Efficient recovery of potent tumour-infiltrating lymphocytes through quantitative immunomagnetic cell sorting

Future of Digital Assays to Resolve Clinical Heterogeneity of Single Extracellular Vesicles

“…Techniques that exploit physical energy fields to separate cells (magnetic, , acoustic, , and dielectrophoretic forces , ) are promising tools to manipulate nanoscale EVs. , They offer the versatility to handle many subpopulations of a complex sample and preserve the structural integrity of EVs, whereas classical isolation methods often alter the morphology, content, and functions of EVs. − …”

Advancing microfluidic diagnostic chips into clinical use: a review of current challenges and opportunities

“…3,4 From an engineering perspective, we are at an exciting stage marked by an acceleration in the capabilities of new μDx devices to detect, sort, and quantify rare and clinically useful biomarkers from complex samples. [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] The progress has been all the more astonishing given the relative youth of the field, the immense challenges posed by the complexity of biological systems, and the diverse and multitudinous background of material present in patient samples, such as blood, from which biomarkers must be measured. In the last few years, μDx technology has begun to reach the level of maturity for commercialization, regulatory approval, and practical clinical use (Table 1).…”

“…5,38 Further, the increased sensitivity of μDx decreases the volume of sample required per assay, allowing higher levels of multiplexing on a given volume of a patient sample. 18 Due to the potential for low-cost and minimally invasive measurements, μDx chips can also enable increased numbers of longitudinal measurements. 39 Droplet microfluidics techniques, such as single cell sequencing and digital PCR, have found particular utility in multiplexing the profiling of nucleic acids often down to the single-cell level.…”

Nanoparticle Amplification Labeling for High-Performance Magnetic Cell Sorting

“…Cell sorting, the process of separating cell phenotypes of interest from a heterogeneous population, is an enabling technology for modern biotechnology . The scale of cell sorting can easily reach hundreds of billions of cells during fundamental biological studies (i.e., genome-wide CRISPR screen) − or therapeutic applications (i.e., manufacturing of therapeutic cells). − To meet the demand for sorting such a large number of cells, it is critical to develop high-throughput and highly controllable cell-sorting processes.…”

“…MACS utilizes magnetic nanoparticles conjugated with antibodies to bind to the cells of interest and pull magnetically labeled populations up or down using an external magnetic field . Although MACS has high levels of throughput, extremely good scalability, and relatively low cost, the controllability over the sorting process remains low. , The commercial version of MACS is only capable of sorting cells into binary populations on the basis of a single, strongly expressed protein biomarker per run . To improve the controllability of magnetic-activated sorting, microfluidic technologies have been implemented to successfully achieve multimarker sorting, , quantitative sorting, − and sorting based on nonprotein targets .…”