“…Hal ini menunjukkan bahwa pemaparan rokok elektrik menyebabkan kerusakan dan kematian inti sel purkinje sehingga mengakibatkan penurunan jumlah sel purkinje serebelum tikus wistar jantan. Kerusakan inti sel ini mungkin dipicu oleh stress oksidatif dan peroksidasi lipid akibat paparan rokok elektrik 7 .…”

Section: Pembahasanunclassified

“…Rokok elektrik dapat menyebabkan stress oksidatif 7 . Hal ini disebabkan karena rokok elektrik menghasilkan reactive oxygen species (ROS) dan bersifat sitotoksik sehingga dapat menyebabkan kematian sel 8 .…”

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Perubahan Jumlah Sel Purkinje Serebelum Tikus Wistar Jantan Setelah Pemaparan Rokok Elektrik

Rambung

2023

eum

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Design, taste, marketing and perceptions of safety from e-cigarettes have increased the appeal of e-cigarettes to young people around the world so that their use continues to increase. This is one of the threats in efforts to reduce the prevalence of smokers aged 10-18 years in Indonesia. Electric cigarettes cause oxidative stress while the brain is vulnerable to oxidative stress because it requires large amounts of oxygen. This experimental study with a posttest only control design aimed to determine changes in histological structure and number of male Wistar rat cerebellum Purkinje cells after exposure to electric cigarettes. This study used a sample of 32 male wistar rats (Rattus norvegicus) which were divided into 4 treatment groups: group A (without treatment), group B (put in an exposure cage), group C (given exposure to electric cigarettes 15 times in a cage exposure), and group D (given exposure to e-cigarettes 30 times in the exposure cage). Exposure to e-cigarettes was given for 50 days, then sacrificed and samples were made with Hematoxylin Eosyn (HE) staining. The results showed that the number of cerebellar purkinje cells in male Wistar rats exposed to electric cigarettes decreased significantly and there was a significant difference in the number of cells between 15 and 30 exposures and karyolysis occurred in the cells. E-cigarettes cause core damage and decrease in the number of Purkinje cells in the cerebellum of male wistar rats.

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Electronic Cigarettes Versus Tobacco Poly Use: Reduced Oxidative Stress but Similar Inflammatory Effect

Khan

Al-Najar

Tabassum

et al. 2023

NEMJ

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Background: Smoking in any form is a significant risk factor for developing cardiovascular diseases, chronic obstructive pulmonary disease, lung cancer, and other oxidative damage-induced diseases. The increasing global trend of E-cigarettes has led traditional smokers to perceive them as a safer option. background: Smoking in any form is a significant risk factor in developing cardiovascular diseases, chronic obstructive pulmonary disease, lung cancer, and oxidative damage. The global trend in E-cigarettes has led traditional smokers to believe it to be the safer option as it does not burn tobacco. Objectives: This study aimed to compare the levels of malondialdehyde (MDA) as a marker of oxidative damage and high-sensitivity C-reactive protein (hs-CRP) as a marker of inflammation between pure E-cigarette users and tobacco poly-users (smoking combinations of traditional products). Moreover, it also evaluated the influence of Body Mass Index, frequency, and duration of vaping/smoking on these biomarkers. objective: This study aimed to compare the differences in the levels of Malondialdehyde (MDA) as a marker of oxidative damage and high sensitivity C-reactive protein (hs-CRP) as a marker of inflammation between Pure E-cigarette users and polyusers who smoke combinations of different types of tobacco. Further, observe the influence of Body Mass Index, frequency, and duration of vaping/smoking on the biomarkers between the two groups. Materials and Methods: This analytical cross-sectional pilot study included 40 males aged 18-25 (20 pure E-cigarette users and 20 tobacco poly-users). MDA and hs-CRP were estimated on serum, and SPSS-Version 28.0.1.1 was used for data analysis. Mean hs-CRP and MDA levels for the two groups were compared using the Mann-Whitney-U test. method: This analytical cross-sectional pilot study was conducted with 40 males aged 18-25 (20 Pure E-cigarette users and 20 tobacco polyusers). MDA and hs-CRP were estimated on serum using standard procedures. SPSS Version 28.0.1.1 was used for data analysis. Mean hs-CRP and MDA levels were recorded for the two groups, and a comparison was made using the Mann-Whitney test. Results: Mean level of hs-CRP between the two groups was not significantly different. However, MDA levels were lower in pure E-cigarette users than in tobacco poly-users, especially among those with normal BMI and those who vaped/smoked more frequently and for a longer duration result: The mean level of hs-CRP between both groups was not significantly different; however, MDA levels were lower in Pure E-cigarette users than in tobacco polyusers, especially among those with normal BMI, those who vaped/smoked more frequently, and those who have been vaping/smoking for years. Conclusion: Oxidative damage was lesser for pure E-cigarette users and could potentially be the less harmful option than tobacco poly-use. However, E-cigarettes are not the safest substitute for conventional smoking as it causes a similar extent of risk for inflammation-related CVD. Findings need further exploration to study the long-term effects on a larger population group to draw definitive conclusions. conclusion: Oxidative damage was lesser for pure E-cigarette users and could potentially be the less harmful option than tobacco polyuse. However, E-cigarettes are not the safest substitute for conventional smoking as it causes a similar extent of risk for inflammation-related diseases. The only effort needed for a healthy lifestyle is to target smoking cessation rather than substitution.

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Electric Cigarettes’s Effect to the Mda Levels in Blood of Wistar Rat

Cited by 3 publications

References 10 publications

Differences in diameter and thickness of the seminiferous tubules of male wistar rats (rattus norvegicus) after being exposed to e-cigarette smoke and conventional cigarettes

Differences in diameter and thickness of the seminiferous tubules of male wistar rats (rattus norvegicus) after being exposed to e-cigarette smoke and conventional cigarettes

Perubahan Jumlah Sel Purkinje Serebelum Tikus Wistar Jantan Setelah Pemaparan Rokok Elektrik

Electronic Cigarettes Versus Tobacco Poly Use: Reduced Oxidative Stress but Similar Inflammatory Effect

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