Effect of Urea-Type Cytokinins On The Adventitious Shoots Regeneration From Cotyledonary Node Explant in Thecommon Ice Plant,Mesembryanthemum Crystallinum

Sunagawa, Haruki; Agarie, Sakae; Umemoto, Makiko; Makishi, Yuko; Nose, Akihiro

doi:10.1626/pps.10.47

Cited by 18 publications

(16 citation statements)

References 35 publications

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“…Dinçer achieved on a MS medium containing TDZ and IBA. Our present finding is also not supported with other reports demonstrating that the urea-type cytokinins, TDZ and CPPU was more effective for the induction and the morphogenesis of adventitious multiple shoots from cotyledonary node explants of M. crystallinum plants than BAP [29].…”

Section: Shoot Induction and Multiplication Via Micropropagationcontrasting

confidence: 99%

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (Carthamus tinctorius L.) cv. NARI-H-15

Vijayakumar¹,

Ponmanickam²,

Samuel³

et al. 2017

AJPS

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The effect of meta-Topolin (mT) was assessed to develop a reliable protocol for efficient plant regeneration of safflower (Carthamus tinctorius L.) cv.NARI-H-15. For micropropagation, 7 -9 days old shoot-tip explants cultured on MS basal medium supplemented with 3.0 mg/L meta-Topolin (mT) + 0.5 mg/L CPPU showed 97.7% adventitious shoot formation (42.4 shootlets) than node after 45 days of culture. For organogenesis, the seedling explants of immature leaf cultured on 1.5 mg/L CPPU or 1.5 mg/L NAA fortified medium produced high amount of callus than cotyledon and stem calli after 60 days of culture. However, MS basal medium fortified with 4.0 mg/L mT + 1.5 mg/L CPPU was found beneficial to stimulate 100% organogenic response (74.7 shootlets) from immature leaf calli than cotyledon and stem derived calli after 45 days of culture. The healthy plantlets obtained from micropropagation and organogenesis process cultured on 1/4 MS basal salts, 1.5% sucrose (w/v) and 0.8% agar (w/v) medium supplemented with NAA (1.5 mg/L) and mT (0.1 mg/L) produced maximum of 96% (12.8 rootlets) and 84% (7.3 rootlets) adventitious rooting, respectively than mT and CPPU tested medium. However, maximum of 67% and 42% survival rate was noticed when in vitro raised plants from micropropagation and organogenesis were hardened in pots containing soil mix and maintained under green house condition. This optimized regeneration protocol might be helpful in regeneration of new genotypes and cultivars of safflower to improve agronomic traits through in vitro selection process and Agrobacterium-mediated genetic transformation system. Keywords

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Section: Shoot Induction and Multiplication Via Micropropagationcontrasting

confidence: 99%

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (Carthamus tinctorius L.) cv. NARI-H-15

Vijayakumar¹,

Ponmanickam²,

Samuel³

et al. 2017

AJPS

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“…Without a noteworthy negative side effect, BAP exerted its activity especially at higher concentrations (50-100 lM) while TDZ was already active at its lowest concentration (1 lM) but detrimental at concentrations higher than 10 lM. The higher activity towards shoot production of the ureatype cytokinin TDZ compared to adenine-type (BAP and kine) and isoprenoid-type (zea and 2iP) cytokinins, especially at lower concentrations has been reported before (Mok et al 1982;Huetteman and Preece 1993;Sunagawa et al 2007). Since we were not able to obtain somatic embryos on any of the banana shoot tips grown on different TDZ containing media, we can not confirm the property of TDZ to substitute both for auxins and cytokinins, as reported for many dicot species (Visser et al 1992;Murthy et al 1998).…”

Section: Discussionmentioning

confidence: 50%

Adventitious shoot formation is not inherent to micropropagation of banana as it is in maize

Strosse

Andre

Sági

et al. 2008

Plant Cell Tiss Organ Cult

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“…The PSK has been shown to be effective in the differentiation and cell proliferation of various plant species (Igasaki et al, 2003) and to increase the transformation efficiency of carrot hypocotyl explants (Matsubayashi et al, 2004). The transformation efficiency obtained in the present study was higher than that of reports published previously on the production of transgenic ice plant (whole plant) (Sunagawa et al, 2007). It has been achieved by infection with Agrobacterium strain EHA105 harboring a binary vector pCAMBIA.…”

