Ekstrakharuan(Channa striata)secara efektif menurunkan jumlah limfosit fase inflamasi dalam penyembuhan luka (Extract of haruan (Channa striata) decreases lymphocyte count in inflammatory phase of wound healing process effectively)

Abstract

Haruan is river-water fish usually consumed by South Kalimantan people. Haruan extract contains albumin, Zn, Fe,Cu and unsaturated fatty acid which can act as antiinflammatory substances and accelarate wound healing. The aim ofthis study is to examine effectiveness of haruan extract on lymphocyte count in infammatory phase of wound healingprocess and compare it to ibuprofen. This study used true experimental method with post-test only control group designand simple random sampling. Samples were divided into 5 treatment groups:which were given the extract for 7 sevendays: 25% haruan extract group, 50% haruan extract group, 100% haruan extract group, negative control K(-) usingaquadest, positive control K(+) given ibuprofen suspension with dosage of 20mg/kg weight/day. Lymphocyte cells wereobserved using microscope and counted per field of view. Lymphocyte count reached its peak on day 5. Lymphocytecount means in each group were 4,2 ± 0,566; 1± 0,000; 3,8 ± 2,546; 7,1 ± 0,141; dan 7,5 ± 3,818 respectively. Two way ANOVA and post hoc LSD tests showed that lymphocyte count significantly decreased in 50% and 100% treatmentgroups (p<0,05) and has equal effect with ibuprofen in decreasing lymphocyte count. This study concluded that haruanextract significantly decreased lymphocyte count in wound healing process.

Aloe vera Extract Sheet and Egg Albumin (Gallus domesticus) Reduce the Number of Lymphocytes on Gingival Incision Wound

“…The activated macrophages will release cytokines, such as interleukin-1 (IL-1) and tumor necrosis factor (TNF), which will activate lymphocytes. Macrophages and lymphocytes stimulate each other to eliminate triggering antigenic agents so that fibroblasts form a strong tissue in the proliferative phase (10). The decrease of lymphocytes on the 7th day occurs because the existing inflammatory cells will undergo apoptosis therefore their duties as phagocytic agents are replaced by fibroblasts which form new tissue in the proliferative phase (19).…”

“…The subtraction of expression of MMP-8 in tissues may be associated with decreased inflammation (28). Izzaty et al, state that the content of albumin in the extract of tilapia fish could reduce the number of inflammatory phase lymphocytes in the healing process of the buccal mucosa of Wistar rats (10).…”

“…The number of lymphocytes peaked on the 3rd to 6th day and decreased on the 7th day. Lymphocytes together with macrophages have a role to stimulate each other to eliminate triggering antigenic agents so fibroblasts form a strong tissue in the proliferative phase (10). Aloe vera contains active compounds that are potent antiinflammatory agents and can accelerate the woundhealing process (11).…”

An Effect of Giving Snakehead Fish Extract ( Channa striata ) on Albumin, Neutrophil, and Lymphocyte Levels in Hypoalbuminemia Patients

“…Research by Izzaty, et al (2014) showed that the extract of haruan or snakehead fish could reduce the number of inflammatory phase lymphocytes in the healing process of the buccal mucosa of wistar rats with the best concentration of 50%. The content of albumin, Zn, Fe, Cu and unsaturated fatty acids are useful in reducing inflammation.…”

The effect of the pseudostem extract of ambon banana on the number of lymphocytes and fibroblasts in gingivitis : an experimental study

“…The increase in lymphocytes must be kept to a minimum to avoid long-term inflammation that can cause wound-healing complications. 5 In gingivitis, tissue damage is detected not only in the epithelial cells but also in the surrounding matrix. Therefore, restoring the epithelial mass and its normal structural framework is necessary.…”