Background: Hyperglycemia is a condition when blood sugar level is higher than normal. Hyperglycemia is also one of diabetes mellitus (DM) symptoms. Hyperglycemia has a correlation with the occurrence of periodontal disease. Curcuma zedoaria oil is known to decrease concentration of serum glucose. Purpose: This study was aimed to determine the effects of Curcuma zedoaria oil on high blood sugar level and gingivitis in rats. Method: This study used twenty-five male Wistar rats, divided into two groups, namely the treatment group and the control group. In the treatment group, fifteen rats were divided into three subgroups (each of which was induced with 10 μl/ml, 30 μl/ml and 50 μl/ml of Curcuma zedoaria oil). The control group was consisted of ten rats, divided into two subgroups, as the positive control group (induced with 10 mg/kg of Glibenclamide) and the negative control group (induced with propylene glycol). Streptozotocin (STZ) (Naclai tesque, Kyoto Japan) with a dose of 40 mg/kg was used to create hyperglycemia condition in those rats. Gingivitis was then made by using silk ligature in those hyperglycemia rats. Silk ligature was twisted at the margin of gingiva anterior mandibular incisors for seven days. After the rats had gingivitis, Curcuma zedoaria oil, glibenclamide and propylene glycol were orally administered for seven days. Their gingivitis condition was observed, and their blood sugar level was measured before and after the induction of STZ and during the treatment. The data obtained were analyzed by using Manova. Result: There were significant differences of blood sugar levels between the treatment group before and after the administration of Curcuma zedoaria oil and the positive control group (p<0.05). Healthy gingiva was then found in the treatment group and the positive control group. Conclusion: Curcuma zedoaria oil can decrease blood sugar level and gingivitis.
Dental caries is an infectious disease caused by plaque bacteria, especially Streptoccus mutans. Many studies were conducted to develop methods or material for dental caries prevention. It was using passive immunization that will produce specific antibodies. Yolk immunoglobulin (IgY) is antibody in the serum and egg yolk. This study aimed to determine the effect of IgY serum against the growth of dental plaque bacterial in vitro. In the long term, it is expected IgY specific S. mutans can be as one of material could prevent dental caries topically. Immunization in the chicken was performed using S. mutans antigen. Immunoglobulin Y (IgY) specific S. mutans was collected from blood serum of Hysex Brown chicken. Antibacterial effect of IgY serum on the growth of bacteria that cause dental plaque (Streptococcus alpha, Staphylococcus aureus, and S. mutans) was done using diffusion method. Sample was performed on 10 plate each bacteria. Data inhibition zone were analyzed using ANOVA and LSD. There was no inhibition zone of IgY serum on the growth of S. alpha and S. aureus while positive results obtained against S. mutans. Inhibition zone increased according increasing the concentration of IgY. ANOVA test showed significant differences the effect of concentration IgY serum on the inhibition zones of S. mutans (p <0.05). A significant difference (p<0.05) from the LSD was obtained in the comparison between the concentration of IgY, IgY serum and controls. In conclusion, IgY serum that immunized S. mutans has antibacterial effect against S. mutans only.
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