Ekstrak Daun Salam Dalam Meningkatkan Ekspresi Fibroblast Growth Factor Pada Ulkus Traumatik Rongga Mulut

Abstract

Background: Traumatic ulcers are lesions of the oral mucosa caused by mechanical, chemical, and temperature trauma. Traumatic ulcer treatment aims to reduce inflammation, accelerate the healing process, and reduce pain. Bay leaf has anti-inflammatory pharmacological activities. This study aims to determine the effect of bay leaf extract on the expression of fibroblast growth factor in traumatic ulcer oral mucosa in Wistar rats. Method: This is an experimental research with a post-test only control group design, consisting of four groups with 20 sample of male Wistar rats. All animals were injured using a hot burnisher attached to the labial mucosa for 1 second. The gel was applied twice daily until the 3rd and 5th day after the ulcer has formed. Rat mucosal tissues were analyzed by immunohistochemical staining for FGF expressions. Statistical analysis using SPSS Mann-Whitney test. Result: The results showed the mean of cells expressing FGF in the bay gel extract group on the 3rd and 5th days were 8.4 ± 0.8, and 13.6 ± 0.5. Mann Whitney test results showed P<0.05 there is a significant differential expression of FGF traumatic ulcer oral mucosa of experimental animals on days 3 and 5 compared control group. Conclusion: Bay leaf extract increased FGF expression on days 3 and 5 PENDAHULUANUlkus rongga mulut adalah peradangan pada bagian mukosa yang disebabkan kerusakan jaringan epitel rongga mulut dan terbukanya ujung saraf. Traumatik berkaitan adanya trauma mekanik, kimia, dan suhu. Gambaran klinis dari ulkus traumatik merupakan lesi sekunder dengan gambaran khas berupa bentuk soliter bulat, berukuran variatif, kedalaman lesi mencapai lamina propia 1,2,3 .Proses penyembuhan ulkus traumatik dibagi menjadi 3 tahap, fase inflamasi, proliferasi, dan remodeling. Fase inflamasi dibagi menjadi 2 yaitu fase inflamasi awal dan fase inflamasi akhir 4 . Sel yang berperan penting adalah makrofag. Makrofag diklasifikasikan menjadi 2 yaitu makrofag proinflamasi yang berperan pada fase inflamasi awal dan makrofag antiinflamasi atau pro-angiogenik yang berperan pada fase inflamasi akhir.

Comparative analysis of KnockOut serum replacement and fetal bovine serum for in vitro culture of human dermal fibroblasts

“…Fetal bovine serum and KOSR have vitamins/antioxidants and proteins that can increase FGF mRNA relative expression, leading to increased proliferation, differentiation, and migration of fibroblasts that contribute to a faster wound-healing process. (15) The presence of serum in cell culture constitutes an extracellular stimulatory process that uses intracellular signals via the Rho-actin/myocardin-related transcription factor (MRTF) and Ras ERK/ternary complex factor (TCF) pathways. These factors act as a cofactor for serum response factor (SRF) in the nucleus to regulate cell differentiation, migration, motility, proliferation, cytoskeleton, signaling, adhesion, development, myogenesis, apoptosis, etc.…”

Bay Leaf (Syzygium Polyanthum) Extract Effect on IL-10 and IL-6 Gene Expression in Traumatic Ulcer

“…The results of a study concluded that 10% bay leaf extract in a cream preparation had an inflammatory effect on carrageenin-induced rat paw edema 10. In another study, 10% bay leaf extract gel was found to affect several inflammatory mediators, such as decreasing TNF-α gene expression and increasing IL-10 and FGF gene expression [15][16][17] . However, there are yet to be many studies that prove the effect of bay leaves on traumatic ulcers, especially on IL-6 and IL-10 gene expression.…”

<p>Efektivitas getah batang betadine (Jatropha multifida L.) terhadap penyembuhan luka pasca pencabutan gigi pada tikus Sprague-Dawley</p><p>Effectiveness of betadine (Jatropha multifida L.) stem sap on the wound healing after tooth extraction in Sprague-Dawley rats<p>

“…Salah satu bentuk aplikasi pengobatan dengan tanaman obat adalah pengobatan pada luka yang sering dilakukan oleh masyarakat. 4 Salah satu tanaman yang digunakan oleh masyarakat untuk mengobati luka adalah tanaman Jatropha multifida L. 5 Jatropha multifida L. dikenal sebagai tanaman betadine. 3 Tanaman betadine oleh banyak masyarakat Sumatera Selatan digunakan sebagai tanaman hias, sebagian masyarakat ada yang menggunakan sebagai tanaman untuk obat luka.…”