Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.)

Abstract

Using cotyledon explants excised from seedlings germinated in vitro, an efficient plant regeneration system via organogenesis was established for bottle gourd (Lagenaria siceraria Standl.). Maximum shoot regeneration was obtained when the proximal parts of cotyledons from 4-day-old seedlings were cultured on MS medium with 3 mg/l BA and 0.5 mg/l AgNO(3) under a 16-h photoperiod. After 3-4 weeks of culture, 21.9-80.7% of explants from the five cultivars regenerated shoots. Adventitious shoots were successfully rooted on a half-strength MS medium with 0.1 mg/l IAA for 2-3 weeks. Flow cytometric analysis revealed that most of the regenerated plants derived from culture on medium with AgNO(3) were diploid.

Synergistic Effect of Kinetin and Benzyl Adenine Plays a Vital Role in High Frequency Regeneration from Cotyledon Explants of Bottle Gourd (Lagenaria siceraria) in Relation to Ethylene Production

“…Although plant growth regulator modifications during the culture period are important for successful regeneration, the major determining factor for regeneration competence was associated with optimal explant age. These results were in good agreement with previous reports on bottle gourd (Han et al 2004), cucumber (Mohiuddin et al 1997) and squash . A possible explanation is that the proximal part of young cotyledons of 4-day-old seedlings was very active physiologically and was easily affected by environmental factors, such as the presence of exogenous hormones.…”

“…Our results are consistent with this observation, since shoot length significantly increased on media containing kinetin. According to the previous study of Han et al (2004), only BA was considered to be an essential factor for adventitious shoot regeneration of bottle gourd and the use of AgNO 3 , a potent ethylene action inhibitor, increased the regeneration percentage from 37.5% to 52.4%. The results obtained in our study are compatible with this finding, and also clearly indicate that the use of AgNO 3 is more effective on BA.…”

Germinação e propagação in vitro de porongo

“…Em média, 73% dos explantes cotiledonares produziram brotações adventícias, com uma média de 1,1 brotação por explante. Esses resultados discordam, em parte, dos resultados obtidos por HAN et al (2004) com a cultivar de porongo Partner. A regeneração de explantes cotiledonares foi de 52,4% com uma média de 3,5 brotações por explante, obtidas somente com a adição de 3mg L -1 de BAP.…”

“…Brotações adventícias também foram induzidas sem a adição de reguladores de crescimento nas cultivares "Inodorus" (STIPP et al, 2001) e "Galia" de meloeiro (SPETSIDIS et al, 1996) e "Sürme" de melancia (PIRINÇ et al, 2003). Além das diferenças genéticas entre espécies de cucurbitáceas, variações entre 21,9 a 80,7% de explantes cotiledonares com 1,6 a 3,5 brotações adventícias por explante foram observadas em cinco cultivares de porongo (HAN et al, 2004 (SILVA et al, 2006). O maior crescimento observado nos ápices caulinares pode estar associado ao nível endógeno de auxinas, responsáveis pelo alongamento e crescimento de caules (TAIZ & ZEIGER, 2004).…”

“…A cuia é o recipiente utilizado para o preparo do chimarrão, bebida originária dos nativos do sul da América Latina e ainda hoje muito apreciada (BISOGNIN & MARCHEZAN, 1988). A excelente tolerância à baixa temperatura e a alta resistência a patógenos de solo possibilita que o porongo seja utilizado como porta-enxerto de melancia (Citrullus lanatus) e abóbora (Cucurbita pepo) em ambientes protegidos (HAN et al, 2004 (WANG et al, 2001). A cultura de anteras permite a obtenção de plantas haplóides androgenéticas, o que permite a obtenção de linhagens homozigóticas em uma única etapa (ANDRADE, 1998).…”