Sunflower is a globally important oilseed, food, and ornamental crop. This study seeks to investigate the genotoxic effects of tissue culture parameters in sunflower calli tissues belongs to two genotypes obtained via anther culture. Anthers were pretreated with cold for 24 hours at 4°C and heat for 2 days at 35°C in the dark and plated onto media supplemented with different concentrations and combinations of 6-benzylaminopurine, 2,4-dichlorophenoxyacetic acid, α-naphthalene acetic acid and indole-3-acetic acid. Obtaining calli tissues were used to detect the DNA damage levels by Comet assay, evaluating changes on superoxide dismutase and guaiacol peroxidase activities derived from in vitro culture factors. 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 2 mg/L α-naphthalene acetic acid from plant growth regulators showed acute genotoxic effect while 0.5 mg/L indole-3-acetic acid and 0.5 mg/L α-naphthalene acetic acid showed no genotoxic effect. Total protein content analysis of antioxidant enzymes revealed that although superoxide dismutase activity did not increase, Guaiacol peroxidase (GPOX) activity decreased in comparison to control. The obtained results have indicated that in vitro culture factors apparently lead to genotoxicity and oxidative stress.
INTRODUCTION: Patau syndrome was the first described by Patau et al. and associated with trisomy 13. The syndrome is characterized by microphthalmia, polydactyly, and cleft lip-palate. In this case report, we discuss the anesthesiology approach and management in a 2-year-old girl with Patau syndrome undergoing bilateral vitrectomy surgery. CASE REPORT: A 2-year-old girl weighing 12.5 kg was undergoing bilateral vitrectomy surgery. There was no additional pathology related to the cardiovascular, central nervous, and respiratory systems. Following anesthesia induction, the patient was intubated using a # 4.5 uncuffed endotracheal tube. At the end of the operation, 3 mg/kg sugammadex was given to the patient and she was transferred to the remission unit without any problems. During her follow-up in the unit, nurses called for help after her oxygen saturation level dropped to 90%. Mask ventilation support was supplied with a chin lift maneuver. As re-curarization was thought to be associated with the situation, an additional sugammadex dose was given to the patient. After 20 minutes of follow up, she was transferred to the pediatric ward in a steady state. CONCLUSION: The doses of anesthetic and analgesic drugs should be titrated carefully because intraoperative opioid and neuromuscular blocker use can lead to postoperative respiratory problems. Therefore, close follow-up of patients is essential in the postoperative period, as in the intraoperative period.
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