Effects of volatile fatty acids, ketone bodies, glucose, and insulin on lipolysis in bovine adipose tissue

Metz, S.H.M.; Bergh, S.G. van den

doi:10.1016/0014-5793(72)80137-6

Cited by 36 publications

(11 citation statements)

References 18 publications

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“…M, respectively [1]. The in vitro maximal activity of T was 40 and 69 % of the activity of ISO, in pigs [35] [36], but this seems to be unlikely in our study because the maximal concentrations of (3-OH (0.34-0.44 mmot-L-1 ) were much lower than those (2-4 mmol-L-1 ) required for an antilipolytic effect.…”

Section: Animals and Dietsmentioning

confidence: 41%

Effects of the infusion of non-selective β-, and selective β1- or β2-adrenergic agonists, on body fat mobilisation in underfed or overfed non-pregnant heifers

Ferlay

Chilliard²

1999

Reprod. Nutr. Dev.

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-The aim of this study was to analyse the effects of the intravenous infusion of a non-selective p-agonist (j3-A) (isoproterenol, ISO), and selective j31-A (dobutamine, D) or p2-A (terbutaline, T) on body fat mobilisation in non-pregnant heifers, in a 2 x 2 crossover design with two treatments (underfeeding or overfeeding) and two periods. The effect of the duration of submission to each energy level on basal and ISO-induced fat mobilisation was also studied. ISO

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Section: Animals and Dietsmentioning

confidence: 41%

Effects of the infusion of non-selective β-, and selective β1- or β2-adrenergic agonists, on body fat mobilisation in underfed or overfed non-pregnant heifers

Ferlay

Chilliard²

1999

Reprod. Nutr. Dev.

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“…A study in mouse adipocytes found that BHBA at a concentration of 2 and 20 mM, but not at 0.2 mM, reduced isoproterenol-induced NEFA release (Taggart et al, 2005). In noradrenaline-stimulated bovine SCAT, a 10 mM concentration of BHBA (Metz and van den Bergh, 1972) and 5 mM concentration of BHBA (Rukkwamsuk et al, 1998) was also shown to decrease NEFA or glycerol release in vitro.…”

Section: Antilipolytic Responsementioning

confidence: 96%

Agonists of the G protein-coupled receptor 109A-mediated pathway promote antilipolysis by reducing serine residue 563 phosphorylation of hormone-sensitive lipase in bovine adipose tissue explants

Kenéz

Locher

Rehage

et al. 2014

Journal of Dairy Science

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A balanced lipolytic regulation in adipose tissues based on fine-tuning of prolipolytic and antilipolytic pathways is of vital importance to maintain the metabolic health in dairy cows. Antilipolytic pathways, such as the G protein-coupled receptor 109A (GPR109A)-mediated pathway and the insulin signaling pathway in bovine adipose tissues may be involved in prohibiting excessive lipomobilization by reducing triglycerol hydrolysis. This study aimed to evaluate the in vitro antilipolytic potential of the mentioned pathways in bovine adipose tissue explants. Therefore, subcutaneous and retroperitoneal adipose tissue samples (approximately 100mg) of German Holstein cows were treated for 90 min ex vivo with nicotinic acid (2, 8, or 32 μM), nicotinamide (2, 8, or 32 μM), β-hydroxybutyrate (0.2, 1, or 5mM), or insulin (12 mU/L), with a concurrent lipolytic challenge provoked with 1 μM isoproterenol. Lipolytic and antilipolytic responses of the adipose tissues were assessed by measuring free glycerol and nonesterified fatty acid release. To identify molecular components of the investigated antilipolytic pathways, protein abundance of GPR109A and the extent of hormone-sensitive lipase (HSL) phosphorylation at serine residue 563 were detected by Western blotting. Treatment with nicotinic acid or β-hydroxybutyrate decreased the lipolytic response in adipose tissue explants and concurrently reduced the extent of HSL phosphorylation, but treatment with nicotinamide or insulin did not. Subcutaneous adipose tissue constitutively expressed more GPR109A protein, but no other depot-specific differences were observed. This study provides evidence that the GPR109A-mediated pathway is functionally existent in bovine adipose tissues, and confirms that HSL phosphorylation at serine residue 563 is also important in antilipolytic regulation in vitro. This antilipolytic pathway may be involved in a balanced lipid mobilization in the dairy cow.

