Egg yolk-derived immunoglobulin (IgY) against Porphyromonas gingivalis 40-kDa outer membrane protein inhibits coaggregation activity

Hamajima, Susumu; Maruyama, Mitsuhiro; Hijiya, Takahiro; Hatta, Hajime; Abiko, Yoshimitsu

doi:10.1016/j.archoralbio.2006.12.013

Cited by 25 publications

(21 citation statements)

References 36 publications

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“…The ability of IgY specific to bind to the pathogenic bacteria will change the bacteria surface that can weaken the bacteria to attach (adhere) to the host cell. Hamajima et al (2007) mentions that specific IgY can bind to the outer membrane proteins (outer membrane protein / OMP) of P. gingivalis bacteria that inhibit these bacteria to conduct co-aggregation. Other mechanism mentioned that a high IgY antibody titer will have great purity and effectively to neutralize various antigens in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Effects of immunoglobulin Y (IgY) serum against plaque bacteria

Handajani¹,

Narissi²,

Azis³

et al. 2016

Afr. J. Microbiol. Res.

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Dental caries is an infectious disease caused by plaque bacteria, especially Streptoccus mutans. Many studies were conducted to develop methods or material for dental caries prevention. It was using passive immunization that will produce specific antibodies. Yolk immunoglobulin (IgY) is antibody in the serum and egg yolk. This study aimed to determine the effect of IgY serum against the growth of dental plaque bacterial in vitro. In the long term, it is expected IgY specific S. mutans can be as one of material could prevent dental caries topically. Immunization in the chicken was performed using S. mutans antigen. Immunoglobulin Y (IgY) specific S. mutans was collected from blood serum of Hysex Brown chicken. Antibacterial effect of IgY serum on the growth of bacteria that cause dental plaque (Streptococcus alpha, Staphylococcus aureus, and S. mutans) was done using diffusion method. Sample was performed on 10 plate each bacteria. Data inhibition zone were analyzed using ANOVA and LSD. There was no inhibition zone of IgY serum on the growth of S. alpha and S. aureus while positive results obtained against S. mutans. Inhibition zone increased according increasing the concentration of IgY. ANOVA test showed significant differences the effect of concentration IgY serum on the inhibition zones of S. mutans (p <0.05). A significant difference (p<0.05) from the LSD was obtained in the comparison between the concentration of IgY, IgY serum and controls. In conclusion, IgY serum that immunized S. mutans has antibacterial effect against S. mutans only.

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Section: Discussionmentioning

confidence: 99%

Effects of immunoglobulin Y (IgY) serum against plaque bacteria

Handajani¹,

Narissi²,

Azis³

et al. 2016

Afr. J. Microbiol. Res.

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“…Oral administration of IgY antibodies has been tested for many years with promising results [80] to different pathogens as human rotavirus [81]; dental plaque formation by Streptococcus mutans [82,83]; enteropathogenic E. coli [84]; Helicobacter pylori [85,86]; Cryptosporidium parvum [87,88]; canine parvovirus [89]; Porphyromonas gingivalis [90]; Pseudomonas aeruginosa [91]; shrimp's white spot syndrome virus [92]; Eimeria acervulina [93]; E. tenella and E. maxima [94,95]; H5N1 e H1N1 in mice [96]; Vibrio cholerae [97]; rotavirus and norovirus [98]; Campylobacter jejuni [99][100][101]; and botulinum neurotoxins [102]. Immunotherapy as a passive immunization method to neutralize venom using purified IgY proved to be efficient for therapy protocol [103][104][105][106][107].…”

Section: Using Igy For Passive Immunizationmentioning

confidence: 99%

IgY-Technology Applied to Studies of Toxoplasma gondii Infection

Júnior¹,

Santos²,

Bassi³

et al. 2017

Toxoplasmosis

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In this chapter, we describe relevant aspects of immunoglobulin Y (IgY) technology for Toxoplasma gondii applications, including comparison of avian IgY antibody with mammalian IgG antibody, egg yolk IgY production and isolation procedures, important applications for IgY antibody, and state of the art and perspectives for IgY-technology in T. gondii studies. T. gondii is a worldwide public health problem. IgY-technology provides an alternative antibody (IgY) to mammalian Immunoglobulin G (IgG) antibody. IgY-technology involves the chicken immunization, yolk IgY isolation, antibody characterization, and purified IgY application to several kinds of methods. Immunized chicken transfers a specific IgY from blood to egg yolk. Phylogenetic distance between chickens and mammals influences the generation of antibody repertoires recognizing an antigen profile. IgY is not bound to rheumatoid factor or mammalian complement protein and thus avoids the false-positive results. Yolk IgY isolation is carried out by simple procedures that are accessible for any laboratory and, also, for IgY isolation at large-scale production. IgY-technology provides antibodies for proteomic studies, diagnostic assays, and immunotherapy. Although IgY-technology is promising, there is a reduced number of investigations with IgY and T. gondii. Future perspectives involve the use of IgY-technology for the screening of new T. gondii antigens for diagnostics, therapy, or vaccine, development of innovative techniques for toxoplasmosis diagnostics and may be an immunotherapy for toxoplasmosis.

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“…The confirmation test of the stability of gel production involved the immunoreactivity of IgY, which was checked after storage for 30 days. The enzyme-linked immunosorbent assay (ELISA) technique was used to determine the lowest concentrations of anti-S. mutans IgY that still showed immunoreactivity against S. mutans [23].…”

Section: Preparation Of Igy Gelmentioning

confidence: 99%

Effect of topical anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats’ tooth surface

Bachtiar¹,

Afdhal

Meidyawati³

et al. 2016

Acta Microbiologica Et Immunologica Hungarica

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This study aims to evaluate the effect of anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats' tooth surface. Sprague Dawley rats were exposed intraorally with S. mutans Xc and were fed a caries-inducing diet 2000. The 24 rats were divided into four groups: group A had their teeth coated with IgY gel; group B received sterilized water as a control; group C had their teeth coated with IgY gel starting on the 29 th day; and group D had their teeth coated with a gel without IgY. Plaque samples were swabbed from the anterior teeth for S. mutans colony quantification, and saliva was collected to measure immunoreactivity by enzyme-linked immunosorbent assay. The results indicated that the quantity of S. mutans in rats treated with IgY gel showed significant difference compared with the controls. After coating with IgY anti-S. mutans gel, the mean immunoreactivity in rat saliva was higher than that of the no treatment group. In conclusion, topical application with anti-S. mutans IgY gel reduced the quantity of S. mutans on the tooth surface.

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Egg yolk-derived immunoglobulin (IgY) against Porphyromonas gingivalis 40-kDa outer membrane protein inhibits coaggregation activity

Cited by 25 publications

References 36 publications

Effects of immunoglobulin Y (IgY) serum against plaque bacteria

Effects of immunoglobulin Y (IgY) serum against plaque bacteria

IgY-Technology Applied to Studies of Toxoplasma gondii Infection

Effect of topical anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats’ tooth surface

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