Modeling of tanshinone synthesis and phase distribution under the combined effect of elicitation and in situ adsorption in Salvia miltiorrhiza hairy root cultures

Qing, Yan; Wu, Jianyong; Liu, Rui

doi:10.1007/s10529-010-0484-5

Cited by 15 publications

(8 citation statements)

References 8 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Modeling of tanshinone synthesis and phase distribution under the combined effect of elicitation and in situ adsorption in HRCs of Salvia miltiorrhiza was studied by Yan et al [95]. The simulated results showed that the enhancement of tanshinone production was mainly due to the effect of the elicitor and that resin addition resulted in adsorbance of the tanshinones from the root and alteration of tanshinones distribution [95]. The rate of transport of tanshinones from the root to the medium was an important factor that influenced tanshinones accumulation in the resin [95].…”

Section: Elicitation and In Situ Product Removalmentioning

confidence: 99%

“…Elicitation of hairy roots leads to increased productivity of SM and helps in designing metabolic traps to allow adsorption of the product that makes product recovery easier, preventing feedback inhibition of accumulated synthesized metabolites, mitigating toxicity caused by waste materials released from cells, and preventing their degradation in the culture media . Permeabilization and in situ product adsorption result in many folds increase in product yield .…”

Section: Elicitors and Their Effects On Hrcsmentioning

confidence: 99%

“…Permeabilization and in situ product adsorption result in many folds increase in product yield . The introduction of an in situ product removal mechanism, such as a solid adsorbent (form solid–liquid systems) or an extraction solvent (liquid–liquid systems), to the culture medium can often effectively induce product release from plant cells and increase productivity . Nonionic polymeric ion‐exchange resins of Amberlite XAD series (Rohm and Haas, Philadelphia, PA) and macroporous polystyrene resin are commonly used as solid adsorbent.…”

Section: Elicitors and Their Effects On Hrcsmentioning

confidence: 99%

“…The simulated results showed that the enhancement of tanshinone production was mainly due to the effect of the elicitor and that resin addition resulted in adsorbance of the tanshinones from the root and alteration of tanshinones distribution . The rate of transport of tanshinones from the root to the medium was an important factor that influenced tanshinones accumulation in the resin . This modeling can be used in similar plant tissue culture systems in future.…”

Section: Elicitors and Their Effects On Hrcsmentioning

confidence: 99%

See 3 more Smart Citations

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

Halder

Sarkar

Jha

2019

Engineering in Life Sciences

232

134

View full text Add to dashboard Cite

Elicitation is a possible aid to overcome various difficulties associated with the large‐scale production of most commercially important bioactive secondary metabolites from wild and cultivated plants, undifferentiated or differentiated cultures. Secondary metabolite accumulation in vitro or their efflux in culture medium has been elicited in the undifferentiated or differentiated tissue cultures of several plant species by the application of a low concentration of biotic and abiotic elicitors in the last three decades. Hairy root cultures are preferred for the application of elicitation due to their genetic and biosynthetic stability, high growth rate in growth regulator‐free media, and production consistence in response to elicitor treatment. Elicitors act as signal, recognized by elicitor‐specific receptors on the plant cell membrane and stimulate defense responses during elicitation resulting in increased synthesis and accumulation of secondary metabolites. Optimization of various parameters, such as elicitor type, concentration, duration of exposure, and treatment schedule is essential for the effectiveness of the elicitation strategies. Combined application of different elicitors, integration of precursor feeding, or replenishment of medium or in situ product recovery from the roots/liquid medium with the elicitor treatment have showed improved accumulation of secondary metabolites due to their synergistic effect. This is a comprehensive review about the progress in the elicitation approach to hairy root cultures from 2010 to 2019 and the information provided is valuable and will be of interest for scientists working in this area of plant biotechnology.

show abstract

Section: Elicitation and In Situ Product Removalmentioning

confidence: 99%

Section: Elicitors and Their Effects On Hrcsmentioning

confidence: 99%

Section: Elicitors and Their Effects On Hrcsmentioning

confidence: 99%

Section: Elicitors and Their Effects On Hrcsmentioning

confidence: 99%

See 2 more Smart Citations

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

Halder

Sarkar

Jha

2019

Engineering in Life Sciences

232

134

View full text Add to dashboard Cite

show abstract

“…Another model for C. roseus was invented by Leduc et al () containing a detailed analysis on metabolic regulation and cell nutrition. Yan et al () focused on increased tanshinone yields in Salvia miltiorrhiza (Chinese sage) HR through elicitation and adsorption together with a corresponding model. Finally, Lenk et al () published an article concerning 3D growth measurement and modeling of Beta vulgaris (beet) HR in Petri dishes with solid medium.…”

Section: Other Models Worth Mentioningmentioning

confidence: 99%

Modeling of plant in vitro cultures: Overview and estimation of biotechnological processes

Maschke

Geipel

Bley

2014

Biotech & Bioengineering

View full text Add to dashboard Cite

Plant cell and tissue cultivations are of growing interest for the production of structurally complex and expensive plant-derived products, especially in pharmaceutical production. Problems with up-scaling, low yields, and high-priced process conditions result in an increased demand for models to provide comprehension, simulation, and optimization of production processes. In the last 25 years, many models have evolved in plant biotechnology; the majority of them are specialized models for a few selected products or nutritional conditions. In this article we review, delineate, and discuss the concepts and characteristics of the most commonly used models. Therefore, the authors focus on models for plant suspension and submerged hairy root cultures. The article includes a short overview of modeling and mathematics and integrated parameters, as well as the application scope for each model. The review is meant to help researchers better understand and utilize the numerous models published for plant cultures, and to select the most suitable model for their purposes.

show abstract

Ramified Challenges: Monitoring and Modeling of Hairy Root Growth in Bioprocesses—A Review

Lenk

Bley

2015

Advances in Biochemical Engineering/Biotechnology

View full text Add to dashboard Cite

The review presents a comprehensive overview on available solutions for the monitoring and modeling of various aspects of hairy root growth processes. Several online and offline measurement principles get explained exemplary and are being compared. It was found that no direct online measurement principle for hairy root biomass in submerged and solid-state culturing environment is available. However, certain indirect methods involving one or more measurement values have been developed for biomonitoring of hairy roots especially in bioreactors. In the field of modeling of hairy root growth processes, four independent architectures (continuous models, metabolic flux analysis, agent-based models, and artificial neural networks) are described and compared including literature references. The discussion is structured into microscopic model approaches, addressing only certain aspects of growth, and macroscopic model approaches, describing the hairy root network as a whole. An agent-based macroscopic model based on phenomenological data acquired with systematic imaging of hairy roots on culture dishes together with a 3D visualization of simulation results is presented in detail.

show abstract

Modeling of tanshinone synthesis and phase distribution under the combined effect of elicitation and in situ adsorption in Salvia miltiorrhiza hairy root cultures

Cited by 15 publications

References 8 publications

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures

Modeling of plant in vitro cultures: Overview and estimation of biotechnological processes

Ramified Challenges: Monitoring and Modeling of Hairy Root Growth in Bioprocesses—A Review

Contact Info

Product

Resources

About