Pathways of ROS homeostasis regulation in Mesembryanthemum crystallinum L. calli exhibiting differences in rhizogenesis

Libik-Konieczny, Marta; Konieczny, Robert; Surówka, Ewa; Ślesak, Ireneusz; Michalec, Żaneta; Rozpądek, Piotr; Miszalski, Zbigniew

doi:10.1007/s11240-012-0136-7

Cited by 11 publications

(6 citation statements)

References 52 publications

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“…Some of the elementary processes and essential genes involved in this composite phenomenon have been identified (Sugiyama 1999 ; Philips 2004 ; Su et al 2007 ), but the regulation of their transcriptional activities underlying the processes of cell regeneration is poorly understood. The regulatory function of ROS and the antioxidant system in morphogenesis in vitro has been studied extensively (Siminis et al 1993 ; de Marco and Roubelakis-Angelakis 1996 ; Kairong et al 1999 ; Papadakis and Roubelakis-Angelakis 2002 ; Libik et al 2005 ; Li et al 2007 , 2009a , b ; Baťková et al 2008 ; Konieczny et al 2008 ; Gupta 2010 ; Vatankham et al 2010 ; Agrawall and Purohit 2012 ; Libik-Konieczny et al 2012 ). In vitro culture systems are good models for studying the physiological events in organogenesis since they make it easy to harvest a large amount of synchronized culture samples showing the state of organogenesis.…”

Section: Introductionmentioning

confidence: 99%

The localization of NADPH oxidase and reactive oxygen species in in vitro-cultured Mesembryanthemum crystallinum L. hypocotyls discloses their differing roles in rhizogenesis

Libik-Konieczny

Kozieradzka-Kiszkurno

Desel

et al. 2014

Protoplasma

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This work demonstrated how reactive oxygen species (ROS) are involved in the regulation of rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured on a medium containing 1-naphthaleneacetic acid (NAA). The increase of NADPH oxidase activity was correlated with an increase of hydrogen peroxide (H2O2) content and induction of mitotic activity in vascular cylinder cells, leading to root formation from cultured hypocotyls. Diphenylene iodonium (DPI), an inhibitor of NADPH oxidase, inhibited H2O2 production and blocked rhizogenesis. Ultrastructural studies revealed differences in H2O2 localization between the vascular cylinder cells and cortex parenchyma cells of cultured explants. We suggest that NADPH oxidase is responsible for H2O2 level regulation in vascular cylinder cells, while peroxidase (POD) participates in H2O2 level regulation in cortex cells. Blue formazan (NBT) precipitates indicating superoxide radical (O2•−) accumulation were localized within the vascular cylinder cells during the early stages of rhizogenesis and at the tip of root primordia, as well as in the distal and middle parts of newly formed organs. 3,3′-diaminobenzidine (DAB) staining of H2O2 was more intense in vascular bundle cells and in cortex cells. In newly formed roots, H2O2 was localized in vascular tissue. Adding DPI to the medium led to a decrease in the intensity of NBT and DAB staining in cultured explants. Accumulation of O2•− was then limited to epidermis cells, while H2O2 was accumulated only in vascular tissue. These results indicate that O2•− is engaged in processes of rhizogenesis induction involving division of competent cells, while H2O2 is engaged in developmental processes mainly involving cell growth.

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Section: Introductionmentioning

confidence: 99%

The localization of NADPH oxidase and reactive oxygen species in in vitro-cultured Mesembryanthemum crystallinum L. hypocotyls discloses their differing roles in rhizogenesis

Libik-Konieczny

Kozieradzka-Kiszkurno

Desel

et al. 2014

Protoplasma

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“…Mesembryanthemum crystallinum L. (ice plant) turned out to be a useful model for studying organogenesis and the role of oxidative stress in morphogenesis in vitro (Libik et al 2005a, b;Libik-Konieczny et al 2012;Konieczny et al 2014). Different SODs and CAT activities, as well as the corresponding changes in the content of H 2 O 2 in calluses exhibiting rhizogenic or embryogenic potential were suggested to be crucial in the induction of specific developmental pathway.…”

