Advancing microfluidic diagnostic chips into clinical use: a review of current challenges and opportunities

Iyer, Vasant; Yang, Zijian; Ko, Jina; Weissleder, Ralph; Issadore, David

doi:10.1039/d2lc00024e

Cited by 18 publications

(17 citation statements)

References 105 publications

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“…Besides optimization of the magnetic beads, antibodies, and procedures for the isolation of EVs from biofluids, advances in alternative EV isolation and analysis methods, such as microfluidic diagnostic devices, hold promise for future progress in this field. Numerous such devices and technologies have emerged in recent years allowing the detection of biomolecules on micro- or nanoscales [ 84 ]. Here we discuss only studies relevant to CNS-originating EVs and refer to the review by Iyer et al [ 84 ] for a more general overview of diagnostic microfluidic devices for EV and biomarker analysis.…”

Section: Advances In Ev Isolation Methodsmentioning

confidence: 99%

“…Numerous such devices and technologies have emerged in recent years allowing the detection of biomolecules on micro- or nanoscales [ 84 ]. Here we discuss only studies relevant to CNS-originating EVs and refer to the review by Iyer et al [ 84 ] for a more general overview of diagnostic microfluidic devices for EV and biomarker analysis.…”

Section: Advances In Ev Isolation Methodsmentioning

confidence: 99%

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Biomarkers for parkinsonian disorders in CNS-originating EVs: promise and challenges

Dutta¹,

Hornung

Taha

et al. 2023

Acta Neuropathol

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Extracellular vesicles (EVs), including exosomes, microvesicles, and oncosomes, are nano-sized particles enclosed by a lipid bilayer. EVs are released by virtually all eukaryotic cells and have been shown to contribute to intercellular communication by transporting proteins, lipids, and nucleic acids. In the context of neurodegenerative diseases, EVs may carry toxic, misfolded forms of amyloidogenic proteins and facilitate their spread to recipient cells in the central nervous system (CNS). CNS-originating EVs can cross the blood–brain barrier into the bloodstream and may be found in other body fluids, including saliva, tears, and urine. EVs originating in the CNS represent an attractive source of biomarkers for neurodegenerative diseases, because they contain cell- and cell state-specific biological materials. In recent years, multiple papers have reported the use of this strategy for identification and quantitation of biomarkers for neurodegenerative diseases, including Parkinson’s disease and atypical parkinsonian disorders. However, certain technical issues have yet to be standardized, such as the best surface markers for isolation of cell type-specific EVs and validating the cellular origin of the EVs. Here, we review recent research using CNS-originating EVs for biomarker studies, primarily in parkinsonian disorders, highlight technical challenges, and propose strategies for overcoming them.

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Section: Advances In Ev Isolation Methodsmentioning

confidence: 99%

Section: Advances In Ev Isolation Methodsmentioning

confidence: 99%

Biomarkers for parkinsonian disorders in CNS-originating EVs: promise and challenges

Dutta¹,

Hornung

Taha

et al. 2023

Acta Neuropathol

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“…Nevertheless, weakness of polymer materials includes thermal conductivity and low thermal resistance. The surface modification of polymers also needs to be further explored [ 74 , 75 ]. In addition, a practical solution for the processing and integration of the whole microfluidic system should be presented and optimized [ 74 ].…”

Section: Multiplex Nucleic Acid Sensors On Microfluidic Platformsmentioning

confidence: 99%

“…In addition, a practical solution for the processing and integration of the whole microfluidic system should be presented and optimized [ 74 ]. Finally, microfluidic devices need to be tested in large-scale clinical trials before being commercialized [ 75 ].…”

Section: Multiplex Nucleic Acid Sensors On Microfluidic Platformsmentioning

confidence: 99%

Multiplex Detection of Infectious Diseases on Microfluidic Platforms

Chen

et al. 2023

Biosensors

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Infectious diseases contribute significantly to the global disease burden. Sensitive and accurate screening methods are some of the most effective means of identifying sources of infection and controlling infectivity. Conventional detecting strategies such as quantitative polymerase chain reaction (qPCR), DNA sequencing, and mass spectrometry typically require bulky equipment and well-trained personnel. Therefore, mass screening of a large population using conventional strategies during pandemic periods often requires additional manpower, resources, and time, which cannot be guaranteed in resource-limited settings. Recently, emerging microfluidic technologies have shown the potential to replace conventional methods in performing point-of-care detection because they are automated, miniaturized, and integrated. By exploiting the spatial separation of detection sites, microfluidic platforms can enable the multiplex detection of infectious diseases to reduce the possibility of misdiagnosis and incomplete diagnosis of infectious diseases with similar symptoms. This review presents the recent advances in microfluidic platforms used for multiplex detection of infectious diseases, including microfluidic immunosensors and microfluidic nucleic acid sensors. As representative microfluidic platforms, lateral flow immunoassay (LFIA) platforms, polymer-based chips, paper-based devices, and droplet-based devices will be discussed in detail. In addition, the current challenges, commercialization, and prospects are proposed to promote the application of microfluidic platforms in infectious disease detection.

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“…A microfluidic device is a platform to realize this technology. Researchers have studied and applied microfluidic devices in medicine [ 1 , 2 , 3 , 4 ], food safety [ 5 ], and environmental monitoring [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Research Progress and Future Trends of Microfluidic Paper-Based Analytical Devices in In-Vitro Diagnosis

et al. 2022

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In vitro diagnosis (IVD) has become a hot topic in laboratory research and achievement transformation. However, due to the high cost, and time-consuming and complex operation of traditional technologies, some new technologies are being introduced into IVD, to solve the existing problems. As a result, IVD has begun to develop toward point-of-care testing (POCT), a subdivision field of IVD. The pandemic has made governments and health institutions realize the urgency of accelerating the development of POCT. Microfluidic paper-based analytical devices (μPADs), a low-cost, high-efficiency, and easy-to-operate detection platform, have played a significant role in advancing the development of IVD. μPADs are composed of paper as the core material, certain unique substances as reagents for processing the paper, and sensing devices, as auxiliary equipment. The published reviews on the same topic lack a comprehensive and systematic introduction to μPAD classification and research progress in IVD segmentation. In this paper, we first briefly introduce the origin of μPADs and their role in promoting IVD, in the introduction section. Then, processing and detection methods for μPADs are summarized, and the innovative achievements of μPADs in IVD are reviewed. Finally, we discuss and prospect the upgrade and improvement directions of μPADs, in terms of portability, sensitivity, and automation, to help researchers clarify the progress and overcome the difficulties in subsequent μPAD research.

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Advancing microfluidic diagnostic chips into clinical use: a review of current challenges and opportunities

Abstract: Microfluidic diagnostic (µDX) technologies miniaturize sensors and actuators to the length-scales that are relevant to biology: the micrometer scale to interact with cells and the nanometer scale to interrogate biology’s...

Cited by 18 publications

References 105 publications

Biomarkers for parkinsonian disorders in CNS-originating EVs: promise and challenges

Biomarkers for parkinsonian disorders in CNS-originating EVs: promise and challenges

Multiplex Detection of Infectious Diseases on Microfluidic Platforms

Research Progress and Future Trends of Microfluidic Paper-Based Analytical Devices in In-Vitro Diagnosis

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