Section: Introductioncontrasting

confidence: 59%

“…Direct organogenesis, which may possess less probability of somaclonal variation, may be more appropriate as a regeneration strategy for Agrobacterium-mediated ice plant transformation. We have developed a transformation procedure using Agrobacterium-mediated transformation of cotyledonary node explants coupled with the regeneration of transformed plants via direct organogenesis from cotyledonary node (Sunagawa, Agarie, Umemoto, Makishi & Nose, 2007). In the present study, we have examined the effects of different Agrobacterium strains and binary vectors on the efficiency of gene transfer and the effects of cytokinin, phytosulfokine (PSK) (Matsubayashi, Goto & Sakagami, 2004), NaCl and antibiotics on the efficiency of regeneration of transgenic ice plants.…”

Section: Introductionmentioning

confidence: 99%

AnAgrobacterium-mediated transformation via organogenesis regeneration of a facultative CAM plant, the common ice plantMesembryanthemum crystallinumL

Agarie

Umemoto

Sunagawa

et al. 2020

Plant Production Science

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The common ice plant, Mesembryanthemum crystallinum L. provides a useful model for the study of environmentally induced photosynthetic conversion and abiotic stresses tolerance. However, a procedure for the production of transgenic ice plant, which is essential for functional genomics, has not been fully established. Here we tested the factors on the transformation of cotyledonary nodes excised from the ice plant seedlings such as thidiazuron (TDZ), NaCl and phytosulfokine (PSK), a peptidyl plant growth factor using Agrobacterium tumefaciens strains EHA101 and EHA105 harboring binary vector plasmids pBI7EGFP and pCAMBIA1302, respectively. The established procedure is as follows: the explants (cotyledonary nodes) were co-cultivated with Agrobacterium for 3 days, and the explants were cultured in the medium with 0.5 mg l −1 kinetin and 100 mg l −1 carbenicillin for 72 h, and they were cultured in the medium with 0.5 mg l −1 kinetin and 100 nM PSKfor 4 weeks. Thidiazuron and NaCl enhanced the production of multiple adventitious shoot formation during regeneration but reduced the transformation efficiency due to the vitrification of adventitious shoots. PSK was effective in the production of healthy adventitious shoots. The transformation frequency at the stage of whole plants was 0.6% and 4.6% per inoculated cotyledonary nodes using the Agrobacterium strain EHA101 (pBI7EGFP) and EHA105 (pCAMBIA1302), respectively. ARTICLE HISTORY

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Effect of Urea-Type Cytokinins On The Adventitious Shoots Regeneration From Cotyledonary Node Explant in Thecommon Ice Plant,Mesembryanthemum Crystallinum

Cited by 18 publications

References 35 publications

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (<i>Carthamus tinctorius</i> L.) cv. NARI-H-15

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (<i>Carthamus tinctorius</i> L.) cv. NARI-H-15

Adventitious shoot formation is not inherent to micropropagation of banana as it is in maize

AnAgrobacterium-mediated transformation via organogenesis regeneration of a facultative CAM plant, the common ice plantMesembryanthemum crystallinumL

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Effect of Urea-Type Cytokinins On The Adventitious Shoots Regeneration From Cotyledonary Node Explant in Thecommon Ice Plant,Mesembryanthemum Crystallinum

Cited by 18 publications

References 35 publications

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (&lt;i&gt;Carthamus tinctorius&lt;/i&gt; L.) cv. NARI-H-15

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (&lt;i&gt;Carthamus tinctorius&lt;/i&gt; L.) cv. NARI-H-15

Adventitious shoot formation is not inherent to micropropagation of banana as it is in maize

AnAgrobacterium-mediated transformation via organogenesis regeneration of a facultative CAM plant, the common ice plantMesembryanthemum crystallinumL

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Product

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Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (<i>Carthamus tinctorius</i> L.) cv. NARI-H-15

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (<i>Carthamus tinctorius</i> L.) cv. NARI-H-15