Section: Introductionmentioning

confidence: 99%

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Libik-Konieczny

Kozieradzka-Kiszkurno

Michalec-Warzecha

et al. 2017

Acta Physiol Plant

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The enrichment with antioxidants (glutathione or ascorbate) or prooxidants (alloxan, methylviologen, hydrogen peroxide) of root inducing medium significantly decreased rhizogenesis frequency (alloxan, hydrogen peroxide) or inhibited roots regeneration (ascorbate, methylviologen) during the in vitro culture of Mesembryanthemum crystallinum L. hypocotyls. The adventitious roots morphology, root hairs length and density, was also influenced. Changes in the rhizogenesis course were related to the differences in hydrogen peroxide concentration during following days of culture between explants exhibiting morphogenic potential and those without the ability to form adventitious roots. In explants with morphogenic potential, rhizogenesis induction was always accompanied by a high level of hydrogen peroxide followed by the decrease in H 2 O 2 content in following days. In contrast, in the explants without regeneration potential, the level of hydrogen peroxide was increasing during the culture period. Activity patterns of superoxide dismutase (SOD) and guaiacol peroxidase (POX) in the following days of culture were similar in the explants exhibiting regeneration potential cultured on different media. Total activity of SOD decreased during initial days of culture and then increased due to the activation of additional SOD isoform described as MnSODII. The activity of POX was low during the rhizogenesis induction, and then increased during following days of culture; the increase was correlated with the decrease in hydrogen peroxide content. In the explants without the ability to regenerate roots, the total activity of SOD was low throughout the whole culture period, whereas the POX activity was significantly higher than in hypocotyls with regeneration potential. It might be concluded that the increase in hydrogen peroxide during initial stages of rhizogenesis and the induction of MnSODII are prerequisites for adventitious roots formation from hypocotyls of M. crystallinum, independently in the presence of anti-or prooxidant in the culture medium.

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“…[9][10][11][12][13][14] The role of oxidative stress in morphogenesis in vitro has been examined in common ice plant (Mesembryanthemum crystallinum L.). 15 Calli differing in regeneration potential (i.e., rhizogenic or embryogenic) also differed in H 2 O 2 content as well as in SODs and CAT activities, suggesting the possible involvement of ROS in induction of different morphogenic pathways. 16 Oxygen constitutes molecules called Reactive Oxygen Species (ROS) that can react with other molecules during living functions of living aerobic cells and change the structures of the molecules they enter into the reaction.…”

Section: Introductionmentioning

confidence: 99%

Evaluation ofin vitrogenotoxic effects induced byin vitroanther culture conditions in sunflower

Çakmak

Uncuoğlu

Aydın

2019

Plant Signaling & Behavior

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Sunflower is a globally important oilseed, food, and ornamental crop. This study seeks to investigate the genotoxic effects of tissue culture parameters in sunflower calli tissues belongs to two genotypes obtained via anther culture. Anthers were pretreated with cold for 24 hours at 4°C and heat for 2 days at 35°C in the dark and plated onto media supplemented with different concentrations and combinations of 6-benzylaminopurine, 2,4-dichlorophenoxyacetic acid, α-naphthalene acetic acid and indole-3-acetic acid. Obtaining calli tissues were used to detect the DNA damage levels by Comet assay, evaluating changes on superoxide dismutase and guaiacol peroxidase activities derived from in vitro culture factors. 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 2 mg/L α-naphthalene acetic acid from plant growth regulators showed acute genotoxic effect while 0.5 mg/L indole-3-acetic acid and 0.5 mg/L α-naphthalene acetic acid showed no genotoxic effect. Total protein content analysis of antioxidant enzymes revealed that although superoxide dismutase activity did not increase, Guaiacol peroxidase (GPOX) activity decreased in comparison to control. The obtained results have indicated that in vitro culture factors apparently lead to genotoxicity and oxidative stress.

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Pathways of ROS homeostasis regulation in Mesembryanthemum crystallinum L. calli exhibiting differences in rhizogenesis

Cited by 11 publications

References 52 publications

The localization of NADPH oxidase and reactive oxygen species in in vitro-cultured Mesembryanthemum crystallinum L. hypocotyls discloses their differing roles in rhizogenesis

The localization of NADPH oxidase and reactive oxygen species in in vitro-cultured Mesembryanthemum crystallinum L. hypocotyls discloses their differing roles in rhizogenesis

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Evaluation ofin vitrogenotoxic effects induced byin vitroanther culture conditions in sunflower

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