Characterization, Large-Scale HSCCC Separation and Neuroprotective Effects of Polyphenols from Moringa oleifera Leaves

Gao, Qian; Wei, Zongmin; Liu, Yun; Fang, Wang; Zhang, Shuting; Serrano, Carmo; Li, Lingxi; Sun, Baoshan

doi:10.3390/molecules27030678

Cited by 11 publications

(7 citation statements)

References 98 publications

(102 reference statements)

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“…Quercetin is among the abundant flavonoids of M. oleifera (Vergara-jimenez, Almatrafi, & Fernandez, 2017). The leaves contain the highest quercetin, among other flavonoid compounds with four of the quercetin derivatives made up the majority of the flavonoids (Coppin et al, 2013;Zhu et al, 2020;Gao et al, 2022). Moreover, Makita et al (2016) and Lin et al (2018) have revealed that the quercetin present is in significant amounts as glycosides associated with a variety of sugar moieties.…”

Section: Quercetinmentioning

confidence: 99%

“…The neuroprotective activities of astragalin are associated with the free radical scavenging ability and oxidative stress-induced influences on the brain neuronal cells ( Wasik and Antkiewicz-Michaluk, 2017 ; Riaz et al, 2018 ). Astragalin and isoquercitrin isolated from M. oleifera leaves showed potent anti-oxidative effects as enhanced cell viabilities were observed in H2O2-induced oxidative stress in PC-12 cells ( Gao et al, 2022 ). Although the associated active substances involved in the mechanism were not described, the study has found that the rate of survival for the damaged cells improved as the treatment concentration of isolated compounds increased, suggesting the effect of anti-oxidative activities.…”

Section: Neuroprotective and Anti-neuroinflammatory Effects Of ...mentioning

confidence: 99%

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An insight into the neuroprotective and anti-neuroinflammatory effects and mechanisms of Moringa oleifera

et al. 2023

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Neurodegenerative diseases (NDs) are sporadic maladies that affect patients’ lives with progressive neurological disabilities and reduced quality of life. Neuroinflammation and oxidative reaction are among the pivotal factors for neurodegenerative conditions, contributing to the progression of NDs, such as Parkinson’s disease (PD), Alzheimer’s disease (AD), multiple sclerosis (MS) and Huntington’s disease (HD). Management of NDs is still less than optimum due to its wide range of causative factors and influences, such as lifestyle, genetic variants, and environmental aspects. The neuroprotective and anti-neuroinflammatory activities of Moringa oleifera have been documented in numerous studies due to its richness of phytochemicals with antioxidant and anti-inflammatory properties. This review highlights up-to-date research findings on the anti-neuroinflammatory and neuroprotective effects of M. oleifera, including mechanisms against NDs. The information was gathered from databases, which include Scopus, Science Direct, Ovid-MEDLINE, Springer, and Elsevier. Neuroprotective effects of M. oleifera were mainly assessed by using the crude extracts in vitro and in vivo experiments. Isolated compounds from M. oleifera such as moringin, astragalin, and isoquercitrin, and identified compounds of M. oleifera such as phenolic acids and flavonoids (chlorogenic acid, gallic acid, ferulic acid, caffeic acid, kaempferol, quercetin, myricetin, (-)-epicatechin, and isoquercitrin) have been reported to have neuropharmacological activities. Therefore, these compounds may potentially contribute to the neuroprotective and anti-neuroinflammatory effects. More in-depth studies using in vivo animal models of neurological-related disorders and extensive preclinical investigations, such as pharmacokinetics, toxicity, and bioavailability studies are necessary before clinical trials can be carried out to develop M. oleifera constituents into neuroprotective agents.

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Section: Quercetinmentioning

confidence: 99%

Section: Neuroprotective and Anti-neuroinflammatory Effects Of ...mentioning

confidence: 99%

An insight into the neuroprotective and anti-neuroinflammatory effects and mechanisms of Moringa oleifera

et al. 2023

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“…Moringa can be used as a potent neuro-protectant by reducing the formation of ROS, thereby protecting the brain (Kirisattayakul et al, 2013). The isolated polyphenols and phenolic extract from moringa leaves have demonstrated strong neuroprotective activities against H2O2-induced oxidative stress in PC-12 cells (Gao et al, 2022). A cardioprotective alkaloid ( L-N,αrhamnopyranosyl vincosamide (VR), isolated from the leaves of markedly reduced isoproterenol (ISO)-M. oleifera, induced increase in the levels of serum cardiac markers (troponin-T, creatine kinase-MB, lactate dehydrogenase and glutamate pyruvate transaminase as well as cardiac lipid peroxidation) with a parallel increase in the antioxidants suggesting its cardio-protective and free radical scavenging potential (Panda et al, 2013).…”

Section: Neuro-protective and Cardio-protective Activitiesmentioning

confidence: 99%

Moringa oleifera Lam: A versatile medicinal tree in tropical and subtropical countries

