Efficient production and recovery of diterpenoid tanshinones in Salvia miltiorrhiza hairy root cultures with in situ adsorption, elicitation and semi-continuous operation

Qing, Yan; Hu, Zongding; Tan, Ren Xiang; Wu, Jianyong

doi:10.1016/j.jbiotec.2005.04.020

Cited by 81 publications

(40 citation statements)

References 18 publications

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“…The above results indicate that in a hairy root culture the maximum of the biomass production coincides with the maximum of the diterpenoid accumulation. This behaviour has been detected for many other metabolites produced in in vitro cultures, for expample taxanes in a Taxus baccata cell culture (Navia-Osorio et al, 2002) or tanshinones in hairy roots of Salvia miltiorrhiza (Yan et al, 2005). In the case of S. sclarea transformed roots, both parameters, biomass and diterpenoid content, achieved the highest values on 30 day of the culture.…”

Section: ð1mentioning

confidence: 67%

Diterpenoid Production in Hairy Root Culture of Salvia sclarea L.

Kuźma

Bruchajze

Wysokińska

2008

Zeitschrift Für Naturforschung C

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Growth and diterpenoid accumulation (salvipisone, ferruginol, aethiopinone and 1-oxoaethiopinone) during the growth cycle of a Salvia sclarea hairy root culture are described. The roots transformed by Agrobacterium rhizogenes (LBA 9402) were cultured in half-strength B5 liquid medium supplemented with 30 g L Ð1 sucrose under light (16 h/8 h light/dark). A culture period of 30 days was optimal for both biomass and diterpenoid production. The total content of four diterpenoids in the hairy roots [(27.3 ð 0.6) mg g Ð1 dry weight] was higher than that of roots of field-grown S. sclarea plants [(3.15 ð 0.15) mg g Ð1 dry weight]. In transformed roots, aethiopinone was the main diterpenoid, whereas the principal diterpenoid of natural roots was salvipisone.

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Section: ð1mentioning

confidence: 67%

Diterpenoid Production in Hairy Root Culture of Salvia sclarea L.

Kuźma

Bruchajze

Wysokińska

2008

Zeitschrift Für Naturforschung C

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“…Adsorbent preparation X-5 macroporous polystyrene resin (Nankai University Chemical Plant, Tianjin, China) was used as adsorbent and pre-treated as previously described (Yan et al 2005).…”

Section: Elicitor Preparationmentioning

confidence: 99%

“…The method and instruments for extracting tanshinones from the roots, the resin, and the culture medium were described previously (Yan et al 2005).…”

Section: Extraction and Analysis Of Tanshinonesmentioning

confidence: 99%

“…The combination of resins and elicitors is an effective technology for promoting the synthesis and recovery of target product in plant tissue cultures (Buitelaar et al 1993;Asada and Shuler 1989). Our previous study showed that in situ adsorption (using a hydrophobic polymeric resin, X-5) combined with elicitation (using a material derived from yeast extract) enhanced diterpenoid tanshinone production in Salvia miltiorrhiza hairy root cultures (Yan et al 2005). However, the understanding of the mechanism of in situ adsorption and its combination with elicitation is limited and thus requires further investigation.…”

Section: Introductionmentioning

confidence: 99%

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Modeling of tanshinone synthesis and phase distribution under the combined effect of elicitation and in situ adsorption in Salvia miltiorrhiza hairy root cultures

Qing

Liu

2010

Biotechnol Lett

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Modeling of tanshinone synthesis and phase distribution under the combined effect of elicitation and adsorption was studied. The simulated results showed that enhancement of tanshinone production was mainly due to the effect of the elicitor and that resin addition resulted in adsorbance of the tanshinones from the root and alteration of tanshinone distribution. Furthermore, parameter sensitivities analysis showed that the rate of transport of tanshinones from the root to the medium was the important factor that influenced tanshinone accumulation in the resin. In conclusion, the modeling of tanshinone synthesis and phase distribution identified the process mechanism under the combined effect of elicitation and adsorption and this modeling can be used in similar plant tissue culture systems.

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“…This is the case for many crop cereals and other economically important grasses like vetiver. The addition of a liquid or solid phase to culture media has proven to be effective in maintaining cell viability over a long period of culture, with the advantage of enhancing the production and release of plant secondary metabolites from cells (Strobel et al 1991;Yan et al 2005) and physiological rationale for this has never been elucidated.…”

Section: Introductionmentioning

confidence: 99%

Sugar metabolism ofVetiveria zizanioidescells cultured with Amberlite XAD-4. Uptake of radiolabelled sugars, cell wall invertase activity and gene expression

Mucciarelli¹,

Bertea

Camusso³

2009

Journal of Plant Interactions

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Activated and non-activated Amberlite XAD-4 was added to the cell cultures of vetiver (Vetiveria zizanioides Stapf.) in order to study its effects as abiotic growth promoter. Amberlite XAD-4 increased vetiver cell viability, especially when used in the activated form. Results from labelling experiments in cell suspensions showed that XAD-4 prompted a significant decrease of sucrose Km, accompanied by an increased Vmax. Glucose uptake from the medium was almost doubled, as demonstrated by the increase of the radiolabelled fraction (25-fold the controls) in vetiver cells cultured with XAD-4. Affinity for fructose uptake by cells was not altered by the use of activated XAD-4, even though its uptake and Vmax increased. These results were further supported by a significant 7.5-fold increase in cell wall invertases (CWI) activity in XAD-4 elicited cells. Vetiver CWI gene expression was characterized by transcript RT-PCR analysis. The identity of the transcripts was confirmed by sequencing analysis. These results indicated the use of XAD-4 as an effective tool for vetiver cell biomass optimization.

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Efficient production and recovery of diterpenoid tanshinones in Salvia miltiorrhiza hairy root cultures with in situ adsorption, elicitation and semi-continuous operation

Cited by 81 publications

References 18 publications

Diterpenoid Production in Hairy Root Culture of Salvia sclarea L.

Diterpenoid Production in Hairy Root Culture of Salvia sclarea L.

Modeling of tanshinone synthesis and phase distribution under the combined effect of elicitation and in situ adsorption in Salvia miltiorrhiza hairy root cultures

Sugar metabolism ofVetiveria zizanioidescells cultured with Amberlite XAD-4. Uptake of radiolabelled sugars, cell wall invertase activity and gene expression

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