Synergistic Effect of Kinetin and Benzyl Adenine Plays a Vital Role in High Frequency Regeneration from Cotyledon Explants of Bottle Gourd (Lagenaria siceraria) in Relation to Ethylene Production

Saha, Shyamali; Mori, Hitoshi; Hattori, Katsumi

doi:10.1270/jsbbs.57.197

Cited by 25 publications

(19 citation statements)

References 24 publications

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“…Less fold difference is obtained on average shoot length of using TDZ instead of Kn, but with two fold higher concentration of TDZ than Kn. In contrast to our results, BAP was reported in literature as the most effective cytokinin for shoot induction and multiplication in cucurbits (Ananthakrishnan et al, 2003;Saha et al, 2007;Ganasan & Huyop, 2010) The percentage of regeneration using Kn is a at least a fold and half more than the percentage of regeneration using any of the other three types of cytokinin. After obtaining the highest physiological response, the percentage of regeneration is not inhibited much by increasing the concentration of cytokinin regardless of its type.…”

Section: Resultscontrasting

confidence: 99%

Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber

Abu-Romman¹,

Al-Hadid²,

Arabiyyat³

2015

JAS

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In vitro shoot multiplication of cucumber (Cucumis sativus) was examined from the nodal explants of 10-day-old aseptic plantlets using Murashige and Skoog (MS) media supplemented with different concentration (0, 0.5, 1, 2, 3 mg L -1 ) of cytokinins (6-Benzylaminopurine-BAP, Kinetin-Kn, Thidiazuron-TDZ, and Zeatin). Nodal explants of cucumber showed shoot induction and multiplication in response to all cytokinins tried. MS medium containing Kn was the most effective for inducing shoots from nodal explants of cucumber. The maximum rate of regeneration (83%), the highest number of obtained shoots (7.93 shoots/explant) and the longest shoots (3.61 cm) were obtained on MS medium fortified with 1 mg L -1 Kn. The lowest culture responses were recorded for media supplemented with either BAP or Zeatin. In conclusion, using Kn is strongly recommended than using Zeatin, TDZ and BAP to obtain the highest percentage of regeneration, the highest number of shoot/explant, and the highest shoot length for nodal explants of cucumber.

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Section: Resultscontrasting

confidence: 99%

Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber

Abu-Romman¹,

Al-Hadid²,

Arabiyyat³

2015

JAS

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“…Our study revealed that the effect of BAP and Kn combination on multiple shoot induction is more compared to BAP or Kn when used separately. This is in confirmation with the earlier studies in Bambusa glaucescens (Shirin and Rana, 2007) and Lagenaria siceraria (Saha et al, 2007). Srivastava and Joshi (2009) reported that BAP tested individually was more effective than Kn alone on shoot multiplication of Portulaca grandiflora but their combination was most advisable for shoot multiplication.…”

Section: Resultssupporting

confidence: 89%

In vitro micropropagation of Orthosiphon aristatus (Blume) Miq.

Reshi¹,

Sudarshana²

2015

J. Med. Plants Res.

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The present study deals with the mass propagation of Orthosiphon aristatus -a multipurpose highly valuable ethnomedicinal shrub. Stem and inflorescence explants were inoculated into the Murashige and Skoog medium (MS medium) fortified with different concentrations and combinations of auxins and cytokinins. The maximum number of shoots achieved was 25 ± 0.78 and 17 ± 0.67 at 5 mg/l benzylaminopurine (BAP) + 2.5 mg/l kinetin (kn) and 3mg/l BAP + 1.5mg/l Kn from nodal and inflorescence explants, respectively. For in vitro rhizogenesis, the elongated micro shoots were aseptically excised and were transferred to the half strength MS liquid medium supplemented with different concentrations of Indole-3 acetic acid and Indole butyric acid (IAA and IBA). The best rooting was achieved at 2 mg/l IBA giving maximum of 12 ± 0.67 roots per shoot. The well established plantlets were hardened by transferring them to the poly cups containing sterile soil and vermicompost in the ratio of 1:1. After 3 weeks of hardening, established plantlets were transferred to the field with 95% survivability.

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“…In the present study our results revealed that the effect of BAP and auxin combination on multiple shoot induction is more compared to BAP or auxins when used separately. This is in confirmation with the earlier studies in Bambusa glaucescens (Shirin and Rana, 2007) and Lagenaria siceraria (Saha et al, 2007). For in vitro rhizogenesis, IBA at 2 mg/l concentration induced maximum number of roots while simultaneously improving the growth of micro shoots.…”

Section: Discussionsupporting

confidence: 92%

In Vitro Micropropagation of Anisochilus carnosus (L) Wall

2017

J App Pharm Sci

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The present study describes a protocol for the in vitro mass propagation of Anisochilus carnosus (L) Wall -an anticancer ethnomedicinal herb. Young and healthy leaf segments were inoculated on to the solid Murashige and Skoog (MS) medium supplemented with different auxins and cytokinins individually and in combination. Maximum callusing percentage (97%) was achieved in MS medium fortified with 2,4-Dichlorophenoxyacetic acid (2,4-D) at 5 mg/l. Multiple shoots were achieved in benzylaminopurine (BAP) and Indole-3 acetic acid (IAA) combination with maximum of 14±0.23 shoots per culture at 7 mg/l BAP + 3.5 mg/l IAA concentration. For in vitro rhizogenesis, elongated micro shoots were aseptically excised and transferred to the half strength MS liquid medium enriched with different auxins. Out of the different auxins tested, indole butyric acid (IBA) was found to be the best for rhizogenesis at 2 mg/l with maximum of 11±0.45 roots per shoot. The well established rooted plantlets were hardened under laboratory conditions for two weeks by transferring to the poly cups containing vermicompost and sterile soil in the ratio of 1:1. The hardened plantlets were transferred to the green house for two weeks and finally to the filed with 90 % survivability.

show abstract

Synergistic Effect of Kinetin and Benzyl Adenine Plays a Vital Role in High Frequency Regeneration from Cotyledon Explants of Bottle Gourd (Lagenaria siceraria) in Relation to Ethylene Production

Cited by 25 publications

References 24 publications

Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber

Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber

In vitro micropropagation of Orthosiphon aristatus (Blume) Miq.

In Vitro Micropropagation of Anisochilus carnosus (L) Wall

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