Gupta¹

2022

Asian J Pharm Pharmacol

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Moringa oleifera Lam (Moringaceae), with several common names such as "sahajan", "drumstick", "super food", "miracle tree", "horseradish" and "ben oil tree", is distributed throughout in tropical and subtropical regions of the world. This plant is a panacea, since different parts of it have long been used in herbal medicines by Indians and Africans to cure more than 300 diseases. Moringa is one of the most nutrient-rich plants as it contains over 90 nutrients, 46 antioxidants, 18 amino acids, including 8 essential amino acids. The leaves, pods and seeds of it show presence of a variety of essential phytochemicals. With no known reports of side effects, different parts of the plant are used to treat malnutrition, diabetes, blindness, anemia, hypertension, stress, depression, skin, arthritis, joints, liver and kidney disorders. The powder from seeds and leaves is widely used in water and effluent treatment to improve water quality, which has special applicability in intensive animal production systems, such as aquaculture. The plant possesses many pharmacological attributes such as hypoglycemic, anti-diabetic anti-oxidant, hepato-protective, neuro-protective, cardio-protective, chemo-preventive, anti-cancer, anti-ulcer, cytotoxic, anti-inflammatory, anti-microbial, herbicidal, anti-hypertensive, enzyme inhibition, uterotonic, and fertility stimulator. Due to high nutritional value and several medicinal properties, the plant may act as a nutritional and medical alternative for socially neglected populations. This review will present an updated compilation of the published research on the medicinal characteristics, phytochemical composition, recent advances in pharmacognosy of moringa. and pharmacological properties along with

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“…One of its well-known benefits is that it does not require a solid support matrix for the stationary phase, allowing the preparative separation of solutes in a two-phase solvent system [ 28 ]. This feature eliminates the irreversible adsorption loss of the sample caused by the solid support matrix in traditional chromatography columns [ 29 ]. In addition, HSCCC offers several advantages, such as high recovery, high efficiency, simple pretreatment operation, high reproducibility, and ease of scaleup [ 27 – 29 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid Separation of Asiatic Acid, Quercetin, and Kaempferol from Traditional Chinese Medicine Centella asiatica (L.) Urban Using HSCCC-Semi-Prep-HPLC and the Assessment of Their Potential as Fatty Acid Synthase Inhibitors

Xia,

Li,

Liu

et al. 2023

International Journal of Analytical Chemistry

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The main objective of this study was to rapidly separate asiatic acid (AA), quercetin (QCN), and kaempferol (KPL) from Centella asiatica (L.) Urban using high-speed counter-current chromatography (HSCCC) in tandem with the UV detector of semipreparative high-performance liquid chromatography (Semi-Prep-HPLC) and to evaluate their potential as inhibitors of fatty acid synthetase (FAS). To efficiently prepare large amounts of AA, QCN, and KPL from Centella asiatica (L.) Urban, rapid and simple methods by HSCCC were established respectively based on the partition coefficients (K values) of crude samples. The conditions of HSCCC-Semi-Prep-HPLC for the large-scale separation of AA, QCN, and KPL from Centella asiatica (L.) Urban were established and optimized. This included selecting the solvent system, flow rate, rotation speed, and so on. HSCCC-Semi-Prep-HPLC was successfully applied to separate and purify AA, QCN, and KPL, with n-hexane-n-butanol-methanol-water (3 : 1 : 3 : 3, V : V : V : V) as the solvent system for AA, which was detected at a wavelength of 210 nm with the stationary phase retention of 70%, and with n-hexane-ethyl acetate-methanol-water (0.8 : 0.9 : 1.2 : 1, V : V : V : V) as the solvent system for the co-separation of QCN and KPL, which was detected at a wavelength of 254 nm with the stationary phase retention of 65%. AA could be isolated at a large scale with high purity (>91.0%) in only one-step HSCCC-Semi-Prep-HPLC separation (within 150 min) under the optimized conditions. Meanwhile, QCN and KPL could be simultaneously isolated at a large scale with high purity (>99.1%) by another one-step HSCCC-Semi-Prep-HPLC separation (within 240 min) under the optimized conditions. The assessment of inhibition potential revealed that AA exhibited the strongest inhibitory effect on FAS, with an IC50 of 9.52 ± 0.76 μg/mL. Madecassic acid (MA) followed closely with IC50 values of 10.84 ± 0.92 μg/mL. QCN and KPL showed similar and relatively weaker inhibitory effects on FAS, with IC50 values of 43.09 ± 2.98 μg/mL and 36.90 ± 1.83 μg/mL, respectively. Overall, the HSCCC-Semi-Prep-HPLC method proved to be a highly efficient and reliable technique for separating AA, QCN, and KPL from Centella asiatica (L.) Urban, and the isolated compounds showed potential as FAS inhibitors.

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Characterization, Large-Scale HSCCC Separation and Neuroprotective Effects of Polyphenols from Moringa oleifera Leaves

Cited by 11 publications

References 98 publications

An insight into the neuroprotective and anti-neuroinflammatory effects and mechanisms of Moringa oleifera

An insight into the neuroprotective and anti-neuroinflammatory effects and mechanisms of Moringa oleifera

Moringa oleifera Lam: A versatile medicinal tree in tropical and subtropical countries

Rapid Separation of Asiatic Acid, Quercetin, and Kaempferol from Traditional Chinese Medicine Centella asiatica (L.) Urban Using HSCCC-Semi-Prep-HPLC and the Assessment of Their Potential as Fatty Acid Synthase Inhibitors

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