Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench

Oldach, Klaus; Morgenstern, Anika; Rother, S.; Girgi, Maram; O’Kennedy, Martha M.; Lörz, Horst

doi:10.1007/s002990100335

Cited by 41 publications

(16 citation statements)

References 21 publications

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“…(Oldach et al, 2001 ;Sharma et al, 2005). Au-delà de deux mois, toutes les plantes régénérées sont albinos (Gamborg et al, 1970;Lu et al, 1981).…”

Section: Discussionunclassified

“…En ce qui concerne le mil, Pennisetum glaucum ou Pennisetum americanum, plusieurs travaux ont permis la régénération de plantes à partir de suspensions cellulaires obtenues avec différents explants , 1982Vasil, 1994;Devi et al, 2000;Oldach et al, 2001;Arockiasamy et al, 2006;Jha et al, 2009). Dans toutes ces études, l'apex caulinaire n'est généralement pas l'explant utilisé et, lorsque c'est le cas (Lambé et al, 1995;, la régénération de plantes à partir des suspensions cellulaires n'a pas fait l'objet d'étude approfondie.…”

Section: Introductionunclassified

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Optimisation des conditions de régénération de plantes fertiles à partir de suspensions cellulaires issues de cals embryogenèse d’apex caulinaire de mil (<i>Pennisetum glaucum</i> (L.) R.)

Tiecoura¹,

Alla-N'Nan²,

Kouassi³

et al. 2015

Int. J. Bio. Chem. Sci

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RÉSUMÉCe travail a pour objectif d'établir les conditions optimales de régénération de plantes fertiles à partir de suspensions cellulaires embryogènes en vue de leur utilisation pour une transformation génétique par bombardements de protoplastes. Ce travail réalisé sur des variétés de Pennisetum glaucum de Côte d'Ivoire a permis de mettre en évidence les facteurs qui influent (taille et âge des cals, milieu de conditonnement, et âge de la suspension cellulaire) sur la régénération de plantes fertiles. Il ressort de cette étude que la taille des cals et les milieux de conditionnement utilisés n'ont aucun effet sur le taux de régénération de plantes fertiles. Cependant, l'âge de la suspension cellulaire obtenue à partir de cals embryogènes et l'âge du cal initiateur de la suspension cellulaire influent sur le taux de régénération. Le taux de plantes normales régénérées est élevé durant les deux premiers mois de culture de la suspension cellulaire initiée à partir des cals de 2-3 mois (60 à 70%). Les plantes régénérées présentent les mêmes caractéristiques de croissance végétative (taille, longueur de la chandelle, nombre de barres, longueur de l'épi) que les plantes témoins. Pour le rendement, les plantes régénérées portent moins de grains (412) que les plantes témoins (715).

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“…(Oldach et al, 2001 ;Sharma et al, 2005). Au-delà de deux mois, toutes les plantes régénérées sont albinos (Gamborg et al, 1970;Lu et al, 1981).…”

Section: Discussionunclassified

Section: Introductionunclassified

Optimisation des conditions de régénération de plantes fertiles à partir de suspensions cellulaires issues de cals embryogenèse d’apex caulinaire de mil (<i>Pennisetum glaucum</i> (L.) R.)

Tiecoura¹,

Alla-N'Nan²,

Kouassi³

et al. 2015

Int. J. Bio. Chem. Sci

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“…The phenomenon of multiple copy integration is commonly encountered in particle-bombardment-mediated genetic transformation of plants [15][16][17][18]. In earlier studies, a variable number of incorporated transgene copies ranging between 1 and 20 were reported in diverse crop plants [19][20][21][22][23][24][25][26][27][28][29][30].…”

Section: Figure: 7 Extraction Of Total Rna and Rt-pcr Analysismentioning

confidence: 99%

In vitro Regeneration and Optimization of Conditions for transformation methods in Pearl millet, Pennisetum glaucum (L.)

