Efficient plant regeneration of Mesembryanthemum crystallinum via somatic embryogenesis

Effect of Urea-Type Cytokinins On The Adventitious Shoots Regeneration From Cotyledonary Node Explant in Thecommon Ice Plant,Mesembryanthemum Crystallinum

“…The frequency of shoot induction in the present study was greater than in other studies, in which t h e s h o o t s w e r e d e v e l o p e d t h r o u g h s o m a t i c embryogenesis (Abou-Mandour, 1992;Wang and Lü ttge, 1994;Cushman et al, 2000) and organogenesis (Meiners et al, 1991) in the ice plant. showed an improved procedure for the embryogenesis of the ice plant, but only 0.5-1.0% of the explants produced embryogenic calli.…”

“…In our procedure, regenerated plants were obtained within four months, which was more rapid than embryogenesis via a callus system described in elsewhere (Wang and Lüttge, 1994;Cushman et al, 2000). The first adventitious shoot formation from cotyledonary node explants was observed within 3-5 days and another shoots began to form quickly.…”

“…Hypocotyl explants have previously been used to induce shoots via somatic embryogenesis of ice plant (Wang and Lüttge, 1994;Cushman et al, 2000), and we first examined the effects of urea-type cytokinins on somatic embryogenesis in hypocotyl explants ( Table 1). Callus was produced in the medium, but embryos were not.…”

Highly efficient Agrobacterium-based transformation system for callus cells of the C3 halophyte Suaeda salsa

“…Accordingly, genetic transformation of halophytes should be a powerful technique to elucidate the mechanism(s) of salinity tolerance in these species. Genetic transformation systems have been reported for two halophytes: one of them being Mesembryanthemum crystallinum (Ishimaru 1999;Cushman et al 2000), a desert plant with a salt stress-induced shift from C 3 photosynthesis to crassulacean acid metabolism (CAM) and the development saltstoring bladder cells on shoots and leaves. Many genes have been cloned and characterized in the species (Meyer et al 1990), and M. crystallinum has often served as a model halophyte.…”

Pathways of ROS homeostasis regulation in Mesembryanthemum crystallinum L. calli exhibiting differences in rhizogenesis

“…M. crystallinum can be cultivated in vitro (Meiners et al 1991;Wang and Lüttge 1994;Yen et al 1997;Cushman et al 2000;, which allows to control the induction of regeneration in the cultured explants of this plant (Libik et al 2005) and allows to process plant morphogenesis by manipulating the level of some signaling molecules, such as ROS.…”