“…It was not possible to detect mono-or diacyl-glycerols. The amount of triacylglycerols were determined by enzymic glycerol analysis [4] of hydrolyzed samples of total lipid, ignoring the small amounts of glycerol from phospholipid. Sterol esters were estimated by sterol analysis [5] of lipid eluted from TLC spots running as ergesterol ester and hydrolyzed.…”
“…It was not possible to detect mono-or diacyl-glycerols. The amount of triacylglycerols were determined by enzymic glycerol analysis [4] of hydrolyzed samples of total lipid, ignoring the small amounts of glycerol from phospholipid. Sterol esters were estimated by sterol analysis [5] of lipid eluted from TLC spots running as ergesterol ester and hydrolyzed.…”
“…Non-esterified fatty acids were measured according to Trout's f 121 modi~cation of Dole's titration method [ 131. Glycerol was determined according to Eggstein and Kreutz [14] with a test set of Boehringer, Mannheim.…”
“…Serum FFA were determined after Duncombe et al [6] and aspartate aminotransferase (ASAT) (EC 2.6.1.1) activity according to Ceriotti [7] in blood collected after decapitation. Liver was quickly excised after decapitation and TAG determined by the method of Eggstein et al [8].…”
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