Efficient separation of N-Alkylamides from Piper longum L. using off-line two-dimensional coupled with gradient high-speed counter-current chromatography

Zhao, Liang; Ali, Iftikhar; Yu, Jinqian; Cui, Li; Zhu, Hongtu; Ma, Wenya; Li, Ge

doi:10.1016/j.lwt.2020.110817

Cited by 4 publications

(3 citation statements)

References 38 publications

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“…Nowadays, an interesting post-extraction technique used for the isolation and purification of alkaloids is high-speed countercurrent chromatography (HSCCC). 68,69 This technique, based on the partition coefficient of the analyte between two immiscible solvent systems, showed a high separation efficiency with higher sample recovery (grams) and a lower risk of sample degradation. 70,71 The solvent systems for these analytical techniques commonly use chloroform or dichloromethane and aqueous buffer with pH gradients to purify alkaloids.…”

Section: Isolation Of Alkaloids From Extractsmentioning

confidence: 99%

Sustainable approaches for the study of alkaloids from plants using supercritical fluid-based processes

et al. 2022

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Section: Isolation Of Alkaloids From Extractsmentioning

confidence: 99%

Sustainable approaches for the study of alkaloids from plants using supercritical fluid-based processes

et al. 2022

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“…Additionally, it has the merits of simple pretreatment operation, high reproducibility, and high recovery rate [39]. The applications of this approach have been widely used in the separation and purification of a variety of active ingredients from natural products [40][41][42][43][44][45][46]. In our previous studies, HSCCC was successfully applied to separate and isolate polyphenols at a large scale from different plants [47][48][49].…”

Section: Introductionmentioning

confidence: 99%

Characterization, Large-Scale HSCCC Separation and Neuroprotective Effects of Polyphenols from Moringa oleifera Leaves

et al. 2022

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Moringa oleifera leaves have been widely used for the treatment of inflammation, diabetes, high blood pressure, and other diseases, due to being rich in polyphenols. The main objective of this work was to largely separate the main polyphenols from Moringa oleifera leaves using the technique of high-speed counter-current chromatography (HSCCC). The phenolic composition in Moringa oleifera leaves was first analyzed qualitatively and quantitatively by UPLC-Q-Exactive Orbitrap/MS and UPLC-QqQ/MS, respectively, indicating that quercetin and kaempferol derivatives, phenolic acid and apigenin are the main polyphenols in Moringa oleifera leaves, with quercetin and kaempferol derivatives predominating. Furthermore, the conditions of HSCCC for large-scale separation of polyphenols from Moringa oleifera leaves were optimized, which included the selection of the solvent system, flow rate and the sample load. Only by one-step HSCCC separation (within 120 min) under the optimized conditions, six quercetin and kaempferol derivatives, a phenolic acid and an apigenin could be individually isolated at a large scale (yield from 10% to 98%), each of which possessed high purity. Finally, the isolated polyphenols and phenolic extract from Moringa oleifera leaves (MLPE) were verified to have strong neuroprotective activities against H2O2-induced oxidative stress in PC-12 cells, suggesting that these compounds would contribute to the main beneficial effects of Moringa oleifera leaves.

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“…As the advantages mentioned above, LLC is usually positioned as the first dimension in a 2D separation system. Applications of 2D-LLC have been investigated on counter-current chromatography (CCC) and centrifugal partition chromatography (CPC) apparatus at a preparative scale [10][11][12][13]. In consideration of complementary action between CCC and LC, 2D CCC-LC with a large loading capacity and high resolution is a powerful tool for preparative separating pure targets from the complicated mixture and recovering valuable samples.…”

Section: Introductionmentioning

confidence: 99%

In silico screening of off‐line comprehensive two‐dimensional counter‐current chromatography with liquid chromatography for four saponins isolation

Sun

Zhang

Bao

et al. 2022

J of Separation Science

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Being restrained by the limited peak capacity, one‐dimensional chromatography usually leads to an unsatisfactory separation with low purity of compounds in a complex mixture. To obtain more highly pure targets for standard reference and to discover new substances for structural elucidation, two‐dimensional chromatography is more and more prevalent in many fields. As few metrics on assessment of the preparative capability of two‐dimensional chromatographic separations are reported, a methodology of in silico screening of various two‐dimensional chromatographic separations with a minimal number of experiments was demonstrated in this work, which was based on three descriptors including the occupation rate of peaks and system homogeneity of a two‐dimensional separation space, and the minimal distance of all nearest‐neighbor distances of peaks. Combining the advantages of counter‐current chromatography and liquid chromatography, we elaborated the methodology by employing off‐line comprehensive two‐dimensional counter‐current chromatography with liquid chromatography to be in silico screened for separation of four saponins from Panax notoginseng at an analytical scale to simulate the case of preparative scale transfer. The predictive results were presented by two‐dimensional contour plots and verified by experiments. The result showed that the experimental results were in general accord with the predictive results.

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Efficient separation of N-Alkylamides from Piper longum L. using off-line two-dimensional coupled with gradient high-speed counter-current chromatography

Cited by 4 publications

References 38 publications

Sustainable approaches for the study of alkaloids from plants using supercritical fluid-based processes

Sustainable approaches for the study of alkaloids from plants using supercritical fluid-based processes

Characterization, Large-Scale HSCCC Separation and Neuroprotective Effects of Polyphenols from Moringa oleifera Leaves

In silico screening of off‐line comprehensive two‐dimensional counter‐current chromatography with liquid chromatography for four saponins isolation

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