Diterpenoid Production in Hairy Root Culture of Salvia sclarea L.

Kuźma, Łukasz; Bruchajze, Elzbieta; Wysokińska, Halina

doi:10.1515/znc-2008-7-827

Cited by 22 publications

(8 citation statements)

References 4 publications

(10 reference statements)

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“…In our study, after 42 days of the scale-up process, the hairy root showed a dark brown color in the center, due to viability loss, and clear and vital roots that had begun to turn brown externally. This behavior could be related to the decrease in nutritive compounds in the medium that bring to ageing and/or cell death; this is also observed by Kuzma et al [ 53 ] for the hairy roots of S. sclarea after 30 days of culture. Media composition could have a significant impact on hairy root growth in culture systems [ 54 ].…”

Section: Discussionsupporting

confidence: 60%

Abietane Diterpenoids from the Hairy Roots of Salvia corrugata

et al. 2021

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Salvia corrugata Vahl. is an interesting source of abietane and abeo-abietane compounds that showed antibacterial, antitumor, and cytotoxic activities. The aim of the study was to obtain transformed roots of S. corrugata and to evaluate the production of terpenoids in comparison with in vivo root production. Hairy roots were initiated from leaf explants by infection with ATCC 15834 Agrobacterium rhizogenes onto hormone-free Murashige and Skoog (MS) solid medium. Transformation was confirmed by polymerase chain reaction analysis of rolC and virC1 genes. The biomass production was obtained in hormone-free liquid MS medium using Temporary Immersion System bioreactor RITA®. The chromatographic separation of the methanolic extract of the untransformed roots afforded horminone, ferruginol, 7-O-acetylhorminone and 7-O-methylhorminone. Agastol and ferruginol were isolated and quantified from the hairy roots. The amount of these metabolites indicated that the hairy roots of S. corrugata can be considered a source of these compounds.

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Section: Discussionsupporting

confidence: 60%

Abietane Diterpenoids from the Hairy Roots of Salvia corrugata

et al. 2021

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“…During these studies, triterpenoids were also isolated. Oleanolic and ursolic acids were obtained from S. amplexicaulis, 114 as well as in vitro cultured shoots of SalVia nemorosa, 115 while maslinic acid, 3-epi-maslinic acid, tormentic acid, and 2R,3R,23trihydroxyolean-12-en-28-oic acid were isolated from tissue cultures of S. broussonetii Benth. 67…”

Section: Biological Activities Of Triterpenoids Found In Salwia Speciesmentioning

confidence: 99%

Bioactive Triterpenoids from Salvia Species

2006

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Salvia species are important medicinal and culinary plants, and they have been the subject of numerous chemical and biological studies. The bioactive triterpenoids of Salvia species, reported in the literature to date, are reviewed. About 200 triterpenoids, almost 80 of which are new, isolated, and characterized from about 100 Salvia species, are presented herein. In addition to the diverse biological activities of the pure triterpenoids, studies on biological activity of extracts of Salvia species are also described.

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“…We have also demonstrated that aethiopinone have an anti-proliferative activity in human melanoma cells, together with a safety profile for non-malignant cells 3 . Very low amount of these class pf diterpenes are synthesized in organs and tissues of several Salvia species and different biotechnological approaches (cell culture, hairy roots, elicitation) have been applied to enhance their content 3 , 5 , 30 . In the last few years, metabolic engineering strategies have been also attempted to increase the production of plant bioactive secondary metabolites, by tackling either single key-limiting genes or regulatory transcription factors, providing compelling evidence that it is feasible to boost the secondary metabolism in crop and medicinal plants 31 – 33 .…”

Section: Discussionmentioning

confidence: 99%

Coactivation of MEP-biosynthetic genes and accumulation of abietane diterpenes in Salvia sclarea by heterologous expression of WRKY and MYC2 transcription factors

Alfieri

Vaccaro

Cappetta

et al. 2018

Sci Rep

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Plant abietane diterpenoids (e.g. aethiopinone, 1- oxoaethiopinone, salvipisone and ferruginol), synthesized in the roots of several Salvia spp, have antibacterial, antifungal, sedative and anti-proliferative properties. Recently we have reported that content of these compounds in S. sclarea hairy roots is strongly depending on transcriptional regulation of genes belonging to the plastidial MEP-dependent terpenoid pathway, from which they mostly derive. To boost the synthesis of this interesting class of compounds, heterologous AtWRKY18, AtWRKY40, and AtMYC2 TFs were overexpressed in S. sclarea hairy roots and proved to regulate in a coordinated manner the expression of several genes encoding enzymes of the MEP-dependent pathway, especially DXS, DXR, GGPPS and CPPS. The content of total abietane diterpenes was enhanced in all overexpressing lines, although in a variable manner due to a negative pleiotropic effect on HR growth. Interestingly, in the best performing HR lines overexpressing the AtWRKY40 TF induced a significant 4-fold increase in the final yield of aethiopinone, for which we have reported an interesting anti-proliferative activity against resistant melanoma cells. The present results are also informative and instrumental to enhance the synthesis of abietane diterpenes derived from the plastidial MEP-derived terpenoid pathway in other Salvia species.

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Diterpenoid Production in Hairy Root Culture of Salvia sclarea L.

Cited by 22 publications

References 4 publications

Abietane Diterpenoids from the Hairy Roots of Salvia corrugata

Abietane Diterpenoids from the Hairy Roots of Salvia corrugata

Bioactive Triterpenoids from Salvia Species

Coactivation of MEP-biosynthetic genes and accumulation of abietane diterpenes in Salvia sclarea by heterologous expression of WRKY and MYC2 transcription factors

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