Naravula¹,

Maheshwari²,

Naidu³

et al. 2016

IJCBS

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Pearl millet is a dual-purpose crop used for grain and fodder and is grown primarily in Asia and Africa, where it occupies some 27 million ha. It is capable of growing on some of the poorest soils in dry, hot regions of Africa and Asia, where, as a poor man's source of dietary energy, it sustains a large proportion of the populace. It is also grown in other countries where, under relatively more favorable conditions, it provides grain for bullocks, dairy cows, and poultry. Downy mildew caused by Sclerospora graminicola (Sacc.) J. Schroet. is the most widespread and destructive disease of pearl millet causing severe economic losses. New genes can be introduced into this plant through Agrobacterium mediated and bombardment genetic transformation for its genetic improvement, which is dependent on the availability of suitable in vitro techniques. An efficient regeneration system has been developed for in vitro culture of pearl millet (Pennisetum glaucum L.) from the immature inflorescence. High frequency callus and shoot regeneration was obtained on Murashige and Skoog nutrient agar medium supplemented with 2mg/l 2,4-D and 0.2 mg/l NAA, 2 mg/l Kinetin and 30 g/l sucrose. On transfer to soil, the regenerated plantlets survived and appeared to be morphologically similar to the normal seed-grown plants. Histological analysis revealed the de novo origin of shoots from embryogenic callus in in vitro cultured pearl millet. Parameters affecting transformation were optimized by assaying phosphinothricin resistance to transformed calli and basta test for these leaves of plantlets after transferring to pots. These tissues appear to be susceptible to Agrobacterium infection and Particle gun flow mediated transformation carrying pCAMBIA2300 with osmotin and chitinase double construct and pPUR with bar genes, as well as shoot multiplication. The embryogenic callus was found competent to take up the DNA, which was monitored by transient bar gene with GV2600 at 0.6 O.D for Agrobacterium infection and 1µg/µl plasmid DNA from E.coli for cobombardment was found to be compatible in giving transgenics.

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“…Many reports have shown the positive effect of AgNO 3 on plant tissue culture viz. cucumber (Mohiuddin et al, 1997); apple (Ma et al, 1998); Chinese cabbage (Zhang et al, 1998); cassava (Zang et al, 2001); pearl millet and sorghum (Oldach et al, 2001); date palm (Al- Khayri and Al-Bahrany, 2004) and rapeseed (Akasaka-Kennedy, 2005). AgNO 3 at the concentration of 11 µM was very beneficial to shoot regeneration along with the number of microrhizome production in C. longa var.…”

Section: Effect Of Silver Nitrate On In Vitro Microrhizome Productionmentioning

confidence: 99%

Silver Nitrate and Different Culture Vessels Influence High Frequency Microrhizome Induction In Vitro and Enhancement Growth of Turmeric Plantlet During Ex Vitro Acclimatization

et al. 2012

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Eleven cultivars of C. longa var. Lakadong were collected from Manipur having different topography. Curcumin content in different cultivars has been analyzed by using UV-Visible Spectrophotometer (100 Bio-Carry Spectrophotometer). The curcuminoids content were analyzed and quantified for identification of best quality cultivar. Thoubal Cultivar with highest curcumin content (9.44%) was subjected for tissue culture technique using different culture vessels and silver nitrate for rapid multiplication and scaling up of microrhizome production. High multiplication rate of 27.40±0.47 were obtained in Murashige and Skoog's medium supplemented with 3% sucrose + 1 mg L -1 α-napthalene acetic acid, 4 mg L -1 6-benzyl-amino-purine and 11 µM silver nitrate. Effect of different culture vessels and silver nitrate were studied for microrhizome and multiple shoots formation. Relatively higher rate of shoots along with microrhizome (17.5±0.32) can be seen in Growtek which was grown without any plant growth regulator. Growtek was used for scaling up of microrhizome production in vitro and utmost microrhizome was produced in liquid Murashige and Skoog's medium supplemented with 8% sucrose, 1 mg L -1 α-napthalene acetic acid, 4 mg L -1 6-benzyl-amino-purine and 11 µM silver nitrate (36.25±0.27). Addition of silver nitrate in the medium resulted in improvement of microrhizome induction in vitro. Higher concentration of silver nitrate (33, 44, 66, 88 µM) negatively affected the microrhizome and shoot multiplication and shows inhibition of tissue response completely. Analysis of in vitro derived plantlets during acclimatization shows that the exogenous applied of silver nitrate shows superior growth as compared to control. 90-95% of plantlets with and 75-80% plantlets without silver nitrate treatment were successfully established under ex vitro acclimatization. The protocol could be utilized for large scale production of true-to-type plantlets and as alternative method to step forward towards an improved commercial propagation system for more efficient and productivity.

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Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench

Cited by 41 publications

References 21 publications

Optimisation des conditions de régénération de plantes fertiles à partir de suspensions cellulaires issues de cals embryogenèse d’apex caulinaire de mil (<i>Pennisetum glaucum</i> (L.) R.)

Optimisation des conditions de régénération de plantes fertiles à partir de suspensions cellulaires issues de cals embryogenèse d’apex caulinaire de mil (<i>Pennisetum glaucum</i> (L.) R.)

In vitro Regeneration and Optimization of Conditions for transformation methods in Pearl millet, Pennisetum glaucum (L.)

Silver Nitrate and Different Culture Vessels Influence High Frequency Microrhizome Induction In Vitro and Enhancement Growth of Turmeric Plantlet During Ex Vitro Acclimatization

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