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“…Une tendance à la lipomobilisation a toutefois été observée dans certaines expériences de longue durée (Soderholm et al, 1986a, b Emery, 1973 ;Convey, 1974 ;Patterson et Linzell, 1974 ;Bauman, 1976 ;Swan, 1976 ;Coggins et Field, 1978 ;Hart et al, 1978Hart et al, , 1979Miller et al, 1978 ;Cowie et al, 1980 ;Walsh et al, 1980 ;Trenkle, 1981 ;Blum et al, 1982Blum et al, , 1983Blum, 1983, Mc Dowell, 1983Bitman et al, 1984b ;Collier et al, 1984 ;Refsal et al, 1984 ;Aceves et al, 1985 ;Akasha et al, 1985 ;Frohli et Pethes et al, 1985 ;Plaut et al, 1985 ;Thilsted, 1985b ;Bauman et Mc Cutcheon, 1986 ;Gerloff et al, 1986bJ. Les réponses de la sécrétion d'hormone de croissance à la sous-alimentation (revue de Vernon, 1980) ou à la gestation (Koprowski et Tucker, 1973) (Collier et al, 1984). La mobilisation des protéines corporelles (revue de Chilliard et al, 1983) Van den Bergh, 1972 ;Metz et al, 1974 (Little, 1974 ;Rowlands, 1978 ;Treacher, 1978 ;Larnicol et al, 1984). Le rapport « AGNE mobilisés/glycérol mobilisé » est supérieur à 3 (Yang et Baldwin, 1973a ;…”

Section: Préambuleunclassified

Revue bibliographique : Variations quantitatives et métabolisme des lipides dans les tissus adipeux et le foie au cours du cycle gestation-lactation 2e partie : chez la brebis et la vache

Chilliard¹

1987

Reprod. Nutr. Dévelop.

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(cf. I.N.R.A., 1978) Par rapport à la ratte, qui a fait l'objet de la première partie de cette revue bibliographique (Chilliard, 1986), les ruminants présentent des particularités digestives qui conditionnent largement, par la nature des nutriments absorbés, l'orientation de leur métabolisme. En effet, les aliments qu'ils ingèrent, riches en parois végétales, séjournent pendant 15 à 48 h dans le rumen-réseau (dont le contenu est de 60 à 100 I chez le bovin adulte), où ils subissent une dégradation plus ou moins complète sous l'action des enzymes sécrétées par les micro-organismes qui y pullulent (10 10 par ml).Les (Basset, 1975). Le métabolisme des lipides dans le foie de ruminant a été décrit par Rémésy et al. (1984, 1986 Ballard, 1967Ballard et al., 1969Ballard et al., , 1972Hood et a/., 1972 ; Ingle et al., 1972a, b ;Vézinhet, 1976 ;Liepa et al., 1978 ;Prior, 1978 ;Smith et Crouse, 1984

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Effects of volatile fatty acids, ketone bodies, glucose, and insulin on lipolysis in bovine adipose tissue

Cited by 36 publications

References 18 publications

Effects of the infusion of non-selective β-, and selective β1- or β2-adrenergic agonists, on body fat mobilisation in underfed or overfed non-pregnant heifers

Effects of the infusion of non-selective β-, and selective β1- or β2-adrenergic agonists, on body fat mobilisation in underfed or overfed non-pregnant heifers

Agonists of the G protein-coupled receptor 109A-mediated pathway promote antilipolysis by reducing serine residue 563 phosphorylation of hormone-sensitive lipase in bovine adipose tissue explants

Revue bibliographique : Variations quantitatives et métabolisme des lipides dans les tissus adipeux et le foie au cours du cycle gestation-lactation 2e partie : chez la brebis et